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alpha-Phenyl-N-tert-butylnitrone provides protection from dextran sulfate sodium-induced colitis in mice.
Antioxid Redox Signal. 2002 Feb; 4(1):195-206.AR

Abstract

Nuclear factor-kappaB (NF-kappaB)-dependent up-regulation of inflammatory cytokines and inducible nitric oxide (iNOS) occurs in inflammatory bowel disease. We investigated the effect of alpha-phenylN-tert-butylnitrone (PBN), a spin-trapping agent that inhibits NF-kappaB activity, on dextran sulfate sodium (DSS)-induced colonic mucosal injury and inflammation in mice. Acute colitis was induced with DSS in female BALB/c mice receiving 0, 0.3, 3, and 30 mg/kg i.p. PBN daily. Colonic mucosal inflammation was evaluated biochemically and histologically. Nitric oxide was evaluated as luminal nitrite/nitrite concentration by the Griess reaction and as immunoreactive nitrotyrosine in mucosal cells. Mucosal tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) were determined by immunoassay. Colonic mRNA expression for iNOS, TNF-alpha, and IFN-gamma was measured by reverse transcription-polymerase chain reaction, and NF-kappaB activation was evaluated by electrophoretic mobility shift assay. After DSS administration, mice showed increased luminal nitrite/nitrate, mucosal TNF-alpha and IFN-gamma, and mRNA for iNOS and these cytokines, in addition to decreased colonic length and increased inflammatory score, luminal hemoglobin, and colonic myeloperoxidase activity. PBN inhibited increases in luminal nitric oxide production, nitrotyrosine immunoreactivity, and mucosal TNF-alpha and IFN-gamma. Colonic iNOS, TNF-alpha, and IFN-gamma mRNA were suppressed by PBN, as was a DSS-induced increase in colonic NF-kappaB DNA-binding activity. NF-kappaB is essential to DSS-induced colitis, suggesting molecular approach targeting of NF-kappaB for treatment of inflammatory bowel disease.

Authors+Show Affiliations

First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. ynaito@koto.kpu-m.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11970853

Citation

Naito, Yuji, et al. "Alpha-Phenyl-N-tert-butylnitrone Provides Protection From Dextran Sulfate Sodium-induced Colitis in Mice." Antioxidants & Redox Signaling, vol. 4, no. 1, 2002, pp. 195-206.
Naito Y, Takagi T, Ishikawa T, et al. Alpha-Phenyl-N-tert-butylnitrone provides protection from dextran sulfate sodium-induced colitis in mice. Antioxid Redox Signal. 2002;4(1):195-206.
Naito, Y., Takagi, T., Ishikawa, T., Handa, O., Matsumoto, N., Yagi, N., Matsuyama, K., Yoshida, N., Yoshikawa, T., & Kotake, Y. (2002). Alpha-Phenyl-N-tert-butylnitrone provides protection from dextran sulfate sodium-induced colitis in mice. Antioxidants & Redox Signaling, 4(1), 195-206.
Naito Y, et al. Alpha-Phenyl-N-tert-butylnitrone Provides Protection From Dextran Sulfate Sodium-induced Colitis in Mice. Antioxid Redox Signal. 2002;4(1):195-206. PubMed PMID: 11970853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - alpha-Phenyl-N-tert-butylnitrone provides protection from dextran sulfate sodium-induced colitis in mice. AU - Naito,Yuji, AU - Takagi,Tomohisa, AU - Ishikawa,Takeshi, AU - Handa,Osamu, AU - Matsumoto,Naoyuki, AU - Yagi,Nobuaki, AU - Matsuyama,Kiichi, AU - Yoshida,Norimasa, AU - Yoshikawa,Toshikazu, AU - Kotake,Yashige, PY - 2002/4/24/pubmed PY - 2002/8/13/medline PY - 2002/4/24/entrez SP - 195 EP - 206 JF - Antioxidants & redox signaling JO - Antioxid. Redox Signal. VL - 4 IS - 1 N2 - Nuclear factor-kappaB (NF-kappaB)-dependent up-regulation of inflammatory cytokines and inducible nitric oxide (iNOS) occurs in inflammatory bowel disease. We investigated the effect of alpha-phenylN-tert-butylnitrone (PBN), a spin-trapping agent that inhibits NF-kappaB activity, on dextran sulfate sodium (DSS)-induced colonic mucosal injury and inflammation in mice. Acute colitis was induced with DSS in female BALB/c mice receiving 0, 0.3, 3, and 30 mg/kg i.p. PBN daily. Colonic mucosal inflammation was evaluated biochemically and histologically. Nitric oxide was evaluated as luminal nitrite/nitrite concentration by the Griess reaction and as immunoreactive nitrotyrosine in mucosal cells. Mucosal tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) were determined by immunoassay. Colonic mRNA expression for iNOS, TNF-alpha, and IFN-gamma was measured by reverse transcription-polymerase chain reaction, and NF-kappaB activation was evaluated by electrophoretic mobility shift assay. After DSS administration, mice showed increased luminal nitrite/nitrate, mucosal TNF-alpha and IFN-gamma, and mRNA for iNOS and these cytokines, in addition to decreased colonic length and increased inflammatory score, luminal hemoglobin, and colonic myeloperoxidase activity. PBN inhibited increases in luminal nitric oxide production, nitrotyrosine immunoreactivity, and mucosal TNF-alpha and IFN-gamma. Colonic iNOS, TNF-alpha, and IFN-gamma mRNA were suppressed by PBN, as was a DSS-induced increase in colonic NF-kappaB DNA-binding activity. NF-kappaB is essential to DSS-induced colitis, suggesting molecular approach targeting of NF-kappaB for treatment of inflammatory bowel disease. SN - 1523-0864 UR - https://www.unboundmedicine.com/medline/citation/11970853/alpha_Phenyl_N_tert_butylnitrone_provides_protection_from_dextran_sulfate_sodium_induced_colitis_in_mice_ L2 - https://www.liebertpub.com/doi/full/10.1089/152308602753625951?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -