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The alternative sigma factor sigmaE controls antioxidant defences required for Salmonella virulence and stationary-phase survival.
Mol Microbiol. 2002 Feb; 43(3):771-82.MM

Abstract

Bacteria must contend with conditions of nutrient limitation in all natural environments. Complex programmes of gene expression, controlled in part by the alternative sigma factors sigmaS (sigma38, RpoS) and sigmaH (sigma32, RpoH), allow a number of bacterial species to survive conditions of partial or complete starvation. We show here that the alternative sigma factor sigmaE (sigma24, RpoE) also facilitates the survival of Salmonella typhimurium under conditions of nutrient deprivation. Expression of the sigmaE regulon is strongly induced upon entry of Salmonella into stationary phase. A Salmonella mutant lacking sigmaE has reduced survival during stationary phase as well as increased susceptibility to oxidative stress. A Salmonella strain lacking both sigmaE and sigmaS is non-viable after just 24 h in stationary phase, but survival of these mutants is completely preserved under anaerobic stationary-phase conditions, suggesting that oxidative injury is one of the major mechanisms of reduced microbial viability during periods of nutrient deprivation. Moreover, the attenuated virulence of sigmaE-deficient Salmonella for mice can be largely restored by genetic abrogation of the host phagocyte respiratory burst, suggesting that the sigmaE regulon plays an important antioxidant role during Salmonella infection of mammalian hosts.

Authors+Show Affiliations

Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11929531

Citation

Testerman, Traci L., et al. "The Alternative Sigma Factor sigmaE Controls Antioxidant Defences Required for Salmonella Virulence and Stationary-phase Survival." Molecular Microbiology, vol. 43, no. 3, 2002, pp. 771-82.
Testerman TL, Vazquez-Torres A, Xu Y, et al. The alternative sigma factor sigmaE controls antioxidant defences required for Salmonella virulence and stationary-phase survival. Mol Microbiol. 2002;43(3):771-82.
Testerman, T. L., Vazquez-Torres, A., Xu, Y., Jones-Carson, J., Libby, S. J., & Fang, F. C. (2002). The alternative sigma factor sigmaE controls antioxidant defences required for Salmonella virulence and stationary-phase survival. Molecular Microbiology, 43(3), 771-82.
Testerman TL, et al. The Alternative Sigma Factor sigmaE Controls Antioxidant Defences Required for Salmonella Virulence and Stationary-phase Survival. Mol Microbiol. 2002;43(3):771-82. PubMed PMID: 11929531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The alternative sigma factor sigmaE controls antioxidant defences required for Salmonella virulence and stationary-phase survival. AU - Testerman,Traci L, AU - Vazquez-Torres,Andrés, AU - Xu,Yisheng, AU - Jones-Carson,Jessica, AU - Libby,Stephen J, AU - Fang,Ferric C, PY - 2002/4/4/pubmed PY - 2002/6/5/medline PY - 2002/4/4/entrez SP - 771 EP - 82 JF - Molecular microbiology JO - Mol Microbiol VL - 43 IS - 3 N2 - Bacteria must contend with conditions of nutrient limitation in all natural environments. Complex programmes of gene expression, controlled in part by the alternative sigma factors sigmaS (sigma38, RpoS) and sigmaH (sigma32, RpoH), allow a number of bacterial species to survive conditions of partial or complete starvation. We show here that the alternative sigma factor sigmaE (sigma24, RpoE) also facilitates the survival of Salmonella typhimurium under conditions of nutrient deprivation. Expression of the sigmaE regulon is strongly induced upon entry of Salmonella into stationary phase. A Salmonella mutant lacking sigmaE has reduced survival during stationary phase as well as increased susceptibility to oxidative stress. A Salmonella strain lacking both sigmaE and sigmaS is non-viable after just 24 h in stationary phase, but survival of these mutants is completely preserved under anaerobic stationary-phase conditions, suggesting that oxidative injury is one of the major mechanisms of reduced microbial viability during periods of nutrient deprivation. Moreover, the attenuated virulence of sigmaE-deficient Salmonella for mice can be largely restored by genetic abrogation of the host phagocyte respiratory burst, suggesting that the sigmaE regulon plays an important antioxidant role during Salmonella infection of mammalian hosts. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/11929531/The_alternative_sigma_factor_sigmaE_controls_antioxidant_defences_required_for_Salmonella_virulence_and_stationary_phase_survival_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2002&volume=43&issue=3&spage=771 DB - PRIME DP - Unbound Medicine ER -