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Glucose or essential amino acid infusions in late pregnant and early lactating Simmenthal cows failed to induce a leptin response.

Abstract

To assess the leptin response to metabolic challenges, three Italian Simmental cows were infused for 6 h: with (a) saline (control); (b) glucose; and (c) amino acid solutions according to a 3 x 3 Latin square experiment. The infusions were carried out at the 36th week of pregnancy, and the second and 12th week of the following lactation. At each of the three infusion periods, blood samples were collected from the jugular vein before and 15, 30, 120, 180, 240, 300 and 360 min after the beginning of each infusion. All samples were analysed for leptin, insulin, glucagon, growth hormone (GH), glucose, non-esterified fatty acids (NEFA) and urea. The physiological phase of the cows significantly affected the basal concentrations of insulin, glucagon, urea and NEFA. The infusion of both glucose and the amino acid solutions did not affect leptin concentrations. Insulin response was significantly increased when animals were infused with the glucose solution and, within treatment, the greatest response was observed at the 12th week of lactation. The greatest glucagon response was observed when infusing the amino acid solution. Urea response to all treatments increased from the dry period to the 12th week of lactation. The GH and NEFA responses were not affected by treatments. The Multi Species radio-immunoassay used in this study showed a lower sensitivity for ruminant leptin which may partially explain the lack of significant leptin variations. However, it can be hypothesized that leptin variations around parturition can be affected by the negative energy balance, and leptin release is not acutely affected by glucose and amino acid availability. In addition, no short-term relationship were found between insulin, glucagon and GH and leptin release in Italian Simmental cows during the dry period and early lactation.

Authors+Show Affiliations

Dipartimento di Scienze Zootecniche, Facoltà di Medicina Veterinaria, Università di Padova, Agripolis, Legnaro (PD), Italy. gianfranco.gabai@unipd.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11958470

Citation

Gabai, G, et al. "Glucose or Essential Amino Acid Infusions in Late Pregnant and Early Lactating Simmenthal Cows Failed to Induce a Leptin Response." Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, vol. 49, no. 2, 2002, pp. 73-80.
Gabai G, Cozzi G, Rosi F, et al. Glucose or essential amino acid infusions in late pregnant and early lactating Simmenthal cows failed to induce a leptin response. J Vet Med A Physiol Pathol Clin Med. 2002;49(2):73-80.
Gabai, G., Cozzi, G., Rosi, F., Andrighetto, I., & Bono, G. (2002). Glucose or essential amino acid infusions in late pregnant and early lactating Simmenthal cows failed to induce a leptin response. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, 49(2), pp. 73-80.
Gabai G, et al. Glucose or Essential Amino Acid Infusions in Late Pregnant and Early Lactating Simmenthal Cows Failed to Induce a Leptin Response. J Vet Med A Physiol Pathol Clin Med. 2002;49(2):73-80. PubMed PMID: 11958470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucose or essential amino acid infusions in late pregnant and early lactating Simmenthal cows failed to induce a leptin response. AU - Gabai,G, AU - Cozzi,G, AU - Rosi,F, AU - Andrighetto,I, AU - Bono,G, PY - 2002/4/18/pubmed PY - 2002/5/17/medline PY - 2002/4/18/entrez SP - 73 EP - 80 JF - Journal of veterinary medicine. A, Physiology, pathology, clinical medicine JO - J Vet Med A Physiol Pathol Clin Med VL - 49 IS - 2 N2 - To assess the leptin response to metabolic challenges, three Italian Simmental cows were infused for 6 h: with (a) saline (control); (b) glucose; and (c) amino acid solutions according to a 3 x 3 Latin square experiment. The infusions were carried out at the 36th week of pregnancy, and the second and 12th week of the following lactation. At each of the three infusion periods, blood samples were collected from the jugular vein before and 15, 30, 120, 180, 240, 300 and 360 min after the beginning of each infusion. All samples were analysed for leptin, insulin, glucagon, growth hormone (GH), glucose, non-esterified fatty acids (NEFA) and urea. The physiological phase of the cows significantly affected the basal concentrations of insulin, glucagon, urea and NEFA. The infusion of both glucose and the amino acid solutions did not affect leptin concentrations. Insulin response was significantly increased when animals were infused with the glucose solution and, within treatment, the greatest response was observed at the 12th week of lactation. The greatest glucagon response was observed when infusing the amino acid solution. Urea response to all treatments increased from the dry period to the 12th week of lactation. The GH and NEFA responses were not affected by treatments. The Multi Species radio-immunoassay used in this study showed a lower sensitivity for ruminant leptin which may partially explain the lack of significant leptin variations. However, it can be hypothesized that leptin variations around parturition can be affected by the negative energy balance, and leptin release is not acutely affected by glucose and amino acid availability. In addition, no short-term relationship were found between insulin, glucagon and GH and leptin release in Italian Simmental cows during the dry period and early lactation. SN - 0931-184X UR - https://www.unboundmedicine.com/medline/citation/11958470/Glucose_or_essential_amino_acid_infusions_in_late_pregnant_and_early_lactating_Simmenthal_cows_failed_to_induce_a_leptin_response_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0931-184X&date=2002&volume=49&issue=2&spage=73 DB - PRIME DP - Unbound Medicine ER -