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[Helicobacter pylori genotypes in non atrophic gastritis are different of the found in peptic ulcer, premalignant lesions and gastric cancer in Colombia].
Rev Med Chil. 2002 Feb; 130(2):143-51.RM

Abstract

BACKGROUND

Helicobacter pylori is recognized as an etiologic agent of several gastric diseases. Bacterial genotypes have been related to clinical outcome in several populations.

AIM

To compare cagA, vacA and iceA genotypes of Colombian isolates from patients with several gastrointestinal diseases, including gastric cancer.

MATERIAL AND METHODS

We used polymerase chain reactions to amplify vacA, cagA and iceA genes of 137 H pylori isolates coming from 26 patients with gastric cancer (GC), 34 with peptic ulcer (PU), 19 with intestinal metaplasia (IM), 23 with atrophic gastritis (AG) and 35 with non atrophic gastritis (NAG).

RESULTS

vacA s1-m1, cagA+, iceA+ were the most frequently found genotypes. vacA s1 and m1 subtypes were found in 92 (67%) and 82 (60%) cases respectively. Sixty three percent were cagA+ and 85% were iceA+. There was a lower prevalence of s1 allele in cases of NAG (43%), compared with GC, PU and IM (81%, 77% and 81% prevalence, respectively, p < 0.01). Isolates from NAG also showed a low frequency of vacA m1 subtype (40%) compared with GC or IM (81% and 84% respectively, p < 0.01). The prevalence of cagA+ strains was significantly higher in GC patients (80%) than in NAG patients (51.4%, p < 0.01). No differences in the frequency of vacA s1a, s1b and iceA subtypes, were observed.

CONCLUSIONS

A lower frequency of cytotoxic H pylori genotypes such as cagA and vacA s1m1 and a higher frequency of non cytotoxic genotypes, was observed in patients with NAG, when compared to patients with GC or PU. These results suggest that even in Colombia, vacA and cagA could be used as markers of increased virulence.

Authors+Show Affiliations

Laboratorio de Inmunología, Grupo de Gastroenterología y de Epidemiología, Instituto Nacional de Cancerología, Bogotá, Colombia, Unidad de Gastroenterología, Hospital Universitario La Samaritana, Bogotá, Colombia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

11974526

Citation

Cittelly, Diana M., et al. "[Helicobacter Pylori Genotypes in Non Atrophic Gastritis Are Different of the Found in Peptic Ulcer, Premalignant Lesions and Gastric Cancer in Colombia]." Revista Medica De Chile, vol. 130, no. 2, 2002, pp. 143-51.
Cittelly DM, Huertas MG, Martínez JD, et al. [Helicobacter pylori genotypes in non atrophic gastritis are different of the found in peptic ulcer, premalignant lesions and gastric cancer in Colombia]. Rev Med Chil. 2002;130(2):143-51.
Cittelly, D. M., Huertas, M. G., Martínez, J. D., Oliveros, R., Posso, H., Bravo, M. M., & Orozco, O. (2002). [Helicobacter pylori genotypes in non atrophic gastritis are different of the found in peptic ulcer, premalignant lesions and gastric cancer in Colombia]. Revista Medica De Chile, 130(2), 143-51.
Cittelly DM, et al. [Helicobacter Pylori Genotypes in Non Atrophic Gastritis Are Different of the Found in Peptic Ulcer, Premalignant Lesions and Gastric Cancer in Colombia]. Rev Med Chil. 2002;130(2):143-51. PubMed PMID: 11974526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Helicobacter pylori genotypes in non atrophic gastritis are different of the found in peptic ulcer, premalignant lesions and gastric cancer in Colombia]. AU - Cittelly,Diana M, AU - Huertas,Mónica G, AU - Martínez,Julián D, AU - Oliveros,Ricardo, AU - Posso,Héctor, AU - Bravo,María Mercedes, AU - Orozco,Oscar, PY - 2002/4/27/pubmed PY - 2002/5/30/medline PY - 2002/4/27/entrez SP - 143 EP - 51 JF - Revista medica de Chile JO - Rev Med Chil VL - 130 IS - 2 N2 - BACKGROUND: Helicobacter pylori is recognized as an etiologic agent of several gastric diseases. Bacterial genotypes have been related to clinical outcome in several populations. AIM: To compare cagA, vacA and iceA genotypes of Colombian isolates from patients with several gastrointestinal diseases, including gastric cancer. MATERIAL AND METHODS: We used polymerase chain reactions to amplify vacA, cagA and iceA genes of 137 H pylori isolates coming from 26 patients with gastric cancer (GC), 34 with peptic ulcer (PU), 19 with intestinal metaplasia (IM), 23 with atrophic gastritis (AG) and 35 with non atrophic gastritis (NAG). RESULTS: vacA s1-m1, cagA+, iceA+ were the most frequently found genotypes. vacA s1 and m1 subtypes were found in 92 (67%) and 82 (60%) cases respectively. Sixty three percent were cagA+ and 85% were iceA+. There was a lower prevalence of s1 allele in cases of NAG (43%), compared with GC, PU and IM (81%, 77% and 81% prevalence, respectively, p < 0.01). Isolates from NAG also showed a low frequency of vacA m1 subtype (40%) compared with GC or IM (81% and 84% respectively, p < 0.01). The prevalence of cagA+ strains was significantly higher in GC patients (80%) than in NAG patients (51.4%, p < 0.01). No differences in the frequency of vacA s1a, s1b and iceA subtypes, were observed. CONCLUSIONS: A lower frequency of cytotoxic H pylori genotypes such as cagA and vacA s1m1 and a higher frequency of non cytotoxic genotypes, was observed in patients with NAG, when compared to patients with GC or PU. These results suggest that even in Colombia, vacA and cagA could be used as markers of increased virulence. SN - 0034-9887 UR - https://www.unboundmedicine.com/medline/citation/11974526/[Helicobacter_pylori_genotypes_in_non_atrophic_gastritis_are_different_of_the_found_in_peptic_ulcer_premalignant_lesions_and_gastric_cancer_in_Colombia]_ L2 - https://medlineplus.gov/pepticulcer.html DB - PRIME DP - Unbound Medicine ER -