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Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents.
J Virol Methods. 2002 May; 103(1):57-66.JV

Abstract

Junin virus is the etiological agent of Argentine hemorrhagic fever, a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys musculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation. A high correlation (K=0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and murine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples.

Authors+Show Affiliations

Instituto Nacional de Enfermedades Virales Humanas Dr. Julio I. Maiztegui, Administración Nacional de Laboratorios e Institutos de Salud, Monteagudo 2510, (2700) Pergamino, Buenos Aires, Argentina. morales_ma@operamail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

11906733

Citation

Morales, María A., et al. "Evaluation of an Enzyme-linked Immunosorbent Assay for Detection of Antibodies to Junin Virus in Rodents." Journal of Virological Methods, vol. 103, no. 1, 2002, pp. 57-66.
Morales MA, Calderón GE, Riera LM, et al. Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents. J Virol Methods. 2002;103(1):57-66.
Morales, M. A., Calderón, G. E., Riera, L. M., Ambrosio, A. M., Enría, D. A., & Sabattini, M. S. (2002). Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents. Journal of Virological Methods, 103(1), 57-66.
Morales MA, et al. Evaluation of an Enzyme-linked Immunosorbent Assay for Detection of Antibodies to Junin Virus in Rodents. J Virol Methods. 2002;103(1):57-66. PubMed PMID: 11906733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents. AU - Morales,María A, AU - Calderón,Gladys E, AU - Riera,Laura M, AU - Ambrosio,Ana M, AU - Enría,Delia A, AU - Sabattini,Marta S, PY - 2002/3/22/pubmed PY - 2002/7/23/medline PY - 2002/3/22/entrez SP - 57 EP - 66 JF - Journal of virological methods JO - J Virol Methods VL - 103 IS - 1 N2 - Junin virus is the etiological agent of Argentine hemorrhagic fever, a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys musculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation. A high correlation (K=0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and murine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/11906733/Evaluation_of_an_enzyme_linked_immunosorbent_assay_for_detection_of_antibodies_to_Junin_virus_in_rodents_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166093401004529 DB - PRIME DP - Unbound Medicine ER -