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On-column derivatization for determination of amphetamine and methamphetamine in human blood by gas chromatography-mass spectrometry.
Forensic Sci Int. 2002 Feb 18; 125(2-3):156-62.FS

Abstract

A simple determination method of amphetamine (AP) and methamphetamine (MA) in human blood was developed using on-column derivatization and gas chromatography-mass spectrometry (GC-MS). AP and MA were adsorbed on the surface of Extrelut and then derivatized the N-propoxycarbonyl derivatives using propylchloroformate. Pentadeuterated MA was used as an internal standards. The recoveries of AP and MA from the spiked blood were 89.7 and 90.3%, respectively. The calibration curves showed linearity in the range of 12.5-2000 ng/g for AP and MA in blood. The coefficients of variation of intraday and interday were 0.42-4.58%. Furthermore, this proposed method was applied to some medico-legal cases of MA intoxication. MA and its metabolite AP were detected in the blood samples, and the correlation of the blood level of amphetamines and the behaviors of the victims was in good agreement with the criteria proposed by Nagata [Jpn. J. Legal Med. 37 (1983) 513].

Authors+Show Affiliations

Department of Legal Medicine, Faculty of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8551, Japan. nishidam@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11909658

Citation

Nishida, Manami, et al. "On-column Derivatization for Determination of Amphetamine and Methamphetamine in Human Blood By Gas Chromatography-mass Spectrometry." Forensic Science International, vol. 125, no. 2-3, 2002, pp. 156-62.
Nishida M, Namera A, Yashiki M, et al. On-column derivatization for determination of amphetamine and methamphetamine in human blood by gas chromatography-mass spectrometry. Forensic Sci Int. 2002;125(2-3):156-62.
Nishida, M., Namera, A., Yashiki, M., & Kojima, T. (2002). On-column derivatization for determination of amphetamine and methamphetamine in human blood by gas chromatography-mass spectrometry. Forensic Science International, 125(2-3), 156-62.
Nishida M, et al. On-column Derivatization for Determination of Amphetamine and Methamphetamine in Human Blood By Gas Chromatography-mass Spectrometry. Forensic Sci Int. 2002 Feb 18;125(2-3):156-62. PubMed PMID: 11909658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-column derivatization for determination of amphetamine and methamphetamine in human blood by gas chromatography-mass spectrometry. AU - Nishida,Manami, AU - Namera,Akira, AU - Yashiki,Mikio, AU - Kojima,Tohru, PY - 2002/3/23/pubmed PY - 2002/6/5/medline PY - 2002/3/23/entrez SP - 156 EP - 62 JF - Forensic science international JO - Forensic Sci. Int. VL - 125 IS - 2-3 N2 - A simple determination method of amphetamine (AP) and methamphetamine (MA) in human blood was developed using on-column derivatization and gas chromatography-mass spectrometry (GC-MS). AP and MA were adsorbed on the surface of Extrelut and then derivatized the N-propoxycarbonyl derivatives using propylchloroformate. Pentadeuterated MA was used as an internal standards. The recoveries of AP and MA from the spiked blood were 89.7 and 90.3%, respectively. The calibration curves showed linearity in the range of 12.5-2000 ng/g for AP and MA in blood. The coefficients of variation of intraday and interday were 0.42-4.58%. Furthermore, this proposed method was applied to some medico-legal cases of MA intoxication. MA and its metabolite AP were detected in the blood samples, and the correlation of the blood level of amphetamines and the behaviors of the victims was in good agreement with the criteria proposed by Nagata [Jpn. J. Legal Med. 37 (1983) 513]. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/11909658/On_column_derivatization_for_determination_of_amphetamine_and_methamphetamine_in_human_blood_by_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379073801006314 DB - PRIME DP - Unbound Medicine ER -