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Depletion of cholinergic amacrine cells by a novel immunotoxin does not perturb the formation of segregated on and off cone bipolar cell projections.
J Neurosci 2002; 22(6):2265-73JN

Abstract

Cone bipolar cells are the first retinal neurons that respond in a differential manner to light onset and offset. In the mature retina, the terminal arbors of On and Off cone bipolar cells terminate in different sublaminas of the inner plexiform layer (IPL) where they form synapses with the dendrites of On and Off retinal ganglion cells and with the stratified processes of cholinergic amacrine cells. Here we first show that cholinergic processes within the On and Off sublaminas of the IPL are present early in development, being evident in the rat on the day of birth, approximately 10 d before the formation of segregated cone bipolar cell axons. This temporal sequence, as well as our previous finding that the segregation of On and Off cone bipolar cell inputs occurs in the absence of retinal ganglion cells, suggested that cholinergic amacrine cells could provide a scaffold for the subsequent in-growth of bipolar cell axons. To test this hypothesis directly, a new cholinergic cell immunotoxin was constructed by conjugating saporin, the ribosome-inactivating protein toxin, to an antibody against the vesicular acetylcholine transporter. A single intraocular injection of the immunotoxin caused a rapid, complete, and selective loss of cholinergic amacrine cells from the developing rat retina. On and Off cone bipolar cells were visualized using an antibody against recoverin, the calcium-binding protein that labels the soma and processes of these interneurons. After complete depletion of cholinergic amacrine cells, cone bipolar cell axon terminals still formed their two characteristic strata within the IPL. These findings demonstrate that the presence of cholinergic amacrine cells is not required for the segregation of recoverin-positive On and Off cone bipolar cell projections.

Authors+Show Affiliations

Department of Ophthalmology, School of Medicine, University of California, Davis, California 95616, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11896166

Citation

Gunhan, Emine, et al. "Depletion of Cholinergic Amacrine Cells By a Novel Immunotoxin Does Not Perturb the Formation of Segregated On and Off Cone Bipolar Cell Projections." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 22, no. 6, 2002, pp. 2265-73.
Gunhan E, Choudary PV, Landerholm TE, et al. Depletion of cholinergic amacrine cells by a novel immunotoxin does not perturb the formation of segregated on and off cone bipolar cell projections. J Neurosci. 2002;22(6):2265-73.
Gunhan, E., Choudary, P. V., Landerholm, T. E., & Chalupa, L. M. (2002). Depletion of cholinergic amacrine cells by a novel immunotoxin does not perturb the formation of segregated on and off cone bipolar cell projections. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 22(6), pp. 2265-73.
Gunhan E, et al. Depletion of Cholinergic Amacrine Cells By a Novel Immunotoxin Does Not Perturb the Formation of Segregated On and Off Cone Bipolar Cell Projections. J Neurosci. 2002 Mar 15;22(6):2265-73. PubMed PMID: 11896166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Depletion of cholinergic amacrine cells by a novel immunotoxin does not perturb the formation of segregated on and off cone bipolar cell projections. AU - Gunhan,Emine, AU - Choudary,Prabhakara V, AU - Landerholm,Thomas E, AU - Chalupa,Leo M, PY - 2002/3/16/pubmed PY - 2002/3/28/medline PY - 2002/3/16/entrez SP - 2265 EP - 73 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J. Neurosci. VL - 22 IS - 6 N2 - Cone bipolar cells are the first retinal neurons that respond in a differential manner to light onset and offset. In the mature retina, the terminal arbors of On and Off cone bipolar cells terminate in different sublaminas of the inner plexiform layer (IPL) where they form synapses with the dendrites of On and Off retinal ganglion cells and with the stratified processes of cholinergic amacrine cells. Here we first show that cholinergic processes within the On and Off sublaminas of the IPL are present early in development, being evident in the rat on the day of birth, approximately 10 d before the formation of segregated cone bipolar cell axons. This temporal sequence, as well as our previous finding that the segregation of On and Off cone bipolar cell inputs occurs in the absence of retinal ganglion cells, suggested that cholinergic amacrine cells could provide a scaffold for the subsequent in-growth of bipolar cell axons. To test this hypothesis directly, a new cholinergic cell immunotoxin was constructed by conjugating saporin, the ribosome-inactivating protein toxin, to an antibody against the vesicular acetylcholine transporter. A single intraocular injection of the immunotoxin caused a rapid, complete, and selective loss of cholinergic amacrine cells from the developing rat retina. On and Off cone bipolar cells were visualized using an antibody against recoverin, the calcium-binding protein that labels the soma and processes of these interneurons. After complete depletion of cholinergic amacrine cells, cone bipolar cell axon terminals still formed their two characteristic strata within the IPL. These findings demonstrate that the presence of cholinergic amacrine cells is not required for the segregation of recoverin-positive On and Off cone bipolar cell projections. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/11896166/Depletion_of_cholinergic_amacrine_cells_by_a_novel_immunotoxin_does_not_perturb_the_formation_of_segregated_on_and_off_cone_bipolar_cell_projections_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=11896166 DB - PRIME DP - Unbound Medicine ER -