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Monitoring of cytomegalovirus reactivation after allogeneic stem cell transplantation: comparison of an antigenemia assay and quantitative real-time polymerase chain reaction.
Bone Marrow Transplant. 2002 Apr; 29(7):599-606.BM

Abstract

Cytomegalovirus (CMV) antigenemia and quantitative real-time polymerase chain reaction (PCR) were compared for monitoring of CMV reactivation after allogeneic stem cell transplantation. The number of CMV antigen-positive cells by the antigenemia assay and the level of CMV DNA by real-time PCR correlated well. The sensitivity and specificity of the antigenemia assay was 55.4% and 95.5%, respectively, using real-time PCR as the reference standard. The probability of positive antigenemia at day 100 was 76.5%, with a median of first detection at day 37 in 51 patients, compared with a positive PCR of 84.3% and day 33, respectively. When HLA-identical sibling donor transplant recipients and other donor transplant recipients were analyzed separately, there was no difference between the two tests. However, temporal patterns of first detection of CMV antigen-positive cells and CMV DNA differed between HLA-identical and alternative recipients; patients without CMV (29%) or with sporadic positive PCR results (14%) were more common in HLA-identical sibling transplants, whereas patients with simultaneous antigenemia and positive PCR occurred more in alternative transplants (48%). Two of 51 patients (4%) developed CMV colitis despite antigenemia-guided prophylaxis, but both were successfully treated with ganciclovir. Although PCR is more sensitive than antigenemia, both tests are useful in the early detection of CMV after allogeneic stem cell transplantation.

Authors+Show Affiliations

Second Department of Internal Medicine, Kurume University School of Medicine, Kurume, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11979310

Citation

Yakushiji, K, et al. "Monitoring of Cytomegalovirus Reactivation After Allogeneic Stem Cell Transplantation: Comparison of an Antigenemia Assay and Quantitative Real-time Polymerase Chain Reaction." Bone Marrow Transplantation, vol. 29, no. 7, 2002, pp. 599-606.
Yakushiji K, Gondo H, Kamezaki K, et al. Monitoring of cytomegalovirus reactivation after allogeneic stem cell transplantation: comparison of an antigenemia assay and quantitative real-time polymerase chain reaction. Bone Marrow Transplant. 2002;29(7):599-606.
Yakushiji, K., Gondo, H., Kamezaki, K., Shigematsu, K., Hayashi, S., Kuroiwa, M., Taniguchi, S., Ohno, Y., Takase, K., Numata, A., Aoki, K., Kato, K., Nagafuji, K., Shimoda, K., Okamura, T., Kinukawa, N., Kasuga, N., Sata, M., & Harada, M. (2002). Monitoring of cytomegalovirus reactivation after allogeneic stem cell transplantation: comparison of an antigenemia assay and quantitative real-time polymerase chain reaction. Bone Marrow Transplantation, 29(7), 599-606.
Yakushiji K, et al. Monitoring of Cytomegalovirus Reactivation After Allogeneic Stem Cell Transplantation: Comparison of an Antigenemia Assay and Quantitative Real-time Polymerase Chain Reaction. Bone Marrow Transplant. 2002;29(7):599-606. PubMed PMID: 11979310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring of cytomegalovirus reactivation after allogeneic stem cell transplantation: comparison of an antigenemia assay and quantitative real-time polymerase chain reaction. AU - Yakushiji,K, AU - Gondo,H, AU - Kamezaki,K, AU - Shigematsu,K, AU - Hayashi,S, AU - Kuroiwa,M, AU - Taniguchi,S, AU - Ohno,Y, AU - Takase,K, AU - Numata,A, AU - Aoki,K, AU - Kato,K, AU - Nagafuji,K, AU - Shimoda,K, AU - Okamura,T, AU - Kinukawa,N, AU - Kasuga,N, AU - Sata,M, AU - Harada,M, PY - 2001/10/11/received PY - 2002/02/04/accepted PY - 2002/4/30/pubmed PY - 2002/9/26/medline PY - 2002/4/30/entrez SP - 599 EP - 606 JF - Bone marrow transplantation JO - Bone Marrow Transplant VL - 29 IS - 7 N2 - Cytomegalovirus (CMV) antigenemia and quantitative real-time polymerase chain reaction (PCR) were compared for monitoring of CMV reactivation after allogeneic stem cell transplantation. The number of CMV antigen-positive cells by the antigenemia assay and the level of CMV DNA by real-time PCR correlated well. The sensitivity and specificity of the antigenemia assay was 55.4% and 95.5%, respectively, using real-time PCR as the reference standard. The probability of positive antigenemia at day 100 was 76.5%, with a median of first detection at day 37 in 51 patients, compared with a positive PCR of 84.3% and day 33, respectively. When HLA-identical sibling donor transplant recipients and other donor transplant recipients were analyzed separately, there was no difference between the two tests. However, temporal patterns of first detection of CMV antigen-positive cells and CMV DNA differed between HLA-identical and alternative recipients; patients without CMV (29%) or with sporadic positive PCR results (14%) were more common in HLA-identical sibling transplants, whereas patients with simultaneous antigenemia and positive PCR occurred more in alternative transplants (48%). Two of 51 patients (4%) developed CMV colitis despite antigenemia-guided prophylaxis, but both were successfully treated with ganciclovir. Although PCR is more sensitive than antigenemia, both tests are useful in the early detection of CMV after allogeneic stem cell transplantation. SN - 0268-3369 UR - https://www.unboundmedicine.com/medline/citation/11979310/Monitoring_of_cytomegalovirus_reactivation_after_allogeneic_stem_cell_transplantation:_comparison_of_an_antigenemia_assay_and_quantitative_real_time_polymerase_chain_reaction_ L2 - https://doi.org/10.1038/sj.bmt.1703513 DB - PRIME DP - Unbound Medicine ER -