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The effect of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor cells.
Eur Cytokine Netw. 2002 Jan-Mar; 13(1):128-33.EC

Abstract

AIMS

To determine whether granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor (NT2) cells can be regulated by IL-1beta and TNF-alpha.

BACKGROUND

We have previously demonstrated GM-CSF expression by neurons of the developing human brain, as well as by NT2 cells. IL-1beta and TNF-alpha upregulate GM-CSF production in glial cells, but GM-CSF regulation in neurons is as yet undefined. We hypothesized that IL-1beta and TNF-alpha would increase GM-CSF mRNA and protein production in NT2 cells.

METHODS

The effect of IL-1beta and TNF-alpha on GM-CSF production was assessed by dose response (0 to 2,000 U/ml), and time course (0 to 48 hours incubation) experiments. GM-CSF mRNA and protein production were assessed by quantitative RT-PCR and by ELISA. The effect of these cytokines on cell turnover was determined by BrdU incorporation.

RESULTS

IL-1beta increased GM-CSF mRNA and protein expression by NT2 cells. This effect was time and dose dependent, and the effective dose ranging from (20-200 U/ml). TNF-alpha increased GM-CSF mRNA expression to a lesser extent than did IL-1beta (maximal stimulation at 200 U/ml), and a minimal increase in net protein accumulation was noted. Neither cytokine increased NT2 cell turnover.

CONCLUSIONS

IL-1beta and TNF-alpha both increase GM-CSF mRNA expression by NT2 cells, but only IL-1beta increases net GM-CSF protein accumulation.

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Authors+Show Affiliations

Dame JB
Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL 32610, USA. john.dame@hsc.utah.edu
Chegini N
No affiliation info available
Christensen RD
No affiliation info available
Juul SE
No affiliation info available

MeSH

Cell LineCells, CulturedGranulocyte-Macrophage Colony-Stimulating FactorHumansInterleukin-1NeuronsRNA, MessengerStem CellsTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11956032

Citation

Dame, J Benjamin, et al. "The Effect of Interleukin-1beta (IL-1beta) and Tumor Necrosis Factor-alpha (TNF-alpha) On Granulocyte Macrophage-colony Stimulating Factor (GM-CSF) Production By Neuronal Precursor Cells." European Cytokine Network, vol. 13, no. 1, 2002, pp. 128-33.
Dame JB, Chegini N, Christensen RD, et al. The effect of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor cells. Eur Cytokine Netw. 2002;13(1):128-33.
Dame, J. B., Chegini, N., Christensen, R. D., & Juul, S. E. (2002). The effect of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor cells. European Cytokine Network, 13(1), 128-33.
Dame JB, et al. The Effect of Interleukin-1beta (IL-1beta) and Tumor Necrosis Factor-alpha (TNF-alpha) On Granulocyte Macrophage-colony Stimulating Factor (GM-CSF) Production By Neuronal Precursor Cells. Eur Cytokine Netw. 2002 Jan-Mar;13(1):128-33. PubMed PMID: 11956032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor cells. AU - Dame,J Benjamin, AU - Chegini,Nasser, AU - Christensen,Robert D, AU - Juul,Sandra E, PY - 2002/4/17/pubmed PY - 2002/9/27/medline PY - 2002/4/17/entrez SP - 128 EP - 33 JF - European cytokine network JO - Eur Cytokine Netw VL - 13 IS - 1 N2 - AIMS: To determine whether granulocyte macrophage-colony stimulating factor (GM-CSF) production by neuronal precursor (NT2) cells can be regulated by IL-1beta and TNF-alpha. BACKGROUND: We have previously demonstrated GM-CSF expression by neurons of the developing human brain, as well as by NT2 cells. IL-1beta and TNF-alpha upregulate GM-CSF production in glial cells, but GM-CSF regulation in neurons is as yet undefined. We hypothesized that IL-1beta and TNF-alpha would increase GM-CSF mRNA and protein production in NT2 cells. METHODS: The effect of IL-1beta and TNF-alpha on GM-CSF production was assessed by dose response (0 to 2,000 U/ml), and time course (0 to 48 hours incubation) experiments. GM-CSF mRNA and protein production were assessed by quantitative RT-PCR and by ELISA. The effect of these cytokines on cell turnover was determined by BrdU incorporation. RESULTS: IL-1beta increased GM-CSF mRNA and protein expression by NT2 cells. This effect was time and dose dependent, and the effective dose ranging from (20-200 U/ml). TNF-alpha increased GM-CSF mRNA expression to a lesser extent than did IL-1beta (maximal stimulation at 200 U/ml), and a minimal increase in net protein accumulation was noted. Neither cytokine increased NT2 cell turnover. CONCLUSIONS: IL-1beta and TNF-alpha both increase GM-CSF mRNA expression by NT2 cells, but only IL-1beta increases net GM-CSF protein accumulation. SN - 1148-5493 UR - https://www.unboundmedicine.com/medline/citation/11956032/The_effect_of_interleukin_1beta__IL_1beta__and_tumor_necrosis_factor_alpha__TNF_alpha__on_granulocyte_macrophage_colony_stimulating_factor__GM_CSF__production_by_neuronal_precursor_cells_ L2 - http://www.jle.com/medline.md?issn=1148-5493&vol=13&iss=1&page=128 DB - PRIME DP - Unbound Medicine ER -
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