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Sensitive and simple method for the determination of nicotine and cotinine in human urine, plasma and saliva by gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Mar 25; 769(1):177-83.JC

Abstract

A method is proposed for the determination of nicotine and cotinine in human urine, plasma and saliva. Nicotine and cotinine were extracted from alkalinized sample with ethyl ether and concentrated to minimum volume with nitrogen stream. The volatility of nicotine was prevented by the addition of acetic acid to the organic solvent during evaporation. Peak shapes and quantitation of nicotine and cotinine are excellent, with linear calibration curves over a wide range of 1-10,000 ng/ml. The detection limits of nicotine and cotinine are 0.2 ng/ml in urine and 1.0 ng/ml in plasma and saliva. The intra-day precision of nicotine and cotinine in all samples was <5% relative standard deviation (RSD). Urine, plasma and saliva samples of 303 non-smoking and 41 smoking volunteers from a girl's high school in Korea were quantified by the described procedure. As a result, the concentrations of nicotine and cotinine in plasma ranged from 6 to 498 ng/ml and 4 to 96 ng/ml. Otherwise, those of nicotine and cotinine in saliva ranged from 0 to 207 ng/ml and 0 to 42 ng/ml, and those of nicotine and cotinine in urine ranged from 0 to 1,590 ng/ml and 0 to 2,986 ng/ml, respectively. We found that the concentration of cotinine in plasma was successfully predicted from the salivary cotinine concentration by the equation y=2.31x+4.76 (x=the concentration of cotinine in saliva, y=the concentration of cotinine in plasma). The results show that through the accurate determination of cotinine in saliva, the risk of ETS-exposed human can be predicted.

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Authors+Show Affiliations

Shin HS
Department of Environmental Education, Kongju National University, Kongju, South Korea. hshin@kongju.ac.kr
Kim JG
No affiliation info available
Shin YJ
No affiliation info available
Jee SH
No affiliation info available

MeSH

CotinineGas Chromatography-Mass SpectrometryHumansNicotineReproducibility of ResultsSalivaSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11936690

Citation

Shin, Ho-Sang, et al. "Sensitive and Simple Method for the Determination of Nicotine and Cotinine in Human Urine, Plasma and Saliva By Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 769, no. 1, 2002, pp. 177-83.
Shin HS, Kim JG, Shin YJ, et al. Sensitive and simple method for the determination of nicotine and cotinine in human urine, plasma and saliva by gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;769(1):177-83.
Shin, H. S., Kim, J. G., Shin, Y. J., & Jee, S. H. (2002). Sensitive and simple method for the determination of nicotine and cotinine in human urine, plasma and saliva by gas chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 769(1), 177-83.
Shin HS, et al. Sensitive and Simple Method for the Determination of Nicotine and Cotinine in Human Urine, Plasma and Saliva By Gas Chromatography-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Mar 25;769(1):177-83. PubMed PMID: 11936690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive and simple method for the determination of nicotine and cotinine in human urine, plasma and saliva by gas chromatography-mass spectrometry. AU - Shin,Ho-Sang, AU - Kim,Jin-Gu, AU - Shin,Yoon-Jeong, AU - Jee,Sun Ha, PY - 2002/4/9/pubmed PY - 2002/10/3/medline PY - 2002/4/9/entrez SP - 177 EP - 83 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 769 IS - 1 N2 - A method is proposed for the determination of nicotine and cotinine in human urine, plasma and saliva. Nicotine and cotinine were extracted from alkalinized sample with ethyl ether and concentrated to minimum volume with nitrogen stream. The volatility of nicotine was prevented by the addition of acetic acid to the organic solvent during evaporation. Peak shapes and quantitation of nicotine and cotinine are excellent, with linear calibration curves over a wide range of 1-10,000 ng/ml. The detection limits of nicotine and cotinine are 0.2 ng/ml in urine and 1.0 ng/ml in plasma and saliva. The intra-day precision of nicotine and cotinine in all samples was <5% relative standard deviation (RSD). Urine, plasma and saliva samples of 303 non-smoking and 41 smoking volunteers from a girl's high school in Korea were quantified by the described procedure. As a result, the concentrations of nicotine and cotinine in plasma ranged from 6 to 498 ng/ml and 4 to 96 ng/ml. Otherwise, those of nicotine and cotinine in saliva ranged from 0 to 207 ng/ml and 0 to 42 ng/ml, and those of nicotine and cotinine in urine ranged from 0 to 1,590 ng/ml and 0 to 2,986 ng/ml, respectively. We found that the concentration of cotinine in plasma was successfully predicted from the salivary cotinine concentration by the equation y=2.31x+4.76 (x=the concentration of cotinine in saliva, y=the concentration of cotinine in plasma). The results show that through the accurate determination of cotinine in saliva, the risk of ETS-exposed human can be predicted. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/11936690/Sensitive_and_simple_method_for_the_determination_of_nicotine_and_cotinine_in_human_urine_plasma_and_saliva_by_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(02)00007-7 DB - PRIME DP - Unbound Medicine ER -