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Molecular cloning and expression of thyroid hormone receptor alpha during salmonid development.
Gen Comp Endocrinol 2002; 125(2):226-35GC

Abstract

Thyroid hormones have been implicated as important regulators of teleost development. To gain a better understanding of the potential roles of the thyroid system in salmonids a genomic clone which encoded rainbow trout TR-alpha was isolated. This clone exhibited highest amino acid identity to Japanese flounder TR-alphaB (94%) and zebrafish TR-alpha1 (94%). Oligonucleotides were designed against the rainbow trout sequence and the complete coding region of Atlantic salmon TR-alpha was isolated by RACE-PCR. The Atlantic salmon sequence exhibited highest amino acid identity to rainbow trout TR-alpha (98%), Japanese flounder TR-alphaB (93%), and zebrafish TR-alpha1 (90%). Atlantic salmon TR-alpha exhibited the classic modular structure associated with members of the nuclear receptor superfamily and consisted of a divergent A/B domain while the DNA and ligand-binding domains were highly conserved to other teleost TR proteins. Temporal expression from the rainbow trout TR-alpha gene was monitored by semiquantitative RT-PCR at selected stages during rainbow trout embryonic and larval development. High levels of maternal transcripts were present at cleavage (Stage 6) which were rapidly degraded by gastrulation (Stage 13). Low levels of TR-alpha expression were then detected during organogenesis (Stages 20, 24, 26, 29, and 31). A peak in mRNA levels was observed at hatch (Stage 32) after which levels rose in a gradual manner during larval development (Stages 33, 34, 35, and 36) to reach maximal values at first feeding (Stage 37). These results suggest that the thyroid axis is functional and that embryonic and larval rainbow trout are at least capable of responding to thyroid hormones. These observations implicate the thyroid system as being an important regulator of salmonid development.

Authors+Show Affiliations

Cardiff School of Biosciences, Cardiff University, Biomedical Sciences Building, Cardiff, CF10 3US, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11884068

Citation

Jones, Iwan, et al. "Molecular Cloning and Expression of Thyroid Hormone Receptor Alpha During Salmonid Development." General and Comparative Endocrinology, vol. 125, no. 2, 2002, pp. 226-35.
Jones I, Rogers SA, Kille P, et al. Molecular cloning and expression of thyroid hormone receptor alpha during salmonid development. Gen Comp Endocrinol. 2002;125(2):226-35.
Jones, I., Rogers, S. A., Kille, P., & Sweeney, G. E. (2002). Molecular cloning and expression of thyroid hormone receptor alpha during salmonid development. General and Comparative Endocrinology, 125(2), pp. 226-35.
Jones I, et al. Molecular Cloning and Expression of Thyroid Hormone Receptor Alpha During Salmonid Development. Gen Comp Endocrinol. 2002 Feb 1;125(2):226-35. PubMed PMID: 11884068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and expression of thyroid hormone receptor alpha during salmonid development. AU - Jones,Iwan, AU - Rogers,Sarah A, AU - Kille,Peter, AU - Sweeney,Glen E, PY - 2002/3/9/pubmed PY - 2002/6/25/medline PY - 2002/3/9/entrez SP - 226 EP - 35 JF - General and comparative endocrinology JO - Gen. Comp. Endocrinol. VL - 125 IS - 2 N2 - Thyroid hormones have been implicated as important regulators of teleost development. To gain a better understanding of the potential roles of the thyroid system in salmonids a genomic clone which encoded rainbow trout TR-alpha was isolated. This clone exhibited highest amino acid identity to Japanese flounder TR-alphaB (94%) and zebrafish TR-alpha1 (94%). Oligonucleotides were designed against the rainbow trout sequence and the complete coding region of Atlantic salmon TR-alpha was isolated by RACE-PCR. The Atlantic salmon sequence exhibited highest amino acid identity to rainbow trout TR-alpha (98%), Japanese flounder TR-alphaB (93%), and zebrafish TR-alpha1 (90%). Atlantic salmon TR-alpha exhibited the classic modular structure associated with members of the nuclear receptor superfamily and consisted of a divergent A/B domain while the DNA and ligand-binding domains were highly conserved to other teleost TR proteins. Temporal expression from the rainbow trout TR-alpha gene was monitored by semiquantitative RT-PCR at selected stages during rainbow trout embryonic and larval development. High levels of maternal transcripts were present at cleavage (Stage 6) which were rapidly degraded by gastrulation (Stage 13). Low levels of TR-alpha expression were then detected during organogenesis (Stages 20, 24, 26, 29, and 31). A peak in mRNA levels was observed at hatch (Stage 32) after which levels rose in a gradual manner during larval development (Stages 33, 34, 35, and 36) to reach maximal values at first feeding (Stage 37). These results suggest that the thyroid axis is functional and that embryonic and larval rainbow trout are at least capable of responding to thyroid hormones. These observations implicate the thyroid system as being an important regulator of salmonid development. SN - 0016-6480 UR - https://www.unboundmedicine.com/medline/citation/11884068/Molecular_cloning_and_expression_of_thyroid_hormone_receptor_alpha_during_salmonid_development_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016648001977457 DB - PRIME DP - Unbound Medicine ER -