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Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants.
Appl Microbiol Biotechnol. 2002 Mar; 58(3):370-7.AM

Abstract

The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-beta-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkalysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of beta-(1,6)-D-linkage than that from the wild-type.

Authors+Show Affiliations

Ha CH
Graduate School of Biotechnology, Korea University, Seoul, South Korea.
Lim KH
No affiliation info available
Kim YT
No affiliation info available
Lim ST
No affiliation info available
Kim CW
No affiliation info available
Chang HI
No affiliation info available

MeSH

AlkaliesCell WallChromatography, GasChromatography, High Pressure LiquidGlucan Endo-1,3-beta-D-GlucosidaseGlucansMagnetic Resonance SpectroscopyMolecular StructureMutationPolysaccharides, BacterialSaccharomyces cerevisiaeSolubility

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11935190

Citation

Ha, C H., et al. "Analysis of Alkali-soluble Glucan Produced By Saccharomyces Cerevisiae Wild-type and Mutants." Applied Microbiology and Biotechnology, vol. 58, no. 3, 2002, pp. 370-7.
Ha CH, Lim KH, Kim YT, et al. Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants. Appl Microbiol Biotechnol. 2002;58(3):370-7.
Ha, C. H., Lim, K. H., Kim, Y. T., Lim, S. T., Kim, C. W., & Chang, H. I. (2002). Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants. Applied Microbiology and Biotechnology, 58(3), 370-7.
Ha CH, et al. Analysis of Alkali-soluble Glucan Produced By Saccharomyces Cerevisiae Wild-type and Mutants. Appl Microbiol Biotechnol. 2002;58(3):370-7. PubMed PMID: 11935190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants. AU - Ha,C H, AU - Lim,K H, AU - Kim,Y T, AU - Lim,S T, AU - Kim,C W, AU - Chang,H I, Y1 - 2002/01/10/ PY - 2001/04/27/received PY - 2001/08/24/accepted PY - 2002/4/6/pubmed PY - 2002/11/26/medline PY - 2002/4/6/entrez SP - 370 EP - 7 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 58 IS - 3 N2 - The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-beta-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkalysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of beta-(1,6)-D-linkage than that from the wild-type. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/11935190/Analysis_of_alkali_soluble_glucan_produced_by_Saccharomyces_cerevisiae_wild_type_and_mutants_ L2 - https://dx.doi.org/10.1007/s002530100824 DB - PRIME DP - Unbound Medicine ER -