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Comparison between capillary electrophoresis and liquid chromatography for the determination of diclofenac sodium in a pharmaceutical tablet.
J AOAC Int. 2002 Mar-Apr; 85(2):333-40.JA

Abstract

Two novel analytical methodologies using capillary electrophoresis (CE) and liquid chromatography (LC) were developed and compared for the determination of diclofenac sodium in commercial and simulated tablet formulations. The CE analysis was performed in a bare fused-silica capillary with 75 microm id and total length of 50 cm (28 cm to the detector) with a buffer solution of 20 mM sodium tetraborate, pH 9.23. The applied voltage was 20 kV, and acetaminophen was used as the internal standard (IS). The LC analysis was performed with a LiChrospher 100 RP-18 (5 microm) column and a mobile phase of methanol-diluted glacial acetic acid (0.3 parts in 2500; 75 + 25) at a flow rate of 0.9 mL/min with propylparaben as the IS. In both analyses, detection was by ultraviolet absorption at 276 nm. Under optimized conditions, the CE migration times for the diclofenac sodium standard and acetaminophen (IS) were 2.07 and 1.59 min, respectively, and the LC retention times for the diclofenac sodium standard and propylparaben (IS) were 3.98 and 2.26 min, respectively. The resolution and efficiency for CE were 14.2 and 1.6 x 10(5) plates/m, respectively, and for LC, 5.0 and 8.6 x 10(3) plates/m, respectively. Calibration curves of peak area versus concentration gave correlation coefficients of 0.9992 for CE and 0.9994 for LC. The limits of detection and quantitation were 8.40 and 25.46 microg/mL, respectively, for CE and 4.60 and 13.93 microg/mL, respectively, for LC. Coefficients of variation were 1.68 and 0.37% for CE and LC, respectively. Average recoveries obtained with CE and LC were 103.12+/-0.90 and 99.59+/-0.21%, respectively. Although both methodologies were shown to be suitable for the determination of diclofenac sodium in tablets, performing in a similar manner with regard to several aspects (linearity, recovery, and specificity), CE provided faster analysis and better column efficiency, whereas LC provided superior repeatability and sensitivity.

Authors+Show Affiliations

Aurora-Prado MS
University of São Paulo, Faculty of Pharmaceutical Sciences, Department of Pharmacy, SP, Brazil.
Steppe M
No affiliation info available
Tavares MF
No affiliation info available
Kedor-Hackmann ER
No affiliation info available
Santoro MI
No affiliation info available

MeSH

Chromatography, LiquidDiclofenacElectrophoresis, CapillarySensitivity and SpecificityTablets

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11990016

Citation

Aurora-Prado, María S., et al. "Comparison Between Capillary Electrophoresis and Liquid Chromatography for the Determination of Diclofenac Sodium in a Pharmaceutical Tablet." Journal of AOAC International, vol. 85, no. 2, 2002, pp. 333-40.
Aurora-Prado MS, Steppe M, Tavares MF, et al. Comparison between capillary electrophoresis and liquid chromatography for the determination of diclofenac sodium in a pharmaceutical tablet. J AOAC Int. 2002;85(2):333-40.
Aurora-Prado, M. S., Steppe, M., Tavares, M. F., Kedor-Hackmann, E. R., & Santoro, M. I. (2002). Comparison between capillary electrophoresis and liquid chromatography for the determination of diclofenac sodium in a pharmaceutical tablet. Journal of AOAC International, 85(2), 333-40.
Aurora-Prado MS, et al. Comparison Between Capillary Electrophoresis and Liquid Chromatography for the Determination of Diclofenac Sodium in a Pharmaceutical Tablet. J AOAC Int. 2002 Mar-Apr;85(2):333-40. PubMed PMID: 11990016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison between capillary electrophoresis and liquid chromatography for the determination of diclofenac sodium in a pharmaceutical tablet. AU - Aurora-Prado,María S, AU - Steppe,Martin, AU - Tavares,Marina F M, AU - Kedor-Hackmann,Erika R M, AU - Santoro,Maria Inês R M, PY - 2002/5/7/pubmed PY - 2002/10/31/medline PY - 2002/5/7/entrez SP - 333 EP - 40 JF - Journal of AOAC International JO - J AOAC Int VL - 85 IS - 2 N2 - Two novel analytical methodologies using capillary electrophoresis (CE) and liquid chromatography (LC) were developed and compared for the determination of diclofenac sodium in commercial and simulated tablet formulations. The CE analysis was performed in a bare fused-silica capillary with 75 microm id and total length of 50 cm (28 cm to the detector) with a buffer solution of 20 mM sodium tetraborate, pH 9.23. The applied voltage was 20 kV, and acetaminophen was used as the internal standard (IS). The LC analysis was performed with a LiChrospher 100 RP-18 (5 microm) column and a mobile phase of methanol-diluted glacial acetic acid (0.3 parts in 2500; 75 + 25) at a flow rate of 0.9 mL/min with propylparaben as the IS. In both analyses, detection was by ultraviolet absorption at 276 nm. Under optimized conditions, the CE migration times for the diclofenac sodium standard and acetaminophen (IS) were 2.07 and 1.59 min, respectively, and the LC retention times for the diclofenac sodium standard and propylparaben (IS) were 3.98 and 2.26 min, respectively. The resolution and efficiency for CE were 14.2 and 1.6 x 10(5) plates/m, respectively, and for LC, 5.0 and 8.6 x 10(3) plates/m, respectively. Calibration curves of peak area versus concentration gave correlation coefficients of 0.9992 for CE and 0.9994 for LC. The limits of detection and quantitation were 8.40 and 25.46 microg/mL, respectively, for CE and 4.60 and 13.93 microg/mL, respectively, for LC. Coefficients of variation were 1.68 and 0.37% for CE and LC, respectively. Average recoveries obtained with CE and LC were 103.12+/-0.90 and 99.59+/-0.21%, respectively. Although both methodologies were shown to be suitable for the determination of diclofenac sodium in tablets, performing in a similar manner with regard to several aspects (linearity, recovery, and specificity), CE provided faster analysis and better column efficiency, whereas LC provided superior repeatability and sensitivity. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/11990016/Comparison_between_capillary_electrophoresis_and_liquid_chromatography_for_the_determination_of_diclofenac_sodium_in_a_pharmaceutical_tablet_ DB - PRIME DP - Unbound Medicine ER -