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Melanocyte-associated T cell epitopes can function as autoantigens for transfer of alopecia areata to human scalp explants on Prkdc(scid) mice.
J Invest Dermatol. 2001 Dec; 117(6):1357-62.JI

Abstract

Alopecia areata is a tissue restricted autoimmune condition affecting the hair follicle, resulting in hair loss. The goal of this study was to test the hypothesis that the autoantigen of alopecia areata is melanocyte associated. Potential autoantigens were tested in the human scalp explant/Prkd(scid) CB-17 mouse transfer system. Scalp T cells from lesional (bald) alopecia areata scalp were cultured with antigen-presenting cells, and antigen, along with interleukin-2. The T cells were then injected into autologous lesional scalp grafts on SCID mice, and hair regrowth was measured. Hair follicle homogenate was used as an autoantigen control. T cells cultured with melanoma homogenate induced statistically significant reduction in hair growth (p <0.01 by ANOVA). HLA-A2-restricted melanocyte peptide epitopes were then tested with lesional scalp T cells from HLA-A2-positive alopecia areata patients. Melanocyte-peptide-activated T cells significantly reduced the number of hairs regrowing in two experiments with six patients (p <0.001 by ANOVA). Injected scalp grafts showed histologic and immunochemical changes of alopecia areata. The most consistent peptide autoantigens were the Gp100-derived G9-209 and G9-280 peptides, as well as MART-1 (27-35). Melanocyte peptide epitopes can function as autoantigens for alopecia areata. Multiple peptides were recognized, suggesting epitope spreading.

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Authors+Show Affiliations

Gilhar A
Skin Research Laboratories, Flieman Medical Center and Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa, Israel.
Landau M
No affiliation info available
Assy B
No affiliation info available
Shalaginov R
No affiliation info available
Serafimovich S
No affiliation info available
Kalish RS
No affiliation info available

MeSH

AdultAlopecia AreataAnimalsAutoantigensCell ExtractsEpitopes, T-LymphocyteFemaleHLA-A2 AntigenHair FollicleHumansMaleMelanocytesMelanomaMiceMice, SCIDScalpT-LymphocytesTumor Cells, Cultured

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11886495

Citation

Gilhar, A, et al. "Melanocyte-associated T Cell Epitopes Can Function as Autoantigens for Transfer of Alopecia Areata to Human Scalp Explants On Prkdc(scid) Mice." The Journal of Investigative Dermatology, vol. 117, no. 6, 2001, pp. 1357-62.
Gilhar A, Landau M, Assy B, et al. Melanocyte-associated T cell epitopes can function as autoantigens for transfer of alopecia areata to human scalp explants on Prkdc(scid) mice. J Invest Dermatol. 2001;117(6):1357-62.
Gilhar, A., Landau, M., Assy, B., Shalaginov, R., Serafimovich, S., & Kalish, R. S. (2001). Melanocyte-associated T cell epitopes can function as autoantigens for transfer of alopecia areata to human scalp explants on Prkdc(scid) mice. The Journal of Investigative Dermatology, 117(6), 1357-62.
Gilhar A, et al. Melanocyte-associated T Cell Epitopes Can Function as Autoantigens for Transfer of Alopecia Areata to Human Scalp Explants On Prkdc(scid) Mice. J Invest Dermatol. 2001;117(6):1357-62. PubMed PMID: 11886495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melanocyte-associated T cell epitopes can function as autoantigens for transfer of alopecia areata to human scalp explants on Prkdc(scid) mice. AU - Gilhar,A, AU - Landau,M, AU - Assy,B, AU - Shalaginov,R, AU - Serafimovich,S, AU - Kalish,R S, PY - 2002/3/12/pubmed PY - 2002/4/6/medline PY - 2002/3/12/entrez SP - 1357 EP - 62 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 117 IS - 6 N2 - Alopecia areata is a tissue restricted autoimmune condition affecting the hair follicle, resulting in hair loss. The goal of this study was to test the hypothesis that the autoantigen of alopecia areata is melanocyte associated. Potential autoantigens were tested in the human scalp explant/Prkd(scid) CB-17 mouse transfer system. Scalp T cells from lesional (bald) alopecia areata scalp were cultured with antigen-presenting cells, and antigen, along with interleukin-2. The T cells were then injected into autologous lesional scalp grafts on SCID mice, and hair regrowth was measured. Hair follicle homogenate was used as an autoantigen control. T cells cultured with melanoma homogenate induced statistically significant reduction in hair growth (p <0.01 by ANOVA). HLA-A2-restricted melanocyte peptide epitopes were then tested with lesional scalp T cells from HLA-A2-positive alopecia areata patients. Melanocyte-peptide-activated T cells significantly reduced the number of hairs regrowing in two experiments with six patients (p <0.001 by ANOVA). Injected scalp grafts showed histologic and immunochemical changes of alopecia areata. The most consistent peptide autoantigens were the Gp100-derived G9-209 and G9-280 peptides, as well as MART-1 (27-35). Melanocyte peptide epitopes can function as autoantigens for alopecia areata. Multiple peptides were recognized, suggesting epitope spreading. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/11886495/Melanocyte_associated_T_cell_epitopes_can_function_as_autoantigens_for_transfer_of_alopecia_areata_to_human_scalp_explants_on_Prkdc_scid__mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)41470-8 DB - PRIME DP - Unbound Medicine ER -