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Interaction of exercise and diet on GLUT-4 protein and gene expression in Type I and Type II rat skeletal muscle.
Acta Physiol Scand. 2002 May; 175(1):37-44.AP

Abstract

We determined the interaction of exercise and diet on glucose transporter (GLUT-4) protein and mRNA expression in type I (soleus) and type II [extensor digitorum longus (EDL)] skeletal muscle. Forty-eight Sprague Dawley rats were randomly assigned to one of two dietary conditions: high-fat (FAT, n=24) or high-carbohydrate (CHO, n=24). Animals in each dietary condition were allocated to one of two groups: control (NT, n=8) or a group that performed 8 weeks of treadmill running (4 sessions week-1 of 1000 m @ 28 m min-1, RUN, n=16). Eight trained rats were killed after their final exercise bout for determination of GLUT-4 protein and mRNA expression: the remainder were killed 48 h after their last session for measurement of muscle glycogen and triacylglycerol concentration. GLUT-4 protein expression in NT rats was similar in both muscles after 8 weeks of either diet. However, there was a main effect of training such that GLUT-4 protein was increased in the soleus of rats fed with either diet (P < 0.05) and in the EDL in animals fed with CHO (P < 0.05). There was a significant diet-training interaction on GLUT-4 mRNA, such that expression was increased in both the soleus (100% upward arrowP < 0.05) and EDL (142% upward arrowP < 0.01) in CHO-fed animals. Trained rats fed with FAT decreased mRNA expression in the EDL (downward arrow 45%, P < 0.05) but not the soleus (downward arrow 14%, NS). We conclude that exercise training in CHO-fed rats increased both GLUT-4 protein and mRNA expression in type I and type II skeletal muscle. Despite lower GLUT-4 mRNA in muscles from fat-fed animals, exercise-induced increases in GLUT-4 protein were largely preserved, suggesting that control of GLUT-4 protein and gene expression are modified independently by exercise and diet.

Authors+Show Affiliations

Exercise Metabolism Group, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11982503

Citation

Lee, J S., et al. "Interaction of Exercise and Diet On GLUT-4 Protein and Gene Expression in Type I and Type II Rat Skeletal Muscle." Acta Physiologica Scandinavica, vol. 175, no. 1, 2002, pp. 37-44.
Lee JS, Bruce CR, Tunstall RJ, et al. Interaction of exercise and diet on GLUT-4 protein and gene expression in Type I and Type II rat skeletal muscle. Acta Physiol Scand. 2002;175(1):37-44.
Lee, J. S., Bruce, C. R., Tunstall, R. J., Cameron-Smith, D., Hugel, H., & Hawley, J. A. (2002). Interaction of exercise and diet on GLUT-4 protein and gene expression in Type I and Type II rat skeletal muscle. Acta Physiologica Scandinavica, 175(1), 37-44.
Lee JS, et al. Interaction of Exercise and Diet On GLUT-4 Protein and Gene Expression in Type I and Type II Rat Skeletal Muscle. Acta Physiol Scand. 2002;175(1):37-44. PubMed PMID: 11982503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction of exercise and diet on GLUT-4 protein and gene expression in Type I and Type II rat skeletal muscle. AU - Lee,J S, AU - Bruce,C R, AU - Tunstall,R J, AU - Cameron-Smith,D, AU - Hugel,H, AU - Hawley,J A, PY - 2002/5/2/pubmed PY - 2002/9/24/medline PY - 2002/5/2/entrez SP - 37 EP - 44 JF - Acta physiologica Scandinavica JO - Acta Physiol Scand VL - 175 IS - 1 N2 - We determined the interaction of exercise and diet on glucose transporter (GLUT-4) protein and mRNA expression in type I (soleus) and type II [extensor digitorum longus (EDL)] skeletal muscle. Forty-eight Sprague Dawley rats were randomly assigned to one of two dietary conditions: high-fat (FAT, n=24) or high-carbohydrate (CHO, n=24). Animals in each dietary condition were allocated to one of two groups: control (NT, n=8) or a group that performed 8 weeks of treadmill running (4 sessions week-1 of 1000 m @ 28 m min-1, RUN, n=16). Eight trained rats were killed after their final exercise bout for determination of GLUT-4 protein and mRNA expression: the remainder were killed 48 h after their last session for measurement of muscle glycogen and triacylglycerol concentration. GLUT-4 protein expression in NT rats was similar in both muscles after 8 weeks of either diet. However, there was a main effect of training such that GLUT-4 protein was increased in the soleus of rats fed with either diet (P < 0.05) and in the EDL in animals fed with CHO (P < 0.05). There was a significant diet-training interaction on GLUT-4 mRNA, such that expression was increased in both the soleus (100% upward arrowP < 0.05) and EDL (142% upward arrowP < 0.01) in CHO-fed animals. Trained rats fed with FAT decreased mRNA expression in the EDL (downward arrow 45%, P < 0.05) but not the soleus (downward arrow 14%, NS). We conclude that exercise training in CHO-fed rats increased both GLUT-4 protein and mRNA expression in type I and type II skeletal muscle. Despite lower GLUT-4 mRNA in muscles from fat-fed animals, exercise-induced increases in GLUT-4 protein were largely preserved, suggesting that control of GLUT-4 protein and gene expression are modified independently by exercise and diet. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/11982503/Interaction_of_exercise_and_diet_on_GLUT_4_protein_and_gene_expression_in_Type_I_and_Type_II_rat_skeletal_muscle_ L2 - https://doi.org/10.1046/j.1365-201X.2002.00963.x DB - PRIME DP - Unbound Medicine ER -