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The utility of activated partial thromboplastin time (aPTT) clot waveform analysis in the investigation of hemophilia A patients with very low levels of factor VIII activity (FVIII:C).
Thromb Haemost. 2002 Mar; 87(3):436-41.TH

Abstract

The lower detection limit of the conventional one-stage aPTT based clotting assay for determining FVIII:C levels is generally 1.0-2.0 IU/dl. Consequently, it has been impossible to study the clinical significance of levels of FVIII:C less than 1.0 IU/dl. Using a photo-optical automated coagulation analyzer, the Organon Teknika MDA II, we have performed qualitative and quantitative aPTT waveform analysis and measured FVIII:C levels by automated one-stage aPTT clotting assay in 36 severely affected Hemophilia A patients. Qualitative waveform analysis showed clear evidence of individual differences in the waveform profile suggesting differing coagulant activity from patient to patient. The FVIII:C level was less than 0.2 IU/dl in 23 cases and levels of FVIII:C between 0.2 and 1.0 IU/dl could be discriminated in 13 patients. The FVIII:C level in these patients was closely correlated with the minimum value of the second derivative of the aPTT waveform (Min2). This is a measure of the acceleration of change in optical transmission at the initiation of coagulation. Furthermore, the correlation of the

Authors+Show Affiliations

Department of Pediatrics, Nara Medical University, Kashihara City, Japan. mshima@naramed-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

11916076

Citation

Shima, Midori, et al. "The Utility of Activated Partial Thromboplastin Time (aPTT) Clot Waveform Analysis in the Investigation of Hemophilia a Patients With Very Low Levels of Factor VIII Activity (FVIII:C)." Thrombosis and Haemostasis, vol. 87, no. 3, 2002, pp. 436-41.
Shima M, Matsumoto T, Fukuda K, et al. The utility of activated partial thromboplastin time (aPTT) clot waveform analysis in the investigation of hemophilia A patients with very low levels of factor VIII activity (FVIII:C). Thromb Haemost. 2002;87(3):436-41.
Shima, M., Matsumoto, T., Fukuda, K., Kubota, Y., Tanaka, I., Nishiya, K., Giles, A. R., & Yoshioka, A. (2002). The utility of activated partial thromboplastin time (aPTT) clot waveform analysis in the investigation of hemophilia A patients with very low levels of factor VIII activity (FVIII:C). Thrombosis and Haemostasis, 87(3), 436-41.
Shima M, et al. The Utility of Activated Partial Thromboplastin Time (aPTT) Clot Waveform Analysis in the Investigation of Hemophilia a Patients With Very Low Levels of Factor VIII Activity (FVIII:C). Thromb Haemost. 2002;87(3):436-41. PubMed PMID: 11916076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The utility of activated partial thromboplastin time (aPTT) clot waveform analysis in the investigation of hemophilia A patients with very low levels of factor VIII activity (FVIII:C). AU - Shima,Midori, AU - Matsumoto,Tomoko, AU - Fukuda,Kazuyoshi, AU - Kubota,Youko, AU - Tanaka,Ichiro, AU - Nishiya,Katumi, AU - Giles,Alan R, AU - Yoshioka,Akira, PY - 2002/3/28/pubmed PY - 2003/8/26/medline PY - 2002/3/28/entrez SP - 436 EP - 41 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 87 IS - 3 N2 - The lower detection limit of the conventional one-stage aPTT based clotting assay for determining FVIII:C levels is generally 1.0-2.0 IU/dl. Consequently, it has been impossible to study the clinical significance of levels of FVIII:C less than 1.0 IU/dl. Using a photo-optical automated coagulation analyzer, the Organon Teknika MDA II, we have performed qualitative and quantitative aPTT waveform analysis and measured FVIII:C levels by automated one-stage aPTT clotting assay in 36 severely affected Hemophilia A patients. Qualitative waveform analysis showed clear evidence of individual differences in the waveform profile suggesting differing coagulant activity from patient to patient. The FVIII:C level was less than 0.2 IU/dl in 23 cases and levels of FVIII:C between 0.2 and 1.0 IU/dl could be discriminated in 13 patients. The FVIII:C level in these patients was closely correlated with the minimum value of the second derivative of the aPTT waveform (Min2). This is a measure of the acceleration of change in optical transmission at the initiation of coagulation. Furthermore, the correlation of the SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/11916076/The_utility_of_activated_partial_thromboplastin_time__aPTT__clot_waveform_analysis_in_the_investigation_of_hemophilia_A_patients_with_very_low_levels_of_factor_VIII_activity__FVIII:C__ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11916076.ui DB - PRIME DP - Unbound Medicine ER -