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Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways.
Aliment Pharmacol Ther. 2002 Apr; 16 Suppl 2:107-14.AP

Abstract

BACKGROUND & AIMS

Alcoholic beverages such as beer and wine are well known to potently stimulate gastric acid secretion, most probably through an increase in circulating gastrin level. The present study examined whether or not wine stimulates gastric acid secretion by a direct effect on parietal cells, enterochromaffin-like (ECL) cells or both.

METHODS

Gastric mucosa was isolated from female Japanese white rabbits and gland specimens were prepared by the collagenase digestion method. Acid secretion was assessed by gland accumulation of [14C] aminopyrine. The effects of red wine, ethanol, non-alcoholic wine and drugs were determined by incubating gastric glands with aminopyrine. Radioactivity in solubilized glands was determined by a liquid scintillation counting.

RESULTS

Neither wine nor ethanol (diluted 1 : 10(2) to 1 : 10(4)) had any effect on gastric acid secretion, whereas non-alcoholic wine stimulated acid secretion in a dose-dependent manner. All substances, however, significantly stimulated gastric acid secretion in IBMX (phosphodiesterase inhibitor)-pretreated glands. S-0509 (a CCK-2 receptor antagonist) and atropine had no effect on acid secretion stimulated by wine, ethanol or non-alcoholic wine in IBMX-pretreated glands. Famotidine and omeprazole significantly inhibited the acid secretion resulting from all of the above stimulants. BAPTA (an intracellular Ca2+ chelator) inhibited acid secretion stimulated with wine or ethanol in a dose-dependent manner, but did not inhibit secretion stimulated by non-alcoholic wine.

CONCLUSIONS

Wine was found to stimulate gastric acid secretion in gastric glands via two pathways, by an ethanol-induced increase in the concentration of intracellular Ca2+ in parietal cells, and by histamine release from ECL cells potentially induced by constituents present in wine.

Authors+Show Affiliations

Department of Applied Pharmacology, Kyoto Pharmaceutical University, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11966531

Citation

Matsuno, K, et al. "Wine Stimulates Gastric Acid Secretion in Isolated Rabbit Gastric Glands Via Two Different Pathways." Alimentary Pharmacology & Therapeutics, vol. 16 Suppl 2, 2002, pp. 107-14.
Matsuno K, Tomita K, Okabe S. Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways. Aliment Pharmacol Ther. 2002;16 Suppl 2:107-14.
Matsuno, K., Tomita, K., & Okabe, S. (2002). Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways. Alimentary Pharmacology & Therapeutics, 16 Suppl 2, 107-14.
Matsuno K, Tomita K, Okabe S. Wine Stimulates Gastric Acid Secretion in Isolated Rabbit Gastric Glands Via Two Different Pathways. Aliment Pharmacol Ther. 2002;16 Suppl 2:107-14. PubMed PMID: 11966531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways. AU - Matsuno,K, AU - Tomita,K, AU - Okabe,S, PY - 2002/4/23/pubmed PY - 2002/7/16/medline PY - 2002/4/23/entrez SP - 107 EP - 14 JF - Alimentary pharmacology & therapeutics JO - Aliment Pharmacol Ther VL - 16 Suppl 2 N2 - BACKGROUND & AIMS: Alcoholic beverages such as beer and wine are well known to potently stimulate gastric acid secretion, most probably through an increase in circulating gastrin level. The present study examined whether or not wine stimulates gastric acid secretion by a direct effect on parietal cells, enterochromaffin-like (ECL) cells or both. METHODS: Gastric mucosa was isolated from female Japanese white rabbits and gland specimens were prepared by the collagenase digestion method. Acid secretion was assessed by gland accumulation of [14C] aminopyrine. The effects of red wine, ethanol, non-alcoholic wine and drugs were determined by incubating gastric glands with aminopyrine. Radioactivity in solubilized glands was determined by a liquid scintillation counting. RESULTS: Neither wine nor ethanol (diluted 1 : 10(2) to 1 : 10(4)) had any effect on gastric acid secretion, whereas non-alcoholic wine stimulated acid secretion in a dose-dependent manner. All substances, however, significantly stimulated gastric acid secretion in IBMX (phosphodiesterase inhibitor)-pretreated glands. S-0509 (a CCK-2 receptor antagonist) and atropine had no effect on acid secretion stimulated by wine, ethanol or non-alcoholic wine in IBMX-pretreated glands. Famotidine and omeprazole significantly inhibited the acid secretion resulting from all of the above stimulants. BAPTA (an intracellular Ca2+ chelator) inhibited acid secretion stimulated with wine or ethanol in a dose-dependent manner, but did not inhibit secretion stimulated by non-alcoholic wine. CONCLUSIONS: Wine was found to stimulate gastric acid secretion in gastric glands via two pathways, by an ethanol-induced increase in the concentration of intracellular Ca2+ in parietal cells, and by histamine release from ECL cells potentially induced by constituents present in wine. SN - 0269-2813 UR - https://www.unboundmedicine.com/medline/citation/11966531/Wine_stimulates_gastric_acid_secretion_in_isolated_rabbit_gastric_glands_via_two_different_pathways_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0269-2813&date=2002&volume=16&issue=&spage=107 DB - PRIME DP - Unbound Medicine ER -