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Delayed examination of synovial fluid by ordinary and polarised light microscopy to detect and identify crystals.
Ann Rheum Dis. 2002 May; 61(5):444-7.AR

Abstract

OBJECTIVE

To determine the reliability of a delay in the microscopic examination of synovial fluid (SF) to detect and identify crystals.

METHODS

Ninety one SF samples were examined, 31 with monosodium urate (MSU) crystals, 30 with crystals of calcium pyrophosphate dihydrate (CPPD), and 30 containing no crystals. The specimens were stored with EDTA, sodium heparin, and without anticoagulant at 4 degrees C before examination at 24 and 72 hours with ordinary and polarised light microscopy. Another aliquot of the same samples was stored in a plastic container without anticoagulant at -80 degrees C and examined after two months.

RESULTS

When the samples stored at 4 degrees C were re-examined after 24 hours, intracellular crystals of MSU were seen in 90/93 (97%) cases where they had been identified previously and 89/93 (96%) cases after 72 hours. Similarly, CPPD crystals were identified in 90/90 (100%) and 87/90 (97%) cases after 24 and 72 hours. Examination of the samples stored at -80 degrees C showed intracellular MSU crystals in 25/31 (81%) of cases and CPPD crystals in 25/30 (83%). No crystals were seen in any sample which had previously been diagnosed as crystal-free.

CONCLUSIONS

Deferred microscopic examination of refrigerated or deep frozen SF provides a strong probability of detecting MSU or CPPD crystals if these are present initially.

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Authors+Show Affiliations

Gálvez J
Rheumatology Unit, Hospital General Universitario "JM Morales Meseguer", Murcia, Spain. pgalvis@eresmas.com
Sáiz E
No affiliation info available
Linares LF
No affiliation info available
Climent A
No affiliation info available
Marras C
No affiliation info available
Pina MF
No affiliation info available
Castellón P
No affiliation info available

MeSH

Calcium PyrophosphateCase-Control StudiesCryopreservationCrystallizationCulture MediaEdetic AcidGoutHeparinHumansSynovial FluidTime FactorsUric Acid

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11959769

Citation

Gálvez, J, et al. "Delayed Examination of Synovial Fluid By Ordinary and Polarised Light Microscopy to Detect and Identify Crystals." Annals of the Rheumatic Diseases, vol. 61, no. 5, 2002, pp. 444-7.
Gálvez J, Sáiz E, Linares LF, et al. Delayed examination of synovial fluid by ordinary and polarised light microscopy to detect and identify crystals. Ann Rheum Dis. 2002;61(5):444-7.
Gálvez, J., Sáiz, E., Linares, L. F., Climent, A., Marras, C., Pina, M. F., & Castellón, P. (2002). Delayed examination of synovial fluid by ordinary and polarised light microscopy to detect and identify crystals. Annals of the Rheumatic Diseases, 61(5), 444-7.
Gálvez J, et al. Delayed Examination of Synovial Fluid By Ordinary and Polarised Light Microscopy to Detect and Identify Crystals. Ann Rheum Dis. 2002;61(5):444-7. PubMed PMID: 11959769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Delayed examination of synovial fluid by ordinary and polarised light microscopy to detect and identify crystals. AU - Gálvez,J, AU - Sáiz,E, AU - Linares,L F, AU - Climent,A, AU - Marras,C, AU - Pina,M F, AU - Castellón,P, PY - 2002/4/18/pubmed PY - 2002/6/14/medline PY - 2002/4/18/entrez SP - 444 EP - 7 JF - Annals of the rheumatic diseases JO - Ann Rheum Dis VL - 61 IS - 5 N2 - OBJECTIVE: To determine the reliability of a delay in the microscopic examination of synovial fluid (SF) to detect and identify crystals. METHODS: Ninety one SF samples were examined, 31 with monosodium urate (MSU) crystals, 30 with crystals of calcium pyrophosphate dihydrate (CPPD), and 30 containing no crystals. The specimens were stored with EDTA, sodium heparin, and without anticoagulant at 4 degrees C before examination at 24 and 72 hours with ordinary and polarised light microscopy. Another aliquot of the same samples was stored in a plastic container without anticoagulant at -80 degrees C and examined after two months. RESULTS: When the samples stored at 4 degrees C were re-examined after 24 hours, intracellular crystals of MSU were seen in 90/93 (97%) cases where they had been identified previously and 89/93 (96%) cases after 72 hours. Similarly, CPPD crystals were identified in 90/90 (100%) and 87/90 (97%) cases after 24 and 72 hours. Examination of the samples stored at -80 degrees C showed intracellular MSU crystals in 25/31 (81%) of cases and CPPD crystals in 25/30 (83%). No crystals were seen in any sample which had previously been diagnosed as crystal-free. CONCLUSIONS: Deferred microscopic examination of refrigerated or deep frozen SF provides a strong probability of detecting MSU or CPPD crystals if these are present initially. SN - 0003-4967 UR - https://www.unboundmedicine.com/medline/citation/11959769/Delayed_examination_of_synovial_fluid_by_ordinary_and_polarised_light_microscopy_to_detect_and_identify_crystals_ L2 - https://ard.bmj.com/lookup/pmidlookup?view=long&pmid=11959769 DB - PRIME DP - Unbound Medicine ER -