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Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans.
J Clin Microbiol. 2002 Apr; 40(4):1441-6.JC

Abstract

By using sequence analysis of Shiga toxin 1 (Stx 1) genes from human and ovine Stx-producing Escherichia coli (STEC) strains, we identified an Stx1 variant in STEC of human origin that was identical to the Stx1 variant from ovine STEC, but demonstrated only 97.1 and 96.6% amino acid sequence identity in its A and B subunits, respectively, to the Stx1 encoded by bacteriophage 933J. We designated this variant "Stx1c" and developed stxB(1) restriction fragment length polymorphism and stx(1c)-specific PCR strategies to determine the frequency and distribution of stx(1c) among 212 STEC strains isolated from humans. stx(1c) was identified in 36 (17.0%) of 212 STEC strains, 19 of which originated from asymptomatic subjects and 16 of which were from patients with uncomplicated diarrhea. stx(1c) was most frequently (in 23 STEC strains [63.9%]) associated with stx(2d), but 12 (33.3%) of the 36 STEC strains possessed stx(1c) only. A single STEC strain possessed stx(1c) together with stx(2) and was isolated from a patient with hemolytic-uremic syndrome. All 36 stx(1c)-positive STEC strains were eae negative and belonged to 10 different serogroups, none of which was O157, O26, O103, O111, or O145. Stx1c was produced by all stx(1c)-containing STEC strains, but reacted weakly with a commercial immunoassay. We conclude that STEC strains harboring the stx(1c) variant account for a significant proportion of human STEC isolates. The procedures developed in this study now allow the determination of the frequency of STEC strains harboring stx(1c) among clinical STEC isolates and their association with human disease in prospective studies.

Authors+Show Affiliations

Institut für Hygiene, Universitätsklinikum Münster, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11923370

Citation

Zhang, Wenlan, et al. "Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx(1c)) in Escherichia Coli Strains Isolated From Humans." Journal of Clinical Microbiology, vol. 40, no. 4, 2002, pp. 1441-6.
Zhang W, Bielaszewska M, Kuczius T, et al. Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans. J Clin Microbiol. 2002;40(4):1441-6.
Zhang, W., Bielaszewska, M., Kuczius, T., & Karch, H. (2002). Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans. Journal of Clinical Microbiology, 40(4), 1441-6.
Zhang W, et al. Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx(1c)) in Escherichia Coli Strains Isolated From Humans. J Clin Microbiol. 2002;40(4):1441-6. PubMed PMID: 11923370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans. AU - Zhang,Wenlan, AU - Bielaszewska,Martina, AU - Kuczius,Thorsten, AU - Karch,Helge, PY - 2002/3/30/pubmed PY - 2002/7/18/medline PY - 2002/3/30/entrez SP - 1441 EP - 6 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 40 IS - 4 N2 - By using sequence analysis of Shiga toxin 1 (Stx 1) genes from human and ovine Stx-producing Escherichia coli (STEC) strains, we identified an Stx1 variant in STEC of human origin that was identical to the Stx1 variant from ovine STEC, but demonstrated only 97.1 and 96.6% amino acid sequence identity in its A and B subunits, respectively, to the Stx1 encoded by bacteriophage 933J. We designated this variant "Stx1c" and developed stxB(1) restriction fragment length polymorphism and stx(1c)-specific PCR strategies to determine the frequency and distribution of stx(1c) among 212 STEC strains isolated from humans. stx(1c) was identified in 36 (17.0%) of 212 STEC strains, 19 of which originated from asymptomatic subjects and 16 of which were from patients with uncomplicated diarrhea. stx(1c) was most frequently (in 23 STEC strains [63.9%]) associated with stx(2d), but 12 (33.3%) of the 36 STEC strains possessed stx(1c) only. A single STEC strain possessed stx(1c) together with stx(2) and was isolated from a patient with hemolytic-uremic syndrome. All 36 stx(1c)-positive STEC strains were eae negative and belonged to 10 different serogroups, none of which was O157, O26, O103, O111, or O145. Stx1c was produced by all stx(1c)-containing STEC strains, but reacted weakly with a commercial immunoassay. We conclude that STEC strains harboring the stx(1c) variant account for a significant proportion of human STEC isolates. The procedures developed in this study now allow the determination of the frequency of STEC strains harboring stx(1c) among clinical STEC isolates and their association with human disease in prospective studies. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11923370/Identification_characterization_and_distribution_of_a_Shiga_toxin_1_gene_variant__stx_1c___in_Escherichia_coli_strains_isolated_from_humans_ L2 - https://journals.asm.org/doi/10.1128/JCM.40.4.1441-1446.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -