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The in vitro antimutagenic activity of Triphala--an Indian herbal drug.
Food Chem Toxicol. 2002 Apr; 40(4):527-34.FC

Abstract

A study to evaluate an antimutagenic potential of water, chloroform and acetone extracts of Triphala has been made in an Ames histidine reversion assay using TA98 and TA100 tester strains of Salmonella typhimurium against the direct-acting mutagens, 4-nitro-o-phenylenediamine (NPD) and sodium azide, and the indirect-acting promutagen, 2-aminofluorene (2AF), in the presence of phenobarbitone-induced rat hepatic S9. A combination drug 'Triphala' - a composite mixture of Terminalia bellerica, T. chebula and Emblica officinalis, has been used in traditional system of medicine for the treatment of many malaises, such as heart ailments and hepatic diseases. The drug was sequentially extracted with water, acetone and chloroform at room temperature. The study revealed that water extract was ineffective in reducing the revertants induced by the mutagens. The results with chloroform and acetone extracts showed inhibition of mutagenicity induced by both direct and S9-dependent mutagens. A significant inhibition of 98.7% was observed with acetone extract against the revertants induced by S9-dependent mutagen, 2AF, in co-incubation mode of treatment. Various spectroscopic techniques, namely 1H-NMR, normal 13C-NMR, distortionless enhancement by polarization transfer (DEPT-90 and DEPT-135), UV and IR, are under way to identify the polyphenolic compounds from an acetone extract.

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Authors+Show Affiliations

Kaur S
Department of Botanical Sciences, Guru Nanak Dev University, 143 005, Amritsar, India.
Arora S
No affiliation info available
Kaur K
No affiliation info available
Kumar S
No affiliation info available

MeSH

AcetoneAnimalsDNA DamageLiverMagnetic Resonance SpectroscopyMaleMutagenicity TestsMutagensPhyllanthus emblicaPlant PreparationsRatsSalmonella typhimuriumSolventsTerminalia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11893411

Citation

Kaur, S, et al. "The in Vitro Antimutagenic Activity of Triphala--an Indian Herbal Drug." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 40, no. 4, 2002, pp. 527-34.
Kaur S, Arora S, Kaur K, et al. The in vitro antimutagenic activity of Triphala--an Indian herbal drug. Food Chem Toxicol. 2002;40(4):527-34.
Kaur, S., Arora, S., Kaur, K., & Kumar, S. (2002). The in vitro antimutagenic activity of Triphala--an Indian herbal drug. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 40(4), 527-34.
Kaur S, et al. The in Vitro Antimutagenic Activity of Triphala--an Indian Herbal Drug. Food Chem Toxicol. 2002;40(4):527-34. PubMed PMID: 11893411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The in vitro antimutagenic activity of Triphala--an Indian herbal drug. AU - Kaur,S, AU - Arora,S, AU - Kaur,K, AU - Kumar,S, PY - 2002/3/15/pubmed PY - 2002/5/15/medline PY - 2002/3/15/entrez SP - 527 EP - 34 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 40 IS - 4 N2 - A study to evaluate an antimutagenic potential of water, chloroform and acetone extracts of Triphala has been made in an Ames histidine reversion assay using TA98 and TA100 tester strains of Salmonella typhimurium against the direct-acting mutagens, 4-nitro-o-phenylenediamine (NPD) and sodium azide, and the indirect-acting promutagen, 2-aminofluorene (2AF), in the presence of phenobarbitone-induced rat hepatic S9. A combination drug 'Triphala' - a composite mixture of Terminalia bellerica, T. chebula and Emblica officinalis, has been used in traditional system of medicine for the treatment of many malaises, such as heart ailments and hepatic diseases. The drug was sequentially extracted with water, acetone and chloroform at room temperature. The study revealed that water extract was ineffective in reducing the revertants induced by the mutagens. The results with chloroform and acetone extracts showed inhibition of mutagenicity induced by both direct and S9-dependent mutagens. A significant inhibition of 98.7% was observed with acetone extract against the revertants induced by S9-dependent mutagen, 2AF, in co-incubation mode of treatment. Various spectroscopic techniques, namely 1H-NMR, normal 13C-NMR, distortionless enhancement by polarization transfer (DEPT-90 and DEPT-135), UV and IR, are under way to identify the polyphenolic compounds from an acetone extract. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/11893411/The_in_vitro_antimutagenic_activity_of_Triphala__an_Indian_herbal_drug_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278691501001016 DB - PRIME DP - Unbound Medicine ER -