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On-column enzyme-catalyzed microreactions using capillary electrophoresis: quantitative studies.
J Capill Electrophor Microchip Technol. 2002; 7(1-2):1-9.JC

Abstract

Capillary electrophoresis and on-column enzyme-catalyzed microreactor techniques were used to quantitate the reaction projects resulting from three model systems: i) the conversion of nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide, reduced form (NADH) in the oxidation of glucose-6-phosphate (glc-6-p) to 6-phosphogluconate by glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49); ii) the conversion of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and adenosine monophosphate (AMP) by hexokinase (HK, EC 2.7.1.1) and apyrase (APY, EC 3.6.1.5), respectively, in the conversion of glucose to glucose-6-phosphate and inorganic phosphate, respectively, and; iii) the conversion of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate by fructose-biphosphate aldolase (ALD, EC 4.1.2.13). Single and double microreactor techniques employing direct or indirect detection were used to follow the conversion of substrate to product(s). In addition, electrophoresis conditions including voltage, enzyme concentration, and mixing time of the reaction, were correlated to product distribution profiles.

Authors+Show Affiliations

Zhang Y
Department of Chemistry and Biochemistry, California State University, Los Angeles 90032-8202, USA. fgomez2@calstatela.edu
Kaddis J
No affiliation info available
Silverio C
No affiliation info available
Zurita C
No affiliation info available
Gomez FA
No affiliation info available

MeSH

Adenosine DiphosphateAdenosine TriphosphateApyraseBioreactorsElectrophoresis, CapillaryEnzymesFructose-Bisphosphate AldolaseGlucosephosphate DehydrogenaseHexokinaseMicrochemistryNADOxidation-ReductionReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11936761

Citation

Zhang, Ying, et al. "On-column Enzyme-catalyzed Microreactions Using Capillary Electrophoresis: Quantitative Studies." Journal of Capillary Electrophoresis and Microchip Technology, vol. 7, no. 1-2, 2002, pp. 1-9.
Zhang Y, Kaddis J, Silverio C, et al. On-column enzyme-catalyzed microreactions using capillary electrophoresis: quantitative studies. J Capill Electrophor Microchip Technol. 2002;7(1-2):1-9.
Zhang, Y., Kaddis, J., Silverio, C., Zurita, C., & Gomez, F. A. (2002). On-column enzyme-catalyzed microreactions using capillary electrophoresis: quantitative studies. Journal of Capillary Electrophoresis and Microchip Technology, 7(1-2), 1-9.
Zhang Y, et al. On-column Enzyme-catalyzed Microreactions Using Capillary Electrophoresis: Quantitative Studies. J Capill Electrophor Microchip Technol. 2002;7(1-2):1-9. PubMed PMID: 11936761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-column enzyme-catalyzed microreactions using capillary electrophoresis: quantitative studies. AU - Zhang,Ying, AU - Kaddis,John, AU - Silverio,Catherine, AU - Zurita,Cecilia, AU - Gomez,Frank A, PY - 2002/4/9/pubmed PY - 2002/5/15/medline PY - 2002/4/9/entrez SP - 1 EP - 9 JF - Journal of capillary electrophoresis and microchip technology JO - J Capill Electrophor Microchip Technol VL - 7 IS - 1-2 N2 - Capillary electrophoresis and on-column enzyme-catalyzed microreactor techniques were used to quantitate the reaction projects resulting from three model systems: i) the conversion of nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide, reduced form (NADH) in the oxidation of glucose-6-phosphate (glc-6-p) to 6-phosphogluconate by glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49); ii) the conversion of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and adenosine monophosphate (AMP) by hexokinase (HK, EC 2.7.1.1) and apyrase (APY, EC 3.6.1.5), respectively, in the conversion of glucose to glucose-6-phosphate and inorganic phosphate, respectively, and; iii) the conversion of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate by fructose-biphosphate aldolase (ALD, EC 4.1.2.13). Single and double microreactor techniques employing direct or indirect detection were used to follow the conversion of substrate to product(s). In addition, electrophoresis conditions including voltage, enzyme concentration, and mixing time of the reaction, were correlated to product distribution profiles. SN - 1946-4940 UR - https://www.unboundmedicine.com/medline/citation/11936761/On_column_enzyme_catalyzed_microreactions_using_capillary_electrophoresis:_quantitative_studies_ L2 - https://antibodies.cancer.gov/detail/CPTC-ENTPD1-1 DB - PRIME DP - Unbound Medicine ER -