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Endogenous mast cell degranulation modulates cervical contractility in the guinea pig.
Am J Obstet Gynecol. 2002 Mar; 186(3):438-45.AJ

Abstract

OBJECTIVE

The purpose of this study was to investigate the effect of endogenous mast cell degranulation on the contractility of isolated cervical strips from nonpregnant and pregnant guinea pigs.

STUDY DESIGN

Longitudinal cervical strips from nonpregnant and pregnant (mid and term) guinea pigs were used for isometric tension recording. Responses to the mast cell degranulating agent, compound 48/80, were compared in the absence or presence of different inhibitors and receptor antagonists. Concentration-response curves were obtained to histamine and 5-hydroxytryptamine in strips that were incubated with antagonists or solvent.

RESULTS

Compound 48/80 and histamine significantly increased contractility of cervical strips in all 3 groups of animals. The inhibitor of mast cell degranulation significantly reduced responses to compound 48/80 and histamine-1 receptor antagonist reduced responses to histamine in all 3 groups. Histamine-1 receptor antagonist significantly inhibited responses to compound 48/80 in nonpregnant and mid pregnant guinea pigs. Histamine-2 receptor antagonist did not alter responses to compound 48/80 nor to histamine. The receptor antagonist 5-hydroxytryptamine-2 significantly inhibited cervical contractility that was induced by compound 48/80 in tissues from mid pregnant and term pregnant guinea pigs. Lipoxygenase inhibitor was effective in mid pregnant guinea pigs. Cyclooxygenase inhibitor, 5-hydroxytryptamine, and a combination of lipoxygenase and cyclooxygenase inhibitors had no effect on cervical contractility.

CONCLUSION

The degranulation of mast cells releases histamine and other mediators that stimulate cervical contractility through histamine-1 receptors. Cervical infiltration and modulation of contractility by mast cells may play an important physiologic and/or pathologic role in the control of cervical function during pregnancy.

Authors+Show Affiliations

Department of Obstetrics & Gynecology, Division of Reproductive Sciences, The University of Texas Medical Branch, Galveston 77555-1062, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11904604

Citation

Bytautiene, Egle, et al. "Endogenous Mast Cell Degranulation Modulates Cervical Contractility in the Guinea Pig." American Journal of Obstetrics and Gynecology, vol. 186, no. 3, 2002, pp. 438-45.
Bytautiene E, Vedernikov YP, Saade GR, et al. Endogenous mast cell degranulation modulates cervical contractility in the guinea pig. Am J Obstet Gynecol. 2002;186(3):438-45.
Bytautiene, E., Vedernikov, Y. P., Saade, G. R., Romero, R., & Garfield, R. E. (2002). Endogenous mast cell degranulation modulates cervical contractility in the guinea pig. American Journal of Obstetrics and Gynecology, 186(3), 438-45.
Bytautiene E, et al. Endogenous Mast Cell Degranulation Modulates Cervical Contractility in the Guinea Pig. Am J Obstet Gynecol. 2002;186(3):438-45. PubMed PMID: 11904604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endogenous mast cell degranulation modulates cervical contractility in the guinea pig. AU - Bytautiene,Egle, AU - Vedernikov,Yuri P, AU - Saade,George R, AU - Romero,Roberto, AU - Garfield,Robert E, PY - 2002/3/21/pubmed PY - 2002/4/16/medline PY - 2002/3/21/entrez SP - 438 EP - 45 JF - American journal of obstetrics and gynecology JO - Am J Obstet Gynecol VL - 186 IS - 3 N2 - OBJECTIVE: The purpose of this study was to investigate the effect of endogenous mast cell degranulation on the contractility of isolated cervical strips from nonpregnant and pregnant guinea pigs. STUDY DESIGN: Longitudinal cervical strips from nonpregnant and pregnant (mid and term) guinea pigs were used for isometric tension recording. Responses to the mast cell degranulating agent, compound 48/80, were compared in the absence or presence of different inhibitors and receptor antagonists. Concentration-response curves were obtained to histamine and 5-hydroxytryptamine in strips that were incubated with antagonists or solvent. RESULTS: Compound 48/80 and histamine significantly increased contractility of cervical strips in all 3 groups of animals. The inhibitor of mast cell degranulation significantly reduced responses to compound 48/80 and histamine-1 receptor antagonist reduced responses to histamine in all 3 groups. Histamine-1 receptor antagonist significantly inhibited responses to compound 48/80 in nonpregnant and mid pregnant guinea pigs. Histamine-2 receptor antagonist did not alter responses to compound 48/80 nor to histamine. The receptor antagonist 5-hydroxytryptamine-2 significantly inhibited cervical contractility that was induced by compound 48/80 in tissues from mid pregnant and term pregnant guinea pigs. Lipoxygenase inhibitor was effective in mid pregnant guinea pigs. Cyclooxygenase inhibitor, 5-hydroxytryptamine, and a combination of lipoxygenase and cyclooxygenase inhibitors had no effect on cervical contractility. CONCLUSION: The degranulation of mast cells releases histamine and other mediators that stimulate cervical contractility through histamine-1 receptors. Cervical infiltration and modulation of contractility by mast cells may play an important physiologic and/or pathologic role in the control of cervical function during pregnancy. SN - 0002-9378 UR - https://www.unboundmedicine.com/medline/citation/11904604/Endogenous_mast_cell_degranulation_modulates_cervical_contractility_in_the_guinea_pig_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S000293780247665X DB - PRIME DP - Unbound Medicine ER -