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Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce.

Abstract

Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C(18) column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C(30) column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 microg/dL (from 0.664 to 1.30 microM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was approximately 12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum.

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  • Authors+Show Affiliations

    ,

    Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, USA. breemen@uic.edu

    , , , , , ,

    Source

    MeSH

    Adenocarcinoma
    Aged
    Biopsy, Needle
    Carotenoids
    Chromatography, High Pressure Liquid
    Chromatography, Liquid
    Diet
    Humans
    Isomerism
    Lycopene
    Lycopersicon esculentum
    Male
    Mass Spectrometry
    Middle Aged
    Prostate
    Prostatectomy
    Prostatic Neoplasms

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    11929273

    Citation

    van Breemen, Richard B., et al. "Liquid Chromatography-mass Spectrometry of Cis- and All-trans-lycopene in Human Serum and Prostate Tissue After Dietary Supplementation With Tomato Sauce." Journal of Agricultural and Food Chemistry, vol. 50, no. 8, 2002, pp. 2214-9.
    van Breemen RB, Xu X, Viana MA, et al. Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce. J Agric Food Chem. 2002;50(8):2214-9.
    van Breemen, R. B., Xu, X., Viana, M. A., Chen, L., Stacewicz-Sapuntzakis, M., Duncan, C., ... Sharifi, R. (2002). Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce. Journal of Agricultural and Food Chemistry, 50(8), pp. 2214-9.
    van Breemen RB, et al. Liquid Chromatography-mass Spectrometry of Cis- and All-trans-lycopene in Human Serum and Prostate Tissue After Dietary Supplementation With Tomato Sauce. J Agric Food Chem. 2002 Apr 10;50(8):2214-9. PubMed PMID: 11929273.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce. AU - van Breemen,Richard B, AU - Xu,Xiaoying, AU - Viana,Marlos A, AU - Chen,Longwen, AU - Stacewicz-Sapuntzakis,Maria, AU - Duncan,Claudine, AU - Bowen,Phyllis E, AU - Sharifi,Roohollah, PY - 2002/4/4/pubmed PY - 2002/5/30/medline PY - 2002/4/4/entrez SP - 2214 EP - 9 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 50 IS - 8 N2 - Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C(18) column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C(30) column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 microg/dL (from 0.664 to 1.30 microM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was approximately 12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/11929273/Liquid_chromatography_mass_spectrometry_of_cis__and_all_trans_lycopene_in_human_serum_and_prostate_tissue_after_dietary_supplementation_with_tomato_sauce_ L2 - https://dx.doi.org/10.1021/jf0110351 DB - PRIME DP - Unbound Medicine ER -