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Galactose-1-phosphate uridyltransferase in cultured cells.
Clin Chim Acta. 1976 Dec; 73(3):537-46.CC

Abstract

A method to measure the enzyme galactose-1-phosphate uridyltransferase in cultured cells is described. The optimun pH was 8.7 and no enzyme activity was found without preincubation with dithiothreitol. The KM values for Gal-1-P and UDPG for the wild type enzyme were found to be 0.2 mM and 0.08 mM, respectively. Values for the Duarte variant were found to be identical. No significant change in enzyme activity with time after subculture was found in either cultured skin fibroblasts or cultured amniotic fluid cells. Different transferase genotypes were clearly distinguished and reference range established. Transferase levels found in normal cultured amniotic fluid cells were the same as in normal cultured skin fibroblasts. The results of a prenatal diagnosis was obtained within 3 weeks of amniocentesis.

Authors

Monk AM
No affiliation info available
Holton JB
No affiliation info available

MeSH

AmniocentesisAmniotic FluidCell DivisionCells, CulturedFemaleGalactosemiasGenetic VariationGenotypeHumansHydrogen-Ion ConcentrationKineticsMaleNucleotidyltransferasesPregnancyPrenatal DiagnosisSkinUDPglucose-Hexose-1-Phosphate Uridylyltransferase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11916

Citation

Monk, A M., and J B. Holton. "Galactose-1-phosphate Uridyltransferase in Cultured Cells." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 73, no. 3, 1976, pp. 537-46.
Monk AM, Holton JB. Galactose-1-phosphate uridyltransferase in cultured cells. Clin Chim Acta. 1976;73(3):537-46.
Monk, A. M., & Holton, J. B. (1976). Galactose-1-phosphate uridyltransferase in cultured cells. Clinica Chimica Acta; International Journal of Clinical Chemistry, 73(3), 537-46.
Monk AM, Holton JB. Galactose-1-phosphate Uridyltransferase in Cultured Cells. Clin Chim Acta. 1976;73(3):537-46. PubMed PMID: 11916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Galactose-1-phosphate uridyltransferase in cultured cells. AU - Monk,A M, AU - Holton,J B, PY - 1976/12/1/pubmed PY - 1976/12/1/medline PY - 1976/12/1/entrez SP - 537 EP - 46 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 73 IS - 3 N2 - A method to measure the enzyme galactose-1-phosphate uridyltransferase in cultured cells is described. The optimun pH was 8.7 and no enzyme activity was found without preincubation with dithiothreitol. The KM values for Gal-1-P and UDPG for the wild type enzyme were found to be 0.2 mM and 0.08 mM, respectively. Values for the Duarte variant were found to be identical. No significant change in enzyme activity with time after subculture was found in either cultured skin fibroblasts or cultured amniotic fluid cells. Different transferase genotypes were clearly distinguished and reference range established. Transferase levels found in normal cultured amniotic fluid cells were the same as in normal cultured skin fibroblasts. The results of a prenatal diagnosis was obtained within 3 weeks of amniocentesis. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/11916/Galactose_1_phosphate_uridyltransferase_in_cultured_cells_ DB - PRIME DP - Unbound Medicine ER -