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Induction of fibronectin mRNA by urokinase- and tissue-type plasminogen activator in human skin fibroblasts: differential role of u-PA and t-PA at the fibronectin protein level.
Biol Chem. 2002 Jan; 383(1):177-87.BC

Abstract

Plasminogen activators of the urokinase- and tissue-type and fetal calf serum (u-PA, t-PA, FCS) exert their mitogenic effect on quiescent human dermal fibroblasts and modulate the mRNA expression of cell-cycle related genes. The present study deals with the effects of PAs on the expression of fibronectin (FN), a heterodimeric extracellular matrix (ECM) protein that can be modulated in different ways by various mitogens. The kinetics of FN gene response was examined in quiescent fibroblasts upon PA stimulation (30 min -24 h). The results obtained evidenced that: (i) all mitogens tested (u-PA, t-PA and FCS) led to an increase of FN mRNA expression in early G1, as shown by the analysis of two sequences, III-9, common to all FN mRNAs, and EDA+, present only in the EDA+FN isoform; (ii) the kinetic profiles of FN mRNA stimulation were comparable for the three mitogens, although the effects on the FN-ECM assembly were distinct; (iii) t-PA and FCS led to FN assembly in the ECM, which was absent or decreased in u-PA-treated cultures. Immunobiochemical analysis of total FN and EDA+ FN showed that FN induced by t-PA was mainly dimeric (450-500 kDa), whereas FN induced by u-PA was mainly monomeric (230-250 kDa). These differences are probably due to the differential enzymatic action of t-PA and u-PA on FN, which might be related to a differential role of the two PAs in several physiopathological conditions.

Authors+Show Affiliations

Department of Biomedical Sciences and Biotechnologies, University of Brescia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11928812

Citation

De Petro, Giuseppina, et al. "Induction of Fibronectin mRNA By Urokinase- and Tissue-type Plasminogen Activator in Human Skin Fibroblasts: Differential Role of u-PA and t-PA at the Fibronectin Protein Level." Biological Chemistry, vol. 383, no. 1, 2002, pp. 177-87.
De Petro G, Tavian D, Marchina E, et al. Induction of fibronectin mRNA by urokinase- and tissue-type plasminogen activator in human skin fibroblasts: differential role of u-PA and t-PA at the fibronectin protein level. Biol Chem. 2002;383(1):177-87.
De Petro, G., Tavian, D., Marchina, E., & Barlati, S. (2002). Induction of fibronectin mRNA by urokinase- and tissue-type plasminogen activator in human skin fibroblasts: differential role of u-PA and t-PA at the fibronectin protein level. Biological Chemistry, 383(1), 177-87.
De Petro G, et al. Induction of Fibronectin mRNA By Urokinase- and Tissue-type Plasminogen Activator in Human Skin Fibroblasts: Differential Role of u-PA and t-PA at the Fibronectin Protein Level. Biol Chem. 2002;383(1):177-87. PubMed PMID: 11928812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of fibronectin mRNA by urokinase- and tissue-type plasminogen activator in human skin fibroblasts: differential role of u-PA and t-PA at the fibronectin protein level. AU - De Petro,Giuseppina, AU - Tavian,Daniela, AU - Marchina,Eleonora, AU - Barlati,Sergio, PY - 2002/4/4/pubmed PY - 2003/2/14/medline PY - 2002/4/4/entrez SP - 177 EP - 87 JF - Biological chemistry JO - Biol Chem VL - 383 IS - 1 N2 - Plasminogen activators of the urokinase- and tissue-type and fetal calf serum (u-PA, t-PA, FCS) exert their mitogenic effect on quiescent human dermal fibroblasts and modulate the mRNA expression of cell-cycle related genes. The present study deals with the effects of PAs on the expression of fibronectin (FN), a heterodimeric extracellular matrix (ECM) protein that can be modulated in different ways by various mitogens. The kinetics of FN gene response was examined in quiescent fibroblasts upon PA stimulation (30 min -24 h). The results obtained evidenced that: (i) all mitogens tested (u-PA, t-PA and FCS) led to an increase of FN mRNA expression in early G1, as shown by the analysis of two sequences, III-9, common to all FN mRNAs, and EDA+, present only in the EDA+FN isoform; (ii) the kinetic profiles of FN mRNA stimulation were comparable for the three mitogens, although the effects on the FN-ECM assembly were distinct; (iii) t-PA and FCS led to FN assembly in the ECM, which was absent or decreased in u-PA-treated cultures. Immunobiochemical analysis of total FN and EDA+ FN showed that FN induced by t-PA was mainly dimeric (450-500 kDa), whereas FN induced by u-PA was mainly monomeric (230-250 kDa). These differences are probably due to the differential enzymatic action of t-PA and u-PA on FN, which might be related to a differential role of the two PAs in several physiopathological conditions. SN - 1431-6730 UR - https://www.unboundmedicine.com/medline/citation/11928812/Induction_of_fibronectin_mRNA_by_urokinase__and_tissue_type_plasminogen_activator_in_human_skin_fibroblasts:_differential_role_of_u_PA_and_t_PA_at_the_fibronectin_protein_level_ L2 - https://www.degruyter.com/document/doi/10.1515/BC.2002.018 DB - PRIME DP - Unbound Medicine ER -