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Immunophenotypic variability of B-cell non-Hodgkin lymphoma: a retrospective study of cases analyzed by flow cytometry.
Am J Clin Pathol. 2002 Apr; 117(4):615-20.AJ

Abstract

Flow cytometric analysis is important in the diagnosis, classification, and follow-up of non-Hodgkin lymphoma. It is assumed that the lymphoma phenotype for each patient remains unchanged over time and is consistent from one specimen to another. To determine the variability in expression of lymphoid antigens, we reviewed 211 flow cytometry specimens of malignant lymphoma from 81 patients. Some antigens showed a stable pattern of expression such as CD5, CD10, CD19, CD20, and HLA-DR. In contrast, CD21, CD22, CD23, and CD25 showed more variability from one specimen to another. We believe several factors affect the stability of antigen expression. True differences in expression most probably are related to the biology and function of the different antigens. For instance, CD19 and CD20 are essential in cell maturation and function and, therefore, are present on the majority of cells. In contrast, CD22 has a role during B-cell activation and, therefore, is more variable. Lack of standardization inflow cytometry procedures also is responsible for some variability. Instrument settings for adequate compensation and the criteria used to determine when an antigen is reported as positive are important considerations when evaluating flow cytometry histograms.

Authors+Show Affiliations

Department of Pathology, University of Texas Health Science Center, San Antonio, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11939737

Citation

Echeverri, Carolina, et al. "Immunophenotypic Variability of B-cell non-Hodgkin Lymphoma: a Retrospective Study of Cases Analyzed By Flow Cytometry." American Journal of Clinical Pathology, vol. 117, no. 4, 2002, pp. 615-20.
Echeverri C, Fisher S, King D, et al. Immunophenotypic variability of B-cell non-Hodgkin lymphoma: a retrospective study of cases analyzed by flow cytometry. Am J Clin Pathol. 2002;117(4):615-20.
Echeverri, C., Fisher, S., King, D., & Craig, F. E. (2002). Immunophenotypic variability of B-cell non-Hodgkin lymphoma: a retrospective study of cases analyzed by flow cytometry. American Journal of Clinical Pathology, 117(4), 615-20.
Echeverri C, et al. Immunophenotypic Variability of B-cell non-Hodgkin Lymphoma: a Retrospective Study of Cases Analyzed By Flow Cytometry. Am J Clin Pathol. 2002;117(4):615-20. PubMed PMID: 11939737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunophenotypic variability of B-cell non-Hodgkin lymphoma: a retrospective study of cases analyzed by flow cytometry. AU - Echeverri,Carolina, AU - Fisher,Stephen, AU - King,David, AU - Craig,Fiona E, PY - 2002/4/10/pubmed PY - 2002/4/19/medline PY - 2002/4/10/entrez SP - 615 EP - 20 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 117 IS - 4 N2 - Flow cytometric analysis is important in the diagnosis, classification, and follow-up of non-Hodgkin lymphoma. It is assumed that the lymphoma phenotype for each patient remains unchanged over time and is consistent from one specimen to another. To determine the variability in expression of lymphoid antigens, we reviewed 211 flow cytometry specimens of malignant lymphoma from 81 patients. Some antigens showed a stable pattern of expression such as CD5, CD10, CD19, CD20, and HLA-DR. In contrast, CD21, CD22, CD23, and CD25 showed more variability from one specimen to another. We believe several factors affect the stability of antigen expression. True differences in expression most probably are related to the biology and function of the different antigens. For instance, CD19 and CD20 are essential in cell maturation and function and, therefore, are present on the majority of cells. In contrast, CD22 has a role during B-cell activation and, therefore, is more variable. Lack of standardization inflow cytometry procedures also is responsible for some variability. Instrument settings for adequate compensation and the criteria used to determine when an antigen is reported as positive are important considerations when evaluating flow cytometry histograms. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/11939737/Immunophenotypic_variability_of_B_cell_non_Hodgkin_lymphoma:_a_retrospective_study_of_cases_analyzed_by_flow_cytometry_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/AAYH-1FK8-38PL-Q6DT DB - PRIME DP - Unbound Medicine ER -