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Influence of aeration, storage, and rinsing conditions on residual ethylene oxide in freeze-dried bone allograft.
J Orthop Sci 2002; 7(2):238-42JO

Abstract

Bone allografts sterilized with ethylene oxide gas (EO) are used in the field of orthopedic surgery, and the reduction of the EO residual concentration is an urgent clinical matter. We therefore investigated the efficacy of aeration and the effects of varied preservation periods and rinsing conditions on the reduction of EO residuals in freeze-dried bone allografts in the present study. Before aeration, the EO residual level was 12.6 ppm, and, after the repeating of aeration at 60 degrees C once, two times, and three times, the level decreased to 10.9 ppm, 3.1 ppm, and 0.47 ppm, respectively. Regarding the duration of preservation at room temperature, the mean EO residual level was 10.5 ppm, 4.9 ppm, and 4.6 ppm, 1, 2, and 3 weeks after EO sterilization, respectively. By rinsing with physiological salt solution, the level was decreased to 6.9 ppm by 5-min rinsing with 100 ml. Rinsing with 500 ml of this solution decreased the levels to 3.9 ppm, 2.8 ppm, and 2.0 ppm when done for 1, 5, and 10 min, respectively. Rinsing with 2000 ml of this solution decreased the levels to 3.6 ppm, 2.6 ppm, and 1.7 ppm when done for 1, 5, and 10 min, respectively. These experimental results with chip bone allografts lead us to recommend repeated preoperative aeration and more than 2 weeks' preservation before use for reducing the residual EO concentration. It was also evident that intraoperative rinsing with 500 ml of physiological saline for 10 min reduced the EO residual level.

Authors+Show Affiliations

Department of Orthopedic Surgery, Faculty of Medicine, Oita Medical University, Hasama-machi, Oita 879-5593, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11956985

Citation

Kaku, Nobuhiro, et al. "Influence of Aeration, Storage, and Rinsing Conditions On Residual Ethylene Oxide in Freeze-dried Bone Allograft." Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association, vol. 7, no. 2, 2002, pp. 238-42.
Kaku N, Tsumura H, Kataoka M, et al. Influence of aeration, storage, and rinsing conditions on residual ethylene oxide in freeze-dried bone allograft. J Orthop Sci. 2002;7(2):238-42.
Kaku, N., Tsumura, H., Kataoka, M., Taira, H., & Torisu, T. (2002). Influence of aeration, storage, and rinsing conditions on residual ethylene oxide in freeze-dried bone allograft. Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association, 7(2), pp. 238-42.
Kaku N, et al. Influence of Aeration, Storage, and Rinsing Conditions On Residual Ethylene Oxide in Freeze-dried Bone Allograft. J Orthop Sci. 2002;7(2):238-42. PubMed PMID: 11956985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of aeration, storage, and rinsing conditions on residual ethylene oxide in freeze-dried bone allograft. AU - Kaku,Nobuhiro, AU - Tsumura,Hiroshi, AU - Kataoka,Masashi, AU - Taira,Hirofumi, AU - Torisu,Takehiko, PY - 2002/4/17/pubmed PY - 2002/5/29/medline PY - 2002/4/17/entrez SP - 238 EP - 42 JF - Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association JO - J Orthop Sci VL - 7 IS - 2 N2 - Bone allografts sterilized with ethylene oxide gas (EO) are used in the field of orthopedic surgery, and the reduction of the EO residual concentration is an urgent clinical matter. We therefore investigated the efficacy of aeration and the effects of varied preservation periods and rinsing conditions on the reduction of EO residuals in freeze-dried bone allografts in the present study. Before aeration, the EO residual level was 12.6 ppm, and, after the repeating of aeration at 60 degrees C once, two times, and three times, the level decreased to 10.9 ppm, 3.1 ppm, and 0.47 ppm, respectively. Regarding the duration of preservation at room temperature, the mean EO residual level was 10.5 ppm, 4.9 ppm, and 4.6 ppm, 1, 2, and 3 weeks after EO sterilization, respectively. By rinsing with physiological salt solution, the level was decreased to 6.9 ppm by 5-min rinsing with 100 ml. Rinsing with 500 ml of this solution decreased the levels to 3.9 ppm, 2.8 ppm, and 2.0 ppm when done for 1, 5, and 10 min, respectively. Rinsing with 2000 ml of this solution decreased the levels to 3.6 ppm, 2.6 ppm, and 1.7 ppm when done for 1, 5, and 10 min, respectively. These experimental results with chip bone allografts lead us to recommend repeated preoperative aeration and more than 2 weeks' preservation before use for reducing the residual EO concentration. It was also evident that intraoperative rinsing with 500 ml of physiological saline for 10 min reduced the EO residual level. SN - 0949-2658 UR - https://www.unboundmedicine.com/medline/citation/11956985/Influence_of_aeration_storage_and_rinsing_conditions_on_residual_ethylene_oxide_in_freeze_dried_bone_allograft_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0949-2658(15)33227-9 DB - PRIME DP - Unbound Medicine ER -