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Rapid verification of wheat-Hordeum introgressions by direct staining of SCAR, STS, and SSR amplicons.
Genome. 2002 Feb; 45(1):198-203.G

Abstract

A range of single tagged site (STS), simple sequence repeat (SSR), and sequence-characterized amplified region (SCAR) markers were screened for their utility in detecting Hordeum vulgare and H. chilense chromosomes in a wheat background. PCR conditions were optimized for specific amplification of the targeted sequences and to avoid cross-species amplification. Two H. vulgare derived STSs, six H. vulgare derived SSRs, and nine H. chilense derived SCARs were usable for the detection of five H. vulgare and three H. chilense chromosomes by direct ethidium bromide staining of the PCR products in test tubes, avoiding the more costly and time-consuming DNA electrophoresis step. The practical application of the method is illustrated by the identification of a monotelosomic substitution of H. vulgare chromosome 6HS in tritordeum and a monosomic addition of H. chilense chromosome 6Hch in durum wheat.

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Authors+Show Affiliations

Hernández P
Instituto de Agricultura Sostenible (CSIC), Córdoba, Spain. ge1hemop@uco.es
Dorado G
No affiliation info available
Cabrera A
No affiliation info available
Laurie DA
No affiliation info available
Snape JW
No affiliation info available
Martín A
No affiliation info available

MeSH

Genetic MarkersHordeumPolymerase Chain ReactionRepetitive Sequences, Nucleic AcidTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11908662

Citation

Hernández, P, et al. "Rapid Verification of wheat-Hordeum Introgressions By Direct Staining of SCAR, STS, and SSR Amplicons." Genome, vol. 45, no. 1, 2002, pp. 198-203.
Hernández P, Dorado G, Cabrera A, et al. Rapid verification of wheat-Hordeum introgressions by direct staining of SCAR, STS, and SSR amplicons. Genome. 2002;45(1):198-203.
Hernández, P., Dorado, G., Cabrera, A., Laurie, D. A., Snape, J. W., & Martín, A. (2002). Rapid verification of wheat-Hordeum introgressions by direct staining of SCAR, STS, and SSR amplicons. Genome, 45(1), 198-203.
Hernández P, et al. Rapid Verification of wheat-Hordeum Introgressions By Direct Staining of SCAR, STS, and SSR Amplicons. Genome. 2002;45(1):198-203. PubMed PMID: 11908662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid verification of wheat-Hordeum introgressions by direct staining of SCAR, STS, and SSR amplicons. AU - Hernández,P, AU - Dorado,G, AU - Cabrera,A, AU - Laurie,D A, AU - Snape,J W, AU - Martín,A, PY - 2002/3/23/pubmed PY - 2002/10/12/medline PY - 2002/3/23/entrez SP - 198 EP - 203 JF - Genome JO - Genome VL - 45 IS - 1 N2 - A range of single tagged site (STS), simple sequence repeat (SSR), and sequence-characterized amplified region (SCAR) markers were screened for their utility in detecting Hordeum vulgare and H. chilense chromosomes in a wheat background. PCR conditions were optimized for specific amplification of the targeted sequences and to avoid cross-species amplification. Two H. vulgare derived STSs, six H. vulgare derived SSRs, and nine H. chilense derived SCARs were usable for the detection of five H. vulgare and three H. chilense chromosomes by direct ethidium bromide staining of the PCR products in test tubes, avoiding the more costly and time-consuming DNA electrophoresis step. The practical application of the method is illustrated by the identification of a monotelosomic substitution of H. vulgare chromosome 6HS in tritordeum and a monosomic addition of H. chilense chromosome 6Hch in durum wheat. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/11908662/Rapid_verification_of_wheat_Hordeum_introgressions_by_direct_staining_of_SCAR_STS_and_SSR_amplicons_ L2 - https://cdnsciencepub.com/doi/10.1139/g01-087?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -