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The immunophenotype of 325 adult acute leukemias: relationship to morphologic and molecular classification and proposal for a minimal screening program highly predictive for lineage discrimination.
Am J Clin Pathol. 2002 Mar; 117(3):380-9.AJ

Abstract

Bone marrow cells of 325 adults with acute leukemia were immunophenotyped using a panel of monoclonal antibodies proposed by the European Group for the Immunological Characterization of Leukemias (EGIL). Of these, 97.2% could be assigned clearly to myeloid or lymphoid lineage (254 acute myeloid leukemias [AMLs], 48 B-cell lineage acute lymphoblastic leukemias [ALLs], 14 T-cell lineage ALLs), 1.8% as biphenotypic, and less than 1% as undifferentiated. Immunologic subtyping of ALLs revealed an association between early precursor phenotypes and coexpression of myeloid antigens, particularly CD15/CD65s coexpression and pre-pre-B cell-specific phenotypes and genotypes. The common ALL phenotype was associated with BCR-ABL translocation. Among AMLs, CD2 coexpression was almost exclusively restricted to French-American-British subtypes M3 variant and M4Eo and related molecular aberrations. The most valuable markers to differentiate between myeloperoxidase-negative AML subtypes M0 and ALLs were CD13, CD33, and CD117, typical of M0, and intracytoplasmic CD79a, intracytoplasmic CD3, CD10, and CD2, typical of B cell- or T cell-lineage ALL. Our results confirm excellent practicability of the EGIL proposalfor immunologic classification of acute leukemias. For myeloperoxidase-negative AMLs, we suggest a scoring system based on markers most valuable to distinguish between AML-M0 and ALLs.

Authors+Show Affiliations

Department of Laboratory Medicine, University of Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11888077

Citation

Thalhammer-Scherrer, Renate, et al. "The Immunophenotype of 325 Adult Acute Leukemias: Relationship to Morphologic and Molecular Classification and Proposal for a Minimal Screening Program Highly Predictive for Lineage Discrimination." American Journal of Clinical Pathology, vol. 117, no. 3, 2002, pp. 380-9.
Thalhammer-Scherrer R, Mitterbauer G, Simonitsch I, et al. The immunophenotype of 325 adult acute leukemias: relationship to morphologic and molecular classification and proposal for a minimal screening program highly predictive for lineage discrimination. Am J Clin Pathol. 2002;117(3):380-9.
Thalhammer-Scherrer, R., Mitterbauer, G., Simonitsch, I., Jaeger, U., Lechner, K., Schneider, B., Fonatsch, C., & Schwarzinger, I. (2002). The immunophenotype of 325 adult acute leukemias: relationship to morphologic and molecular classification and proposal for a minimal screening program highly predictive for lineage discrimination. American Journal of Clinical Pathology, 117(3), 380-9.
Thalhammer-Scherrer R, et al. The Immunophenotype of 325 Adult Acute Leukemias: Relationship to Morphologic and Molecular Classification and Proposal for a Minimal Screening Program Highly Predictive for Lineage Discrimination. Am J Clin Pathol. 2002;117(3):380-9. PubMed PMID: 11888077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The immunophenotype of 325 adult acute leukemias: relationship to morphologic and molecular classification and proposal for a minimal screening program highly predictive for lineage discrimination. AU - Thalhammer-Scherrer,Renate, AU - Mitterbauer,Gerlinde, AU - Simonitsch,Ingrid, AU - Jaeger,Ulrich, AU - Lechner,Klaus, AU - Schneider,Barbara, AU - Fonatsch,Christa, AU - Schwarzinger,Ilse, PY - 2002/3/13/pubmed PY - 2002/3/26/medline PY - 2002/3/13/entrez SP - 380 EP - 9 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 117 IS - 3 N2 - Bone marrow cells of 325 adults with acute leukemia were immunophenotyped using a panel of monoclonal antibodies proposed by the European Group for the Immunological Characterization of Leukemias (EGIL). Of these, 97.2% could be assigned clearly to myeloid or lymphoid lineage (254 acute myeloid leukemias [AMLs], 48 B-cell lineage acute lymphoblastic leukemias [ALLs], 14 T-cell lineage ALLs), 1.8% as biphenotypic, and less than 1% as undifferentiated. Immunologic subtyping of ALLs revealed an association between early precursor phenotypes and coexpression of myeloid antigens, particularly CD15/CD65s coexpression and pre-pre-B cell-specific phenotypes and genotypes. The common ALL phenotype was associated with BCR-ABL translocation. Among AMLs, CD2 coexpression was almost exclusively restricted to French-American-British subtypes M3 variant and M4Eo and related molecular aberrations. The most valuable markers to differentiate between myeloperoxidase-negative AML subtypes M0 and ALLs were CD13, CD33, and CD117, typical of M0, and intracytoplasmic CD79a, intracytoplasmic CD3, CD10, and CD2, typical of B cell- or T cell-lineage ALL. Our results confirm excellent practicability of the EGIL proposalfor immunologic classification of acute leukemias. For myeloperoxidase-negative AMLs, we suggest a scoring system based on markers most valuable to distinguish between AML-M0 and ALLs. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/11888077/The_immunophenotype_of_325_adult_acute_leukemias:_relationship_to_morphologic_and_molecular_classification_and_proposal_for_a_minimal_screening_program_highly_predictive_for_lineage_discrimination_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/C38D-D8J3-JU3E-V6EE DB - PRIME DP - Unbound Medicine ER -