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Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction .
J Chromatogr A. 2002 Mar 15; 950(1-2):21-9.JC

Abstract

A method for separation of cytokinins from auxin and abscisic acid, which allows further separation of cytokinin ribotides from cytokinin bases, ribosides and glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationic cytokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluted by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (RIA) or HPLC-MS.

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Authors+Show Affiliations

Dobrev PI
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha.
Kamínek M
No affiliation info available

MeSH

Abscisic AcidChromatography, High Pressure LiquidCytokininsEnzyme-Linked Immunosorbent AssayIndoleacetic AcidsMass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11990994

Citation

Dobrev, Petre Ivanov, and Miroslav Kamínek. "Fast and Efficient Separation of Cytokinins From Auxin and Abscisic Acid and Their Purification Using Mixed-mode Solid-phase Extraction ." Journal of Chromatography. A, vol. 950, no. 1-2, 2002, pp. 21-9.
Dobrev PI, Kamínek M. Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction . J Chromatogr A. 2002;950(1-2):21-9.
Dobrev, P. I., & Kamínek, M. (2002). Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction . Journal of Chromatography. A, 950(1-2), 21-9.
Dobrev PI, Kamínek M. Fast and Efficient Separation of Cytokinins From Auxin and Abscisic Acid and Their Purification Using Mixed-mode Solid-phase Extraction. J Chromatogr A. 2002 Mar 15;950(1-2):21-9. PubMed PMID: 11990994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction . AU - Dobrev,Petre Ivanov, AU - Kamínek,Miroslav, PY - 2002/5/7/pubmed PY - 2002/11/26/medline PY - 2002/5/7/entrez SP - 21 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 950 IS - 1-2 N2 - A method for separation of cytokinins from auxin and abscisic acid, which allows further separation of cytokinin ribotides from cytokinin bases, ribosides and glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationic cytokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluted by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (RIA) or HPLC-MS. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/11990994/Fast_and_efficient_separation_of_cytokinins_from_auxin_and_abscisic_acid_and_their_purification_using_mixed_mode_solid_phase_extraction__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(02)00024-9 DB - PRIME DP - Unbound Medicine ER -