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Isolation, purification, and structure of components from acidic polysaccharides of Pleurotus ostreatus (Fr.) Quél.
Carbohydr Res. 1975 Sep; 43(2):305-20.CR

Abstract

Isolation of an antitumor component from polysaccharide fraction A5 of some Basidiomyces was achieved by column chromatography on Sephadex G-200. A detection method based on the specific rotatory characteristics of the polysaccharide was applied to estimate components in effluent fractions from the chromatography, and it was confirmed that a series of eluates having similar specific rotation was made up of homogeneous polysaccharide. Three components (H51, H52, and H53) were isolated, in chromatographically pure state, from fraction A5. Component H51 consisted of a skeleton of beta-(1 leads to 3)-linked glucose residues, probably having branches of galactose and mannose residues, and also containing acidic sugars. Component H53 had a main structure similarly consisting of beta-(1 leads to 3)-linked glucose residues and a larger proportion of acidic sugar than H51. Component H52 was a heteropolysaccharide made up of alpha-linked galactose and mannose residues. Components H51 and H53 had a higher and a lower molecular weight, respectively, than H52. The only antitumor-active component was H51.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

1192437

Citation

Yoshioka, Y, et al. "Isolation, Purification, and Structure of Components From Acidic Polysaccharides of Pleurotus Ostreatus (Fr.) Quél." Carbohydrate Research, vol. 43, no. 2, 1975, pp. 305-20.
Yoshioka Y, Emori M, Ikekawa T, et al. Isolation, purification, and structure of components from acidic polysaccharides of Pleurotus ostreatus (Fr.) Quél. Carbohydr Res. 1975;43(2):305-20.
Yoshioka, Y., Emori, M., Ikekawa, T., & Fukuoka, F. (1975). Isolation, purification, and structure of components from acidic polysaccharides of Pleurotus ostreatus (Fr.) Quél. Carbohydrate Research, 43(2), 305-20.
Yoshioka Y, et al. Isolation, Purification, and Structure of Components From Acidic Polysaccharides of Pleurotus Ostreatus (Fr.) Quél. Carbohydr Res. 1975;43(2):305-20. PubMed PMID: 1192437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation, purification, and structure of components from acidic polysaccharides of Pleurotus ostreatus (Fr.) Quél. AU - Yoshioka,Y, AU - Emori,M, AU - Ikekawa,T, AU - Fukuoka,F, PY - 1975/9/1/pubmed PY - 1975/9/1/medline PY - 1975/9/1/entrez SP - 305 EP - 20 JF - Carbohydrate research JO - Carbohydr Res VL - 43 IS - 2 N2 - Isolation of an antitumor component from polysaccharide fraction A5 of some Basidiomyces was achieved by column chromatography on Sephadex G-200. A detection method based on the specific rotatory characteristics of the polysaccharide was applied to estimate components in effluent fractions from the chromatography, and it was confirmed that a series of eluates having similar specific rotation was made up of homogeneous polysaccharide. Three components (H51, H52, and H53) were isolated, in chromatographically pure state, from fraction A5. Component H51 consisted of a skeleton of beta-(1 leads to 3)-linked glucose residues, probably having branches of galactose and mannose residues, and also containing acidic sugars. Component H53 had a main structure similarly consisting of beta-(1 leads to 3)-linked glucose residues and a larger proportion of acidic sugar than H51. Component H52 was a heteropolysaccharide made up of alpha-linked galactose and mannose residues. Components H51 and H53 had a higher and a lower molecular weight, respectively, than H52. The only antitumor-active component was H51. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/1192437/Isolation_purification_and_structure_of_components_from_acidic_polysaccharides_of_Pleurotus_ostreatus__Fr___Quél_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(00)83495-3 DB - PRIME DP - Unbound Medicine ER -