Tags

Type your tag names separated by a space and hit enter

Expression of estrogen receptors alpha and beta in the baboon fetal ovary.
Biol Reprod. 2002 Apr; 66(4):1054-60.BR

Abstract

In adult mammals, estrogen regulates ovarian function, and estrogen receptor (ER) is expressed in granulosa cells of antral follicles of the adult baboon ovary. Because the foundation of adult ovarian function is established in utero, the present study determined whether ERalpha and/or ERbeta were expressed in fetal ovaries obtained on Days 100 (n = 3) and 165-181 (n = 5) of baboon gestation (term = Day 184). On Day 100, ERalpha protein was detected by immunocytochemistry in surface epithelium and mesenchymal-epithelial cells but not oocytes in germ cell cords. ERbeta protein was also detected by immunocytochemistry on Day 100 of gestation and was abundantly expressed in mesenchymal-epithelial cells in germ cell cords, lightly expressed in the germ cells, but was not detected in the surface epithelium. On Days 165-180 of gestation, ERalpha expression was still intense in the surface epithelium, in mesenchymal-epithelial cells throughout the cortex, and in nests of cells between follicles. ERalpha expression was lighter in granulosa cells and was not observed in all granulosa cells, particularly in follicles close to the cortex. In contrast, ERbeta expression was most intense in granulosa cells, especially in flattened granulosa cells, was weaker in mesenchymal-epithelial cells and nests of cells between follicles, and was absent in the surface epithelium. Using an antibody to the carboxy terminal of human ERbeta, ERbeta protein was also detected by Western immunoblot with molecular sizes of 55 and 63 kDa on Day 100 and primarily 55 kDa on Day 180. The mRNAs for ERalpha and ERbeta were also detected by Northern blot analysis in the baboon fetal ovary. These results are the first to establish that the ERalpha and ERbeta mRNAs and proteins are expressed and exhibit changes in localization in the primate fetal ovary between mid and late gestation. Because placental estrogen production and secretion into the baboon fetus increases markedly during advancing pregnancy, we propose that estrogen plays an integral role in programming fetal ovarian development in the primate.

Authors+Show Affiliations

Department of Physiological Sciences, Eastern Virginia Medical School, 700 Olney Road, Norfolk, VA 23501-1980, USA. pepegj@evms.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11906925

Citation

Pepe, Gerald J., et al. "Expression of Estrogen Receptors Alpha and Beta in the Baboon Fetal Ovary." Biology of Reproduction, vol. 66, no. 4, 2002, pp. 1054-60.
Pepe GJ, Billiar RB, Leavitt MG, et al. Expression of estrogen receptors alpha and beta in the baboon fetal ovary. Biol Reprod. 2002;66(4):1054-60.
Pepe, G. J., Billiar, R. B., Leavitt, M. G., Zachos, N. C., Gustafsson, J. A., & Albrecht, E. D. (2002). Expression of estrogen receptors alpha and beta in the baboon fetal ovary. Biology of Reproduction, 66(4), 1054-60.
Pepe GJ, et al. Expression of Estrogen Receptors Alpha and Beta in the Baboon Fetal Ovary. Biol Reprod. 2002;66(4):1054-60. PubMed PMID: 11906925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of estrogen receptors alpha and beta in the baboon fetal ovary. AU - Pepe,Gerald J, AU - Billiar,Reinhart B, AU - Leavitt,Maria G, AU - Zachos,Nicholas C, AU - Gustafsson,Jan Ake, AU - Albrecht,Eugene D, PY - 2002/3/22/pubmed PY - 2002/7/2/medline PY - 2002/3/22/entrez SP - 1054 EP - 60 JF - Biology of reproduction JO - Biol Reprod VL - 66 IS - 4 N2 - In adult mammals, estrogen regulates ovarian function, and estrogen receptor (ER) is expressed in granulosa cells of antral follicles of the adult baboon ovary. Because the foundation of adult ovarian function is established in utero, the present study determined whether ERalpha and/or ERbeta were expressed in fetal ovaries obtained on Days 100 (n = 3) and 165-181 (n = 5) of baboon gestation (term = Day 184). On Day 100, ERalpha protein was detected by immunocytochemistry in surface epithelium and mesenchymal-epithelial cells but not oocytes in germ cell cords. ERbeta protein was also detected by immunocytochemistry on Day 100 of gestation and was abundantly expressed in mesenchymal-epithelial cells in germ cell cords, lightly expressed in the germ cells, but was not detected in the surface epithelium. On Days 165-180 of gestation, ERalpha expression was still intense in the surface epithelium, in mesenchymal-epithelial cells throughout the cortex, and in nests of cells between follicles. ERalpha expression was lighter in granulosa cells and was not observed in all granulosa cells, particularly in follicles close to the cortex. In contrast, ERbeta expression was most intense in granulosa cells, especially in flattened granulosa cells, was weaker in mesenchymal-epithelial cells and nests of cells between follicles, and was absent in the surface epithelium. Using an antibody to the carboxy terminal of human ERbeta, ERbeta protein was also detected by Western immunoblot with molecular sizes of 55 and 63 kDa on Day 100 and primarily 55 kDa on Day 180. The mRNAs for ERalpha and ERbeta were also detected by Northern blot analysis in the baboon fetal ovary. These results are the first to establish that the ERalpha and ERbeta mRNAs and proteins are expressed and exhibit changes in localization in the primate fetal ovary between mid and late gestation. Because placental estrogen production and secretion into the baboon fetus increases markedly during advancing pregnancy, we propose that estrogen plays an integral role in programming fetal ovarian development in the primate. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/11906925/Expression_of_estrogen_receptors_alpha_and_beta_in_the_baboon_fetal_ovary_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod66.4.1054 DB - PRIME DP - Unbound Medicine ER -