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Light- and electron-microscopic analysis of vasoactive intestinal polypeptide-immunoreactive amacrine cells in the guinea pig retina.
J Comp Neurol 2002; 445(4):325-35JC

Abstract

Vasoactive intestinal polypeptide (VIP) is a neuroactive substance that is expressed in both nonmammalian and mammalian retinas. This study investigated the morphology and synaptic connections of VIP-containing neurons in the guinea pig retina by immunocytochemistry, by using antisera against VIP. Specific VIP immunoreactivity was localized to a population of wide-field and regularly spaced amacrine cells with processes ramifying mainly in strata 1 and 2 of the inner plexiform layer (IPL). Double-label immunohistochemistry demonstrated that all VIP-immunoreactive cells possessed gamma-aminobutyric acid immunoreactivity. The synaptic connectivity of VIP-immunoreactive amacrine cells was identified in the IPL by electron microscopy. The VIP-labeled amacrine cell processes received synaptic input from other amacrine cell processes and bipolar cell axon terminals in strata 1 to 3 of the IPL. The most frequent postsynaptic targets of VIP-immunoreactive amacrine cells were other amacrine cell processes in strata 1 to 3 of the IPL. Synaptic outputs to bipolar cells were also observed in strata 1 to 3 of the IPL. In addition, ganglion cell dendrites were also postsynaptic to VIP-immunoreactive neurons in the sublamina a of the IPL. These studies show that one type of VIP-immunoreactive amacrine cells make contact predominantly with other amacrine cell processes. This finding suggests that VIP-containing amacrine cells may influence inner retinal circuitry, thus mediating visual processing.

Authors+Show Affiliations

Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11920710

Citation

Lee, Eun-Jin, et al. "Light- and Electron-microscopic Analysis of Vasoactive Intestinal Polypeptide-immunoreactive Amacrine Cells in the Guinea Pig Retina." The Journal of Comparative Neurology, vol. 445, no. 4, 2002, pp. 325-35.
Lee EJ, Park SH, Kim IB, et al. Light- and electron-microscopic analysis of vasoactive intestinal polypeptide-immunoreactive amacrine cells in the guinea pig retina. J Comp Neurol. 2002;445(4):325-35.
Lee, E. J., Park, S. H., Kim, I. B., Kang, W. S., Oh, S. J., & Chun, M. H. (2002). Light- and electron-microscopic analysis of vasoactive intestinal polypeptide-immunoreactive amacrine cells in the guinea pig retina. The Journal of Comparative Neurology, 445(4), pp. 325-35.
Lee EJ, et al. Light- and Electron-microscopic Analysis of Vasoactive Intestinal Polypeptide-immunoreactive Amacrine Cells in the Guinea Pig Retina. J Comp Neurol. 2002 Apr 15;445(4):325-35. PubMed PMID: 11920710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light- and electron-microscopic analysis of vasoactive intestinal polypeptide-immunoreactive amacrine cells in the guinea pig retina. AU - Lee,Eun-Jin, AU - Park,Sung-Hee, AU - Kim,In-Beom, AU - Kang,Wha-Sun, AU - Oh,Su-Ja, AU - Chun,Myung-Hoon, PY - 2002/3/29/pubmed PY - 2002/5/11/medline PY - 2002/3/29/entrez SP - 325 EP - 35 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 445 IS - 4 N2 - Vasoactive intestinal polypeptide (VIP) is a neuroactive substance that is expressed in both nonmammalian and mammalian retinas. This study investigated the morphology and synaptic connections of VIP-containing neurons in the guinea pig retina by immunocytochemistry, by using antisera against VIP. Specific VIP immunoreactivity was localized to a population of wide-field and regularly spaced amacrine cells with processes ramifying mainly in strata 1 and 2 of the inner plexiform layer (IPL). Double-label immunohistochemistry demonstrated that all VIP-immunoreactive cells possessed gamma-aminobutyric acid immunoreactivity. The synaptic connectivity of VIP-immunoreactive amacrine cells was identified in the IPL by electron microscopy. The VIP-labeled amacrine cell processes received synaptic input from other amacrine cell processes and bipolar cell axon terminals in strata 1 to 3 of the IPL. The most frequent postsynaptic targets of VIP-immunoreactive amacrine cells were other amacrine cell processes in strata 1 to 3 of the IPL. Synaptic outputs to bipolar cells were also observed in strata 1 to 3 of the IPL. In addition, ganglion cell dendrites were also postsynaptic to VIP-immunoreactive neurons in the sublamina a of the IPL. These studies show that one type of VIP-immunoreactive amacrine cells make contact predominantly with other amacrine cell processes. This finding suggests that VIP-containing amacrine cells may influence inner retinal circuitry, thus mediating visual processing. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/11920710/Light__and_electron_microscopic_analysis_of_vasoactive_intestinal_polypeptide_immunoreactive_amacrine_cells_in_the_guinea_pig_retina_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=2002&volume=445&issue=4&spage=325 DB - PRIME DP - Unbound Medicine ER -