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Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture.
EMBO J. 2002 May 01; 21(9):2282-91.EJ

Abstract

Haploid Saccharomyces cells have the remarkable potential to change mating type as often as every generation, a process accomplished by an intrachromosomal gene conversion between an expressor locus MAT and one of two repositories of mating type information, HML or HMR. The particular locus selected as donor is dictated by the mating type of the cell, a bias that ensures productive mating type interconversion. Here we use green fluorescent protein tagging of the expressor and donor loci on chromosome III to show that this preference for donor locus does not result from a predetermined organization of chromosome III: HML and MAT as well as HMR and MAT remain separated in cells of both mating types. In fact, cells in which the inappropriate donor locus is artificially tethered to MAT still predominantly select the correct donor. We find, though, that initiation of switching leads to a rapid association of the correct donor locus with MAT. Thus, in mating type switching in Saccharomyces, donor preference is imposed at commitment to recombination rather than at physical contact of interacting DNA strands.

Authors+Show Affiliations

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11980725

Citation

Simon, Peter, et al. "Directional Bias During Mating Type Switching in Saccharomyces Is Independent of Chromosomal Architecture." The EMBO Journal, vol. 21, no. 9, 2002, pp. 2282-91.
Simon P, Houston P, Broach J. Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture. EMBO J. 2002;21(9):2282-91.
Simon, P., Houston, P., & Broach, J. (2002). Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture. The EMBO Journal, 21(9), 2282-91.
Simon P, Houston P, Broach J. Directional Bias During Mating Type Switching in Saccharomyces Is Independent of Chromosomal Architecture. EMBO J. 2002 May 1;21(9):2282-91. PubMed PMID: 11980725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture. AU - Simon,Peter, AU - Houston,Peter, AU - Broach,James, PY - 2002/5/1/pubmed PY - 2002/6/14/medline PY - 2002/5/1/entrez SP - 2282 EP - 91 JF - The EMBO journal JO - EMBO J VL - 21 IS - 9 N2 - Haploid Saccharomyces cells have the remarkable potential to change mating type as often as every generation, a process accomplished by an intrachromosomal gene conversion between an expressor locus MAT and one of two repositories of mating type information, HML or HMR. The particular locus selected as donor is dictated by the mating type of the cell, a bias that ensures productive mating type interconversion. Here we use green fluorescent protein tagging of the expressor and donor loci on chromosome III to show that this preference for donor locus does not result from a predetermined organization of chromosome III: HML and MAT as well as HMR and MAT remain separated in cells of both mating types. In fact, cells in which the inappropriate donor locus is artificially tethered to MAT still predominantly select the correct donor. We find, though, that initiation of switching leads to a rapid association of the correct donor locus with MAT. Thus, in mating type switching in Saccharomyces, donor preference is imposed at commitment to recombination rather than at physical contact of interacting DNA strands. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/11980725/Directional_bias_during_mating_type_switching_in_Saccharomyces_is_independent_of_chromosomal_architecture_ L2 - https://doi.org/10.1093/emboj/21.9.2282 DB - PRIME DP - Unbound Medicine ER -