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Lens superoxide dismutase and catalase activities in diabetic cataract.
Clin Biochem. 2002 Feb; 35(1):69-72.CB

Abstract

OBJECTIVE

Biochemical evidence suggests that the oxidative damage of the lens proteins is involved in the genesis of senile cataract and the development of diabetes-related pathologic changes such as the formation of cataracts. In particular, lens proteins are subject to extensive oxidative modification. Oxidative damage either decreases the antioxidant capacity or decreased antioxidant capacity results in oxidative damage. The purpose of this study was to analyze the activities of the antioxidant enzymes such as Cu,Zn Superoxide Dismutase (Cu,Zn-SOD) and catalase in the cataractous lenses of the type 2 diabetic group and cataractous lenses of the senile group.

METHOD

Eighteen diabetic cataractous lenses and twenty six senile cataractous lenses were studied. Cu,Zn-SOD activity was measured in lenses by enzymatic method and catalase activity was measured by colorimetric method.

RESULTS

Cu,Zn-SOD levels were significantly lower in the diabetic cataractous lenses than senile cataractous lenses (respectively 8.052 +/- 0.818, 18.216 +/- 4.217 microg/g prot. p < 0.05). Similarly, catalase levels were significantly lower in the diabetic cataractous lenses than senile cataractous lenses (respectively 0.326 +/- 0.134, 0.665 +/- 0.322 kU/g prot. p < 0.001).

CONCLUSION

The results of the present study indicate that the antioxidant capacity in the diabetic cataractous lenses were decreased and this result suggests a role of antioxidant enzymes in the genesis of diabetic cataracts.

Authors+Show Affiliations

Department of Internal Medicine (Endocrinology), Celal Bayar University School of Medicine, Manisa, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11937081

Citation

Ozmen, Bilgin, et al. "Lens Superoxide Dismutase and Catalase Activities in Diabetic Cataract." Clinical Biochemistry, vol. 35, no. 1, 2002, pp. 69-72.
Ozmen B, Ozmen D, Erkin E, et al. Lens superoxide dismutase and catalase activities in diabetic cataract. Clin Biochem. 2002;35(1):69-72.
Ozmen, B., Ozmen, D., Erkin, E., Güner, I., Habif, S., & Bayindir, O. (2002). Lens superoxide dismutase and catalase activities in diabetic cataract. Clinical Biochemistry, 35(1), 69-72.
Ozmen B, et al. Lens Superoxide Dismutase and Catalase Activities in Diabetic Cataract. Clin Biochem. 2002;35(1):69-72. PubMed PMID: 11937081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lens superoxide dismutase and catalase activities in diabetic cataract. AU - Ozmen,Bilgin, AU - Ozmen,Dilek, AU - Erkin,Esin, AU - Güner,Inci, AU - Habif,Sara, AU - Bayindir,Oya, PY - 2002/4/9/pubmed PY - 2002/8/14/medline PY - 2002/4/9/entrez SP - 69 EP - 72 JF - Clinical biochemistry JO - Clin Biochem VL - 35 IS - 1 N2 - OBJECTIVE: Biochemical evidence suggests that the oxidative damage of the lens proteins is involved in the genesis of senile cataract and the development of diabetes-related pathologic changes such as the formation of cataracts. In particular, lens proteins are subject to extensive oxidative modification. Oxidative damage either decreases the antioxidant capacity or decreased antioxidant capacity results in oxidative damage. The purpose of this study was to analyze the activities of the antioxidant enzymes such as Cu,Zn Superoxide Dismutase (Cu,Zn-SOD) and catalase in the cataractous lenses of the type 2 diabetic group and cataractous lenses of the senile group. METHOD: Eighteen diabetic cataractous lenses and twenty six senile cataractous lenses were studied. Cu,Zn-SOD activity was measured in lenses by enzymatic method and catalase activity was measured by colorimetric method. RESULTS: Cu,Zn-SOD levels were significantly lower in the diabetic cataractous lenses than senile cataractous lenses (respectively 8.052 +/- 0.818, 18.216 +/- 4.217 microg/g prot. p < 0.05). Similarly, catalase levels were significantly lower in the diabetic cataractous lenses than senile cataractous lenses (respectively 0.326 +/- 0.134, 0.665 +/- 0.322 kU/g prot. p < 0.001). CONCLUSION: The results of the present study indicate that the antioxidant capacity in the diabetic cataractous lenses were decreased and this result suggests a role of antioxidant enzymes in the genesis of diabetic cataracts. SN - 0009-9120 UR - https://www.unboundmedicine.com/medline/citation/11937081/Lens_superoxide_dismutase_and_catalase_activities_in_diabetic_cataract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009912001002843 DB - PRIME DP - Unbound Medicine ER -