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Regulation of calbindin-D9k expression by 1,25-dihydroxyvitamin D(3) and parathyroid hormone in mouse primary renal tubular cells.
Arch Biochem Biophys 2002; 400(1):118-24AB

Abstract

Calbindin (CaBP)-D9k is a major vitamin D target gene involved in calcium homeostasis. However, studies on the molecular mechanisms of CaBP-D9k gene regulation have been hampered by the lack of an appropriate cell culture system. In the present study, we used mouse primary renal tubular cell (PRTC) cultures to investigate the regulation of CaBP-D9k expression by 1,25(OH)(2)D(3). Both CaBP-D9k mRNA and protein were highly induced by 1,25(OH)(2)D(3) in a time- and dose-dependent manner in PRTCs, and new RNA and protein synthesis was required for the induction. Transfection of VDR(-/-) cells derived from VDR null mice with human VDR restored the induction of CaBP-D9k expression by 1,25(OH)(2)D(3), confirming the requirement of VDR for CaBP-D9k expression. Treatment of the PRTCs with 1,25(OH)(2)D(3) also increased VDR protein abundance, suggesting that enhanced VDR transactivation is involved in the CaBP-D9k up-regulation. Moreover, PTH had a synergistic effect on the 1,25(OH)(2)D(3) induction of CaBP-D9k. These data demonstrate that CaBP-D9k is highly regulated by 1,25(OH)(2)D(3) and PTH in mouse PRTCs, which provides a suitable in vitro system for further investigating the molecular mechanisms involved in CaBP-D9k gene regulation.

Authors+Show Affiliations

Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11913978

Citation

Cao, Li-Ping, et al. "Regulation of calbindin-D9k Expression By 1,25-dihydroxyvitamin D(3) and Parathyroid Hormone in Mouse Primary Renal Tubular Cells." Archives of Biochemistry and Biophysics, vol. 400, no. 1, 2002, pp. 118-24.
Cao LP, Bolt MJ, Wei M, et al. Regulation of calbindin-D9k expression by 1,25-dihydroxyvitamin D(3) and parathyroid hormone in mouse primary renal tubular cells. Arch Biochem Biophys. 2002;400(1):118-24.
Cao, L. P., Bolt, M. J., Wei, M., Sitrin, M. D., & Chun Li, Y. (2002). Regulation of calbindin-D9k expression by 1,25-dihydroxyvitamin D(3) and parathyroid hormone in mouse primary renal tubular cells. Archives of Biochemistry and Biophysics, 400(1), pp. 118-24.
Cao LP, et al. Regulation of calbindin-D9k Expression By 1,25-dihydroxyvitamin D(3) and Parathyroid Hormone in Mouse Primary Renal Tubular Cells. Arch Biochem Biophys. 2002 Apr 1;400(1):118-24. PubMed PMID: 11913978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of calbindin-D9k expression by 1,25-dihydroxyvitamin D(3) and parathyroid hormone in mouse primary renal tubular cells. AU - Cao,Li-Ping, AU - Bolt,Merry J G, AU - Wei,Minjie, AU - Sitrin,Michael D, AU - Chun Li,Yan, PY - 2002/3/27/pubmed PY - 2002/5/7/medline PY - 2002/3/27/entrez SP - 118 EP - 24 JF - Archives of biochemistry and biophysics JO - Arch. Biochem. Biophys. VL - 400 IS - 1 N2 - Calbindin (CaBP)-D9k is a major vitamin D target gene involved in calcium homeostasis. However, studies on the molecular mechanisms of CaBP-D9k gene regulation have been hampered by the lack of an appropriate cell culture system. In the present study, we used mouse primary renal tubular cell (PRTC) cultures to investigate the regulation of CaBP-D9k expression by 1,25(OH)(2)D(3). Both CaBP-D9k mRNA and protein were highly induced by 1,25(OH)(2)D(3) in a time- and dose-dependent manner in PRTCs, and new RNA and protein synthesis was required for the induction. Transfection of VDR(-/-) cells derived from VDR null mice with human VDR restored the induction of CaBP-D9k expression by 1,25(OH)(2)D(3), confirming the requirement of VDR for CaBP-D9k expression. Treatment of the PRTCs with 1,25(OH)(2)D(3) also increased VDR protein abundance, suggesting that enhanced VDR transactivation is involved in the CaBP-D9k up-regulation. Moreover, PTH had a synergistic effect on the 1,25(OH)(2)D(3) induction of CaBP-D9k. These data demonstrate that CaBP-D9k is highly regulated by 1,25(OH)(2)D(3) and PTH in mouse PRTCs, which provides a suitable in vitro system for further investigating the molecular mechanisms involved in CaBP-D9k gene regulation. SN - 0003-9861 UR - https://www.unboundmedicine.com/medline/citation/11913978/Regulation_of_calbindin_D9k_expression_by_125_dihydroxyvitamin_D_3__and_parathyroid_hormone_in_mouse_primary_renal_tubular_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S000398610292775X DB - PRIME DP - Unbound Medicine ER -