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Effects of transcutaneous short-term electrical stimulation on M. vastus lateralis characteristics of healthy young men.
Pflugers Arch. 2002 Mar; 443(5-6):866-74.PA

Abstract

Fifteen healthy, untrained male subjects (mean age +/- SD, 22 +/- 5 years) were used to examine the plasticity of myosin heavy chain phenotype, size, oxidative capacity and capillarization of skeletal muscle fibre types with short-term electrical stimulation (ES). Ten subjects were electro-stimulated on both quadriceps muscles with a frequency of 45-60 Hz, with 12 s of stimulation followed by 8 s of recovery for a total of 30 min per day, 3 days per week for 6 weeks. The remaining five subjects served as controls. Two vastus lateralis muscle biopsy samples were removed from each subject before (week 0) and after (week 6) ES training. A standardized exercise test on a cycle ergometer was performed by each subject before and after the experimental period and several indicators of whole-body aerobic capacity were estimated. The so-called electromyographic threshold was also determined during the tests. Muscle biopsy samples were analysed by electrophoresis, immunohistochemistry and quantitative histochemistry. Myosin heavy chain (MHC) composition, muscle fibre type distribution, fibre areas, oxidative capacity and capillaries of each fibre type were estimated. Muscular changes with ES revealed an increase of fibres expressing MHC-IIA, and a decrease of fibres expressing MHC-IIX and MHC-I, as well as an increase of the oxidative capacity and mean number of capillaries of fast-twitch (type II) fibres with minimal muscle fibre hypertrophy. These adaptations seem related to a bi-directional transformation from both MHC isoforms I and IIX towards the MHC-IIA isoform. The aerobic performance and electromyographic variables at the whole-body level were not altered by ES. These results indicate that the particular short-term ES training protocol tested in the present study induces significant adaptations in histochemical and metabolic machineries of human skeletal muscle. The results also offer new perspectives for realistic applications of ES in various clinical situations and sport training.

Authors+Show Affiliations

Departamento de Ciencias Morfológicas y Fisiología, Universidad Europea de Madrid, 28670 Villaviciosa de Odón, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11889587

Citation

Pérez, Margarita, et al. "Effects of Transcutaneous Short-term Electrical Stimulation On M. Vastus Lateralis Characteristics of Healthy Young Men." Pflugers Archiv : European Journal of Physiology, vol. 443, no. 5-6, 2002, pp. 866-74.
Pérez M, Lucia A, Rivero JL, et al. Effects of transcutaneous short-term electrical stimulation on M. vastus lateralis characteristics of healthy young men. Pflugers Arch. 2002;443(5-6):866-74.
Pérez, M., Lucia, A., Rivero, J. L., Serrano, A. L., Calbet, J. A., Delgado, M. A., & Chicharro, J. L. (2002). Effects of transcutaneous short-term electrical stimulation on M. vastus lateralis characteristics of healthy young men. Pflugers Archiv : European Journal of Physiology, 443(5-6), 866-74.
Pérez M, et al. Effects of Transcutaneous Short-term Electrical Stimulation On M. Vastus Lateralis Characteristics of Healthy Young Men. Pflugers Arch. 2002;443(5-6):866-74. PubMed PMID: 11889587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of transcutaneous short-term electrical stimulation on M. vastus lateralis characteristics of healthy young men. AU - Pérez,Margarita, AU - Lucia,A, AU - Rivero,J-l L, AU - Serrano,A L, AU - Calbet,J-A L, AU - Delgado,M A, AU - Chicharro,J L, Y1 - 2002/01/22/ PY - 2001/07/04/received PY - 2001/11/30/accepted PY - 2002/3/13/pubmed PY - 2002/7/26/medline PY - 2002/3/13/entrez SP - 866 EP - 74 JF - Pflugers Archiv : European journal of physiology JO - Pflugers Arch. VL - 443 IS - 5-6 N2 - Fifteen healthy, untrained male subjects (mean age +/- SD, 22 +/- 5 years) were used to examine the plasticity of myosin heavy chain phenotype, size, oxidative capacity and capillarization of skeletal muscle fibre types with short-term electrical stimulation (ES). Ten subjects were electro-stimulated on both quadriceps muscles with a frequency of 45-60 Hz, with 12 s of stimulation followed by 8 s of recovery for a total of 30 min per day, 3 days per week for 6 weeks. The remaining five subjects served as controls. Two vastus lateralis muscle biopsy samples were removed from each subject before (week 0) and after (week 6) ES training. A standardized exercise test on a cycle ergometer was performed by each subject before and after the experimental period and several indicators of whole-body aerobic capacity were estimated. The so-called electromyographic threshold was also determined during the tests. Muscle biopsy samples were analysed by electrophoresis, immunohistochemistry and quantitative histochemistry. Myosin heavy chain (MHC) composition, muscle fibre type distribution, fibre areas, oxidative capacity and capillaries of each fibre type were estimated. Muscular changes with ES revealed an increase of fibres expressing MHC-IIA, and a decrease of fibres expressing MHC-IIX and MHC-I, as well as an increase of the oxidative capacity and mean number of capillaries of fast-twitch (type II) fibres with minimal muscle fibre hypertrophy. These adaptations seem related to a bi-directional transformation from both MHC isoforms I and IIX towards the MHC-IIA isoform. The aerobic performance and electromyographic variables at the whole-body level were not altered by ES. These results indicate that the particular short-term ES training protocol tested in the present study induces significant adaptations in histochemical and metabolic machineries of human skeletal muscle. The results also offer new perspectives for realistic applications of ES in various clinical situations and sport training. SN - 0031-6768 UR - https://www.unboundmedicine.com/medline/citation/11889587/Effects_of_transcutaneous_short_term_electrical_stimulation_on_M__vastus_lateralis_characteristics_of_healthy_young_men_ L2 - https://dx.doi.org/10.1007/s00424-001-0769-6 DB - PRIME DP - Unbound Medicine ER -