Tags

Type your tag names separated by a space and hit enter

Cellular defense against UVB-induced phototoxicity by cytosolic NADP(+)-dependent isocitrate dehydrogenase.
Biochem Biophys Res Commun. 2002 Mar 29; 292(2):542-9.BB

Abstract

Ultraviolet (UV) radiation is known as a major cause of skin photoaging and photocarcinogenesis. Many harmful effects of UV radiation are associated with the generation of reactive oxygen species. Recently, we have shown that NADP(+)-dependent isocitrate dehydrogenase is involved in the supply of NADPH needed for GSH production against cellular oxidative damage. In this study we investigated the role of cytosolic form of NADP(+)-dependent isocitrate dehydrogenase (IDPc) against UV radiation-induced cytotoxicity by comparing the relative degree of cellular responses in three different NIH3T3 cells with stable transfection with the cDNA for mouse IDPc in sense and antisense orientations, where IDPc activities were 2.3-fold higher and 39% lower, respectively, than that in the parental cells carrying the vector alone. Upon exposure to UVB (312 nm), the cells with low levels of IDPc became more sensitive to cell killing. Lipid peroxidation, protein oxidation, oxidative DNA damage, and intracellular peroxide generation were higher in the cell-line expressing the lower level of IDPc. However, the cells with the highly overexpressed IDPc exhibited enhanced resistance against UV radiation, compared to the control cells. The data indicate that IDPc plays an important role in cellular defense against UV radiation-induced oxidative injury.

Authors+Show Affiliations

Department of Genetic Engineering, Kyungpook National University, Daegu, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11906195

Citation

Jo, Seung-Hee, et al. "Cellular Defense Against UVB-induced Phototoxicity By Cytosolic NADP(+)-dependent Isocitrate Dehydrogenase." Biochemical and Biophysical Research Communications, vol. 292, no. 2, 2002, pp. 542-9.
Jo SH, Lee SH, Chun HS, et al. Cellular defense against UVB-induced phototoxicity by cytosolic NADP(+)-dependent isocitrate dehydrogenase. Biochem Biophys Res Commun. 2002;292(2):542-9.
Jo, S. H., Lee, S. H., Chun, H. S., Lee, S. M., Koh, H. J., Lee, S. E., Chun, J. S., Park, J. W., & Huh, T. L. (2002). Cellular defense against UVB-induced phototoxicity by cytosolic NADP(+)-dependent isocitrate dehydrogenase. Biochemical and Biophysical Research Communications, 292(2), 542-9.
Jo SH, et al. Cellular Defense Against UVB-induced Phototoxicity By Cytosolic NADP(+)-dependent Isocitrate Dehydrogenase. Biochem Biophys Res Commun. 2002 Mar 29;292(2):542-9. PubMed PMID: 11906195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular defense against UVB-induced phototoxicity by cytosolic NADP(+)-dependent isocitrate dehydrogenase. AU - Jo,Seung-Hee, AU - Lee,So-Hyun, AU - Chun,Hang Suk, AU - Lee,Su Min, AU - Koh,Ho-Jin, AU - Lee,Sung-Eun, AU - Chun,Jang-Soo, AU - Park,Jeen-Woo, AU - Huh,Tae-Lin, PY - 2002/3/22/pubmed PY - 2002/5/7/medline PY - 2002/3/22/entrez SP - 542 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 292 IS - 2 N2 - Ultraviolet (UV) radiation is known as a major cause of skin photoaging and photocarcinogenesis. Many harmful effects of UV radiation are associated with the generation of reactive oxygen species. Recently, we have shown that NADP(+)-dependent isocitrate dehydrogenase is involved in the supply of NADPH needed for GSH production against cellular oxidative damage. In this study we investigated the role of cytosolic form of NADP(+)-dependent isocitrate dehydrogenase (IDPc) against UV radiation-induced cytotoxicity by comparing the relative degree of cellular responses in three different NIH3T3 cells with stable transfection with the cDNA for mouse IDPc in sense and antisense orientations, where IDPc activities were 2.3-fold higher and 39% lower, respectively, than that in the parental cells carrying the vector alone. Upon exposure to UVB (312 nm), the cells with low levels of IDPc became more sensitive to cell killing. Lipid peroxidation, protein oxidation, oxidative DNA damage, and intracellular peroxide generation were higher in the cell-line expressing the lower level of IDPc. However, the cells with the highly overexpressed IDPc exhibited enhanced resistance against UV radiation, compared to the control cells. The data indicate that IDPc plays an important role in cellular defense against UV radiation-induced oxidative injury. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/11906195/Cellular_defense_against_UVB_induced_phototoxicity_by_cytosolic_NADP_+__dependent_isocitrate_dehydrogenase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006291X02966672 DB - PRIME DP - Unbound Medicine ER -