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Telomerase activity in human hepatocellular carcinoma: parallel correlation with human telomerase reverse transcriptase (hTERT) mRNA isoform expression but not with cell cycle modulators or c-Myc expression.
Eur J Surg Oncol. 2002 Apr; 28(3):225-34.EJ

Abstract

BACKGROUND

To explore the possible regulatory mechanisms of telomerase, we examined the telomerase activity (TA), expression of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTERT) mRNA isoforms and cell cycle modulators in human hepatocellular carcinoma (HCC) cell lines (J5, J7) and a normal human immortalized hepatic epithelial cell line (Chang-liver).

METHODS

The cell lines were chemically synchronized in either G1, G1/S, G2/M or M phases. TA was measured by polymerase chain reaction (PCR)-based telomerase repeat amplification protocol assay. The hTR and hTERT mRNA levels were analyzed by reverse transcriptase-polymerase chain reaction. Western blotting and immunocytochemistry were used to assay the cell cycle modulators.

RESULTS

The TA of J5, J7 and Chang-liver cell lines tested was highest in M phase. The expression level of hTERT mRNA associated with the highest TA detected in the M phase of HCC cell lines. Chang-liver expressed markedly less TA and hTERT mRNA than J5 or J7. The elevated TA and expression of hTERT mRNA isoforms in M phase of HCC cell lines did not significantly correlate with that of the cell cycle modulators and c-Myc.

CONCLUSIONS

The results implicate that regulation of TA is related to hTERT mRNA isoform expression, and that regulation is different between the cell immortalization and tumorigenesis.

Authors+Show Affiliations

Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11944954

Citation

Chen, C-J, et al. "Telomerase Activity in Human Hepatocellular Carcinoma: Parallel Correlation With Human Telomerase Reverse Transcriptase (hTERT) mRNA Isoform Expression but Not With Cell Cycle Modulators or c-Myc Expression." European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, vol. 28, no. 3, 2002, pp. 225-34.
Chen CJ, Tsai NM, Liu YC, et al. Telomerase activity in human hepatocellular carcinoma: parallel correlation with human telomerase reverse transcriptase (hTERT) mRNA isoform expression but not with cell cycle modulators or c-Myc expression. Eur J Surg Oncol. 2002;28(3):225-34.
Chen, C. J., Tsai, N. M., Liu, Y. C., Ho, L. I., Hsieh, H. F., Yen, C. Y., & Harn, H. J. (2002). Telomerase activity in human hepatocellular carcinoma: parallel correlation with human telomerase reverse transcriptase (hTERT) mRNA isoform expression but not with cell cycle modulators or c-Myc expression. European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 28(3), 225-34.
Chen CJ, et al. Telomerase Activity in Human Hepatocellular Carcinoma: Parallel Correlation With Human Telomerase Reverse Transcriptase (hTERT) mRNA Isoform Expression but Not With Cell Cycle Modulators or c-Myc Expression. Eur J Surg Oncol. 2002;28(3):225-34. PubMed PMID: 11944954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase activity in human hepatocellular carcinoma: parallel correlation with human telomerase reverse transcriptase (hTERT) mRNA isoform expression but not with cell cycle modulators or c-Myc expression. AU - Chen,C-J, AU - Tsai,N-M, AU - Liu,Y-C, AU - Ho,L I, AU - Hsieh,H-F, AU - Yen,C-Y, AU - Harn,H-J, PY - 2002/4/12/pubmed PY - 2002/5/23/medline PY - 2002/4/12/entrez SP - 225 EP - 34 JF - European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology JO - Eur J Surg Oncol VL - 28 IS - 3 N2 - BACKGROUND: To explore the possible regulatory mechanisms of telomerase, we examined the telomerase activity (TA), expression of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTERT) mRNA isoforms and cell cycle modulators in human hepatocellular carcinoma (HCC) cell lines (J5, J7) and a normal human immortalized hepatic epithelial cell line (Chang-liver). METHODS: The cell lines were chemically synchronized in either G1, G1/S, G2/M or M phases. TA was measured by polymerase chain reaction (PCR)-based telomerase repeat amplification protocol assay. The hTR and hTERT mRNA levels were analyzed by reverse transcriptase-polymerase chain reaction. Western blotting and immunocytochemistry were used to assay the cell cycle modulators. RESULTS: The TA of J5, J7 and Chang-liver cell lines tested was highest in M phase. The expression level of hTERT mRNA associated with the highest TA detected in the M phase of HCC cell lines. Chang-liver expressed markedly less TA and hTERT mRNA than J5 or J7. The elevated TA and expression of hTERT mRNA isoforms in M phase of HCC cell lines did not significantly correlate with that of the cell cycle modulators and c-Myc. CONCLUSIONS: The results implicate that regulation of TA is related to hTERT mRNA isoform expression, and that regulation is different between the cell immortalization and tumorigenesis. SN - 0748-7983 UR - https://www.unboundmedicine.com/medline/citation/11944954/Telomerase_activity_in_human_hepatocellular_carcinoma:_parallel_correlation_with_human_telomerase_reverse_transcriptase__hTERT__mRNA_isoform_expression_but_not_with_cell_cycle_modulators_or_c_Myc_expression_ DB - PRIME DP - Unbound Medicine ER -