Tags

Type your tag names separated by a space and hit enter

Microdialysis of dopamine in the nucleus accumbens of alcohol-preferring (P) rats during anticipation and operant self-administration of ethanol.
Alcohol Clin Exp Res. 2002 Mar; 26(3):318-25.AC

Abstract

BACKGROUND

The present study was designed to test directly whether a contextual stimulus for access to ethanol would acquire the ability to enhance locomotor activity and dopamine efflux in the nucleus accumbens (NAc) of alcohol-preferring (P) rats. The study also explored the association between elevated locomotor activity and NAc dopamine efflux during operant self-administration of ethanol.

METHODS

Adult female P rats were randomly assigned to operantly self-administer either 15% (v/v) ethanol or 0.0125% (w/v) saccharin. Both groups were trained in a daily 30-min two-lever concurrent operant task (FR-3) to orally self-administer ethanol or saccharin, with water on the alternate lever. A third (control) group was trained to self-administer water on both levers. All groups were also acclimated in the operant chambers to periods of habituation, anticipation, and postadministration.

RESULTS

Compared with controls, the ethanol group, but not the saccharin group, showed significant increases in locomotor activity as well as increased NAc dopamine efflux during the first 10 min of the anticipation period. During the first 10 min of the self-administration period, locomotor activity was significantly increased in both the ethanol and saccharin groups compared with control values. The ethanol group, but not the saccharin group, showed significant increases in NAc dopamine efflux during the 20th and 30th min of the self-administration period and during the first 10 min of the postadministration period.

CONCLUSIONS

The findings suggest that acquisition of signal-induced anticipation of self-administered ethanol is associated with increases in locomotor activity and extracellular levels of dopamine in the NAc of P rats. Such associations may be important to the development and maintenance of ethanol-seeking behaviors. The findings also indicate that operant self-administration of ethanol is associated with increases in extracellular levels of dopamine in the NAc of P rats.

Authors+Show Affiliations

Department of Psychology, Program in Psychobiology of Addictions, Purdue School of Science/IUPUI, 402 N. Blackford Street, Indianapolis, Indiana 46202-3275, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11923583

Citation

Melendez, Roberto I., et al. "Microdialysis of Dopamine in the Nucleus Accumbens of Alcohol-preferring (P) Rats During Anticipation and Operant Self-administration of Ethanol." Alcoholism, Clinical and Experimental Research, vol. 26, no. 3, 2002, pp. 318-25.
Melendez RI, Rodd-Henricks ZA, Engleman EA, et al. Microdialysis of dopamine in the nucleus accumbens of alcohol-preferring (P) rats during anticipation and operant self-administration of ethanol. Alcohol Clin Exp Res. 2002;26(3):318-25.
Melendez, R. I., Rodd-Henricks, Z. A., Engleman, E. A., Li, T. K., McBride, W. J., & Murphy, J. M. (2002). Microdialysis of dopamine in the nucleus accumbens of alcohol-preferring (P) rats during anticipation and operant self-administration of ethanol. Alcoholism, Clinical and Experimental Research, 26(3), 318-25.
Melendez RI, et al. Microdialysis of Dopamine in the Nucleus Accumbens of Alcohol-preferring (P) Rats During Anticipation and Operant Self-administration of Ethanol. Alcohol Clin Exp Res. 2002;26(3):318-25. PubMed PMID: 11923583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microdialysis of dopamine in the nucleus accumbens of alcohol-preferring (P) rats during anticipation and operant self-administration of ethanol. AU - Melendez,Roberto I, AU - Rodd-Henricks,Zachary A, AU - Engleman,Eric A, AU - Li,Ting-Kai, AU - McBride,William J, AU - Murphy,James M, PY - 2002/3/30/pubmed PY - 2002/7/26/medline PY - 2002/3/30/entrez SP - 318 EP - 25 JF - Alcoholism, clinical and experimental research JO - Alcohol Clin Exp Res VL - 26 IS - 3 N2 - BACKGROUND: The present study was designed to test directly whether a contextual stimulus for access to ethanol would acquire the ability to enhance locomotor activity and dopamine efflux in the nucleus accumbens (NAc) of alcohol-preferring (P) rats. The study also explored the association between elevated locomotor activity and NAc dopamine efflux during operant self-administration of ethanol. METHODS: Adult female P rats were randomly assigned to operantly self-administer either 15% (v/v) ethanol or 0.0125% (w/v) saccharin. Both groups were trained in a daily 30-min two-lever concurrent operant task (FR-3) to orally self-administer ethanol or saccharin, with water on the alternate lever. A third (control) group was trained to self-administer water on both levers. All groups were also acclimated in the operant chambers to periods of habituation, anticipation, and postadministration. RESULTS: Compared with controls, the ethanol group, but not the saccharin group, showed significant increases in locomotor activity as well as increased NAc dopamine efflux during the first 10 min of the anticipation period. During the first 10 min of the self-administration period, locomotor activity was significantly increased in both the ethanol and saccharin groups compared with control values. The ethanol group, but not the saccharin group, showed significant increases in NAc dopamine efflux during the 20th and 30th min of the self-administration period and during the first 10 min of the postadministration period. CONCLUSIONS: The findings suggest that acquisition of signal-induced anticipation of self-administered ethanol is associated with increases in locomotor activity and extracellular levels of dopamine in the NAc of P rats. Such associations may be important to the development and maintenance of ethanol-seeking behaviors. The findings also indicate that operant self-administration of ethanol is associated with increases in extracellular levels of dopamine in the NAc of P rats. SN - 0145-6008 UR - https://www.unboundmedicine.com/medline/citation/11923583/Microdialysis_of_dopamine_in_the_nucleus_accumbens_of_alcohol_preferring__P__rats_during_anticipation_and_operant_self_administration_of_ethanol_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11923583.ui DB - PRIME DP - Unbound Medicine ER -