Tags

Type your tag names separated by a space and hit enter

Differential activation of nuclear factor-kappaB by tumour necrosis factor receptor subtypes. TNFR1 predominates whereas TNFR2 activates transcription poorly.
FEBS Lett. 2002 Mar 27; 515(1-3):119-26.FL

Abstract

Tumour necrosis factor-alpha (TNF-alpha) signals though two receptors, TNFR1 and TNFR2. TNFR1 has a role in cytotoxicity, whereas TNFR2 regulates death responses or proliferation. TNF activates pro-inflammatory transcription factor nuclear factor-kappaB (NF-kappaB) by uncertain signalling mechanisms. Here we report the contribution of each TNFR towards the NF-kappaB activation processes. In human cells expressing endogenous or exogenous TNFR2, in addition to TNFR1, we found both TNFRs capable of activating NF-kappaB, as measured by IkappaBalpha (inhibitor of NF-kappaB) degradation, electrophoretic mobility shift assay and NF-kappaB gene reporter assays. TNFR2 activation did not degrade IkappaBbeta. However, TNF-effects on NF-kappaB activation occurred predominantly through TNFR1, with TNFR2 activating the transcription factor poorly.

Authors+Show Affiliations

Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, AB25 2ZD, Aberdeen, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11943206

Citation

McFarlane, Shona M., et al. "Differential Activation of Nuclear factor-kappaB By Tumour Necrosis Factor Receptor Subtypes. TNFR1 Predominates Whereas TNFR2 Activates Transcription Poorly." FEBS Letters, vol. 515, no. 1-3, 2002, pp. 119-26.
McFarlane SM, Pashmi G, Connell MC, et al. Differential activation of nuclear factor-kappaB by tumour necrosis factor receptor subtypes. TNFR1 predominates whereas TNFR2 activates transcription poorly. FEBS Lett. 2002;515(1-3):119-26.
McFarlane, S. M., Pashmi, G., Connell, M. C., Littlejohn, A. F., Tucker, S. J., Vandenabeele, P., & MacEwan, D. J. (2002). Differential activation of nuclear factor-kappaB by tumour necrosis factor receptor subtypes. TNFR1 predominates whereas TNFR2 activates transcription poorly. FEBS Letters, 515(1-3), 119-26.
McFarlane SM, et al. Differential Activation of Nuclear factor-kappaB By Tumour Necrosis Factor Receptor Subtypes. TNFR1 Predominates Whereas TNFR2 Activates Transcription Poorly. FEBS Lett. 2002 Mar 27;515(1-3):119-26. PubMed PMID: 11943206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential activation of nuclear factor-kappaB by tumour necrosis factor receptor subtypes. TNFR1 predominates whereas TNFR2 activates transcription poorly. AU - McFarlane,Shona M, AU - Pashmi,Ghazaleh, AU - Connell,Michelle C, AU - Littlejohn,Alison F, AU - Tucker,Steven J, AU - Vandenabeele,Peter, AU - MacEwan,David J, PY - 2002/4/12/pubmed PY - 2002/5/11/medline PY - 2002/4/12/entrez SP - 119 EP - 26 JF - FEBS letters JO - FEBS Lett VL - 515 IS - 1-3 N2 - Tumour necrosis factor-alpha (TNF-alpha) signals though two receptors, TNFR1 and TNFR2. TNFR1 has a role in cytotoxicity, whereas TNFR2 regulates death responses or proliferation. TNF activates pro-inflammatory transcription factor nuclear factor-kappaB (NF-kappaB) by uncertain signalling mechanisms. Here we report the contribution of each TNFR towards the NF-kappaB activation processes. In human cells expressing endogenous or exogenous TNFR2, in addition to TNFR1, we found both TNFRs capable of activating NF-kappaB, as measured by IkappaBalpha (inhibitor of NF-kappaB) degradation, electrophoretic mobility shift assay and NF-kappaB gene reporter assays. TNFR2 activation did not degrade IkappaBbeta. However, TNF-effects on NF-kappaB activation occurred predominantly through TNFR1, with TNFR2 activating the transcription factor poorly. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/11943206/Differential_activation_of_nuclear_factor_kappaB_by_tumour_necrosis_factor_receptor_subtypes__TNFR1_predominates_whereas_TNFR2_activates_transcription_poorly_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S001457930202450X DB - PRIME DP - Unbound Medicine ER -