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Lactobacillic acid accumulation in the plasma membrane of Oenococcus oeni: a response to ethanol stress?
Microb Ecol 2002; 43(1):146-53ME

Abstract

It is known that ethanol strongly interferes with the development and activity of lactic acid bacteria in wine. In this work, it was observed that membrane composition was dependent of ethanol concentration and cell physiological state. The protein electrophoretic profile was modified in the membranes of Oenococcus oeni cultured in presence of 8 and 10% ethanol. Concerning the membrane lipid composition, it was observed that O. oeni maintained a high level of phospholipid biosynthesis via the relative increased biosynthesis of phosphoethanolamine and sphingomyelin in presence of ethanol. On the other hand, ethanol induced an increase in the membrane lactobacillic acid percentage at the expense of cis-vaccenic acid. This increased synthesis of lactobacillic acid appears as the more significant change induced by ethanol in O. oeni membrane. The increase of lactobacillic acid in the membrane of O. oeni clearly appears as a factor that provides protection against the toxic effect of ethanol, balancing the increase of membrane fluidity normally attributed to ethanol. The results presented in this paper constitute evidence that lactobacillic acid may have a part in the survival and or adaptive mechanisms developed by O. oeni under culture adverse conditions, allowing these bacteria to maintain their activity in the presence of ethanol, namely performing malolactic fermentation in wine.

Authors+Show Affiliations

Instituto de Biologia Experimental and Tecnologica, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apt 127, 2781-901 Oeiras, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11984636

Citation

Teixeira, H, et al. "Lactobacillic Acid Accumulation in the Plasma Membrane of Oenococcus Oeni: a Response to Ethanol Stress?" Microbial Ecology, vol. 43, no. 1, 2002, pp. 146-53.
Teixeira H, Gonçalves MG, Rozès N, et al. Lactobacillic acid accumulation in the plasma membrane of Oenococcus oeni: a response to ethanol stress? Microb Ecol. 2002;43(1):146-53.
Teixeira, H., Gonçalves, M. G., Rozès, N., Ramos, A., & San Romão, M. V. (2002). Lactobacillic acid accumulation in the plasma membrane of Oenococcus oeni: a response to ethanol stress? Microbial Ecology, 43(1), pp. 146-53.
Teixeira H, et al. Lactobacillic Acid Accumulation in the Plasma Membrane of Oenococcus Oeni: a Response to Ethanol Stress. Microb Ecol. 2002;43(1):146-53. PubMed PMID: 11984636.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lactobacillic acid accumulation in the plasma membrane of Oenococcus oeni: a response to ethanol stress? AU - Teixeira,H, AU - Gonçalves,M G, AU - Rozès,N, AU - Ramos,A, AU - San Romão,M V, Y1 - 2002/01/23/ PY - 2001/06/06/received PY - 2001/08/21/accepted PY - 2002/5/2/pubmed PY - 2002/7/31/medline PY - 2002/5/2/entrez SP - 146 EP - 53 JF - Microbial ecology JO - Microb. Ecol. VL - 43 IS - 1 N2 - It is known that ethanol strongly interferes with the development and activity of lactic acid bacteria in wine. In this work, it was observed that membrane composition was dependent of ethanol concentration and cell physiological state. The protein electrophoretic profile was modified in the membranes of Oenococcus oeni cultured in presence of 8 and 10% ethanol. Concerning the membrane lipid composition, it was observed that O. oeni maintained a high level of phospholipid biosynthesis via the relative increased biosynthesis of phosphoethanolamine and sphingomyelin in presence of ethanol. On the other hand, ethanol induced an increase in the membrane lactobacillic acid percentage at the expense of cis-vaccenic acid. This increased synthesis of lactobacillic acid appears as the more significant change induced by ethanol in O. oeni membrane. The increase of lactobacillic acid in the membrane of O. oeni clearly appears as a factor that provides protection against the toxic effect of ethanol, balancing the increase of membrane fluidity normally attributed to ethanol. The results presented in this paper constitute evidence that lactobacillic acid may have a part in the survival and or adaptive mechanisms developed by O. oeni under culture adverse conditions, allowing these bacteria to maintain their activity in the presence of ethanol, namely performing malolactic fermentation in wine. SN - 0095-3628 UR - https://www.unboundmedicine.com/medline/citation/11984636/Lactobacillic_acid_accumulation_in_the_plasma_membrane_of_Oenococcus_oeni:_a_response_to_ethanol_stress L2 - https://dx.doi.org/10.1007/s00248-001-0036-6 DB - PRIME DP - Unbound Medicine ER -