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The changes of IGF binding proteins after rhGH administration to patients totally dependent on parenteral nutrition.
Growth Horm IGF Res. 2001 Dec; 11(6):407-15.GH

Abstract

The objective was to study the effect of recombinant human growth hormone (rhGH) administration to patients with chronic malnutrition maintained on total parenteral nutrition (TPN) on the levels of insulin-like growth factor-I (IGF-I) and IGF binding proteins (IGFBPs) during a double-blind trial. After 1 week of TPN the patients were randomized into group I (placebo) or group II (rhGH). Samples were collected on the first day (start of the TPN) to measure basal values, the seventh day to study the effect of TPN and the 10th, 14th and 21st days to evaluate the rhGH effect. Basal laboratory evaluation, nutritional status and glucose tolerance were assessed using standard laboratory techniques. Radioimmunoassays were used to analyse IGF-I, free IGF-I (fIGF-I) and IGFBP1-3. Electrophoresis with Western ligand blotting and Western immunoblotting was applied to find the pattern of IGFBPs. TPN had no effect on the circulating IGF-I concentration and the pattern of IGFBPs present in the studied groups of patients. The rhGH administration led to significant increases of IGF-I, total IGFBP-3, glycosylated IGFBP-3 (39, 42 kDa) and the 29 kDa fragment of IGFBP-3 and the decrease of IGFBP-2 during the trial (P<0.05). The mean levels of IGFBP-1, fIGF-I and the parameters of nutritional status in group II during the trial were not significantly influenced by rhGH. However, it has been found that IGFBP-1 and fIGF-I levels were correlated with the levels of the weekly nitrogen balance of each patient in group II at the end of the trial. In spite of the significant changes of IGF-I, IGFBP-2, total IGFBP-3 and IGFBP-3 (29 kDa proteolytic fragment) after rhGH administration to patients with malnutrition, maintained on parenteral nutrition, the increase of nitrogen balance was seen only in patients who decreased their IGFBP-1 and increased bioavailable IGF-I as reflected by measurement of fIGF-I. The levels of IGFBP-1 may provide a useful marker of IGF-I bioavailability in monitoring the efficiency of the rhGH therapy in malnourished patients.

Authors+Show Affiliations

Third Medical Department of Charles University, Prague, Czech Republic. vjustova@vfn.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11914029

Citation

Justová, V, et al. "The Changes of IGF Binding Proteins After rhGH Administration to Patients Totally Dependent On Parenteral Nutrition." Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society, vol. 11, no. 6, 2001, pp. 407-15.
Justová V, Lacinová Z, Melenovský V, et al. The changes of IGF binding proteins after rhGH administration to patients totally dependent on parenteral nutrition. Growth Horm IGF Res. 2001;11(6):407-15.
Justová, V., Lacinová, Z., Melenovský, V., Marek, J., Holly, J. M., & Hass, T. (2001). The changes of IGF binding proteins after rhGH administration to patients totally dependent on parenteral nutrition. Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society, 11(6), 407-15.
Justová V, et al. The Changes of IGF Binding Proteins After rhGH Administration to Patients Totally Dependent On Parenteral Nutrition. Growth Horm IGF Res. 2001;11(6):407-15. PubMed PMID: 11914029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The changes of IGF binding proteins after rhGH administration to patients totally dependent on parenteral nutrition. AU - Justová,V, AU - Lacinová,Z, AU - Melenovský,V, AU - Marek,J, AU - Holly,J M, AU - Hass,T, PY - 2002/3/27/pubmed PY - 2002/6/4/medline PY - 2002/3/27/entrez SP - 407 EP - 15 JF - Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society JO - Growth Horm. IGF Res. VL - 11 IS - 6 N2 - The objective was to study the effect of recombinant human growth hormone (rhGH) administration to patients with chronic malnutrition maintained on total parenteral nutrition (TPN) on the levels of insulin-like growth factor-I (IGF-I) and IGF binding proteins (IGFBPs) during a double-blind trial. After 1 week of TPN the patients were randomized into group I (placebo) or group II (rhGH). Samples were collected on the first day (start of the TPN) to measure basal values, the seventh day to study the effect of TPN and the 10th, 14th and 21st days to evaluate the rhGH effect. Basal laboratory evaluation, nutritional status and glucose tolerance were assessed using standard laboratory techniques. Radioimmunoassays were used to analyse IGF-I, free IGF-I (fIGF-I) and IGFBP1-3. Electrophoresis with Western ligand blotting and Western immunoblotting was applied to find the pattern of IGFBPs. TPN had no effect on the circulating IGF-I concentration and the pattern of IGFBPs present in the studied groups of patients. The rhGH administration led to significant increases of IGF-I, total IGFBP-3, glycosylated IGFBP-3 (39, 42 kDa) and the 29 kDa fragment of IGFBP-3 and the decrease of IGFBP-2 during the trial (P<0.05). The mean levels of IGFBP-1, fIGF-I and the parameters of nutritional status in group II during the trial were not significantly influenced by rhGH. However, it has been found that IGFBP-1 and fIGF-I levels were correlated with the levels of the weekly nitrogen balance of each patient in group II at the end of the trial. In spite of the significant changes of IGF-I, IGFBP-2, total IGFBP-3 and IGFBP-3 (29 kDa proteolytic fragment) after rhGH administration to patients with malnutrition, maintained on parenteral nutrition, the increase of nitrogen balance was seen only in patients who decreased their IGFBP-1 and increased bioavailable IGF-I as reflected by measurement of fIGF-I. The levels of IGFBP-1 may provide a useful marker of IGF-I bioavailability in monitoring the efficiency of the rhGH therapy in malnourished patients. SN - 1096-6374 UR - https://www.unboundmedicine.com/medline/citation/11914029/The_changes_of_IGF_binding_proteins_after_rhGH_administration_to_patients_totally_dependent_on_parenteral_nutrition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-6374(01)90257-6 DB - PRIME DP - Unbound Medicine ER -