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Inactivation of NADP(+)-dependent isocitrate dehydrogenase by reactive oxygen species.
Biochimie. 2001 Nov-Dec; 83(11-12):1057-65.B

Abstract

Recently, we demonstrated that the control of cytosolic and mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of NADP(+)-dependent isocitrate dehydrogenase (ICDH) through supply of NADPH for antioxidant systems. When exposed to various reactive oxygen species such as hydrogen peroxide, singlet oxygen generated by photoactivated dye, superoxide anion, and hydroxyl radical produced by metal-catalyzed Fenton reactions, ICDH was susceptible to oxidative modification and damage, which was indicated by the loss of activity, fragmentation of the peptide as well as by the formation of carbonyl groups. Oxidative damage to ICDH was inhibited by antioxidant enzymes, free radical scavengers, and spin-trapping agents. The structural alterations of modified enzymes were indicated by the increase in thermal instability and binding of the hydrophobic probe 8-anilino-1-naphthalene sulfonic acid (ANSA). The reactive oxygen species-mediated damage to ICDH may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, South Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11879734

Citation

Lee, S M., et al. "Inactivation of NADP(+)-dependent Isocitrate Dehydrogenase By Reactive Oxygen Species." Biochimie, vol. 83, no. 11-12, 2001, pp. 1057-65.
Lee SM, Huh TL, Park JW. Inactivation of NADP(+)-dependent isocitrate dehydrogenase by reactive oxygen species. Biochimie. 2001;83(11-12):1057-65.
Lee, S. M., Huh, T. L., & Park, J. W. (2001). Inactivation of NADP(+)-dependent isocitrate dehydrogenase by reactive oxygen species. Biochimie, 83(11-12), 1057-65.
Lee SM, Huh TL, Park JW. Inactivation of NADP(+)-dependent Isocitrate Dehydrogenase By Reactive Oxygen Species. Biochimie. 2001 Nov-Dec;83(11-12):1057-65. PubMed PMID: 11879734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation of NADP(+)-dependent isocitrate dehydrogenase by reactive oxygen species. AU - Lee,S M, AU - Huh,T L, AU - Park,J W, PY - 2002/3/7/pubmed PY - 2002/5/22/medline PY - 2002/3/7/entrez SP - 1057 EP - 65 JF - Biochimie JO - Biochimie VL - 83 IS - 11-12 N2 - Recently, we demonstrated that the control of cytosolic and mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of NADP(+)-dependent isocitrate dehydrogenase (ICDH) through supply of NADPH for antioxidant systems. When exposed to various reactive oxygen species such as hydrogen peroxide, singlet oxygen generated by photoactivated dye, superoxide anion, and hydroxyl radical produced by metal-catalyzed Fenton reactions, ICDH was susceptible to oxidative modification and damage, which was indicated by the loss of activity, fragmentation of the peptide as well as by the formation of carbonyl groups. Oxidative damage to ICDH was inhibited by antioxidant enzymes, free radical scavengers, and spin-trapping agents. The structural alterations of modified enzymes were indicated by the increase in thermal instability and binding of the hydrophobic probe 8-anilino-1-naphthalene sulfonic acid (ANSA). The reactive oxygen species-mediated damage to ICDH may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition. SN - 0300-9084 UR - https://www.unboundmedicine.com/medline/citation/11879734/Inactivation_of_NADP_+__dependent_isocitrate_dehydrogenase_by_reactive_oxygen_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300908401013517 DB - PRIME DP - Unbound Medicine ER -