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Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure.
Nat Struct Biol. 2002 Jun; 9(6):431-5.NS

Abstract

U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.

Authors+Show Affiliations

Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11992125

Citation

Huppler, Anna, et al. "Metal Binding and Base Ionization in the U6 RNA Intramolecular Stem-loop Structure." Nature Structural Biology, vol. 9, no. 6, 2002, pp. 431-5.
Huppler A, Nikstad LJ, Allmann AM, et al. Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure. Nat Struct Biol. 2002;9(6):431-5.
Huppler, A., Nikstad, L. J., Allmann, A. M., Brow, D. A., & Butcher, S. E. (2002). Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure. Nature Structural Biology, 9(6), 431-5.
Huppler A, et al. Metal Binding and Base Ionization in the U6 RNA Intramolecular Stem-loop Structure. Nat Struct Biol. 2002;9(6):431-5. PubMed PMID: 11992125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure. AU - Huppler,Anna, AU - Nikstad,Laura J, AU - Allmann,Anne M, AU - Brow,David A, AU - Butcher,Samuel E, PY - 2002/5/7/pubmed PY - 2002/6/19/medline PY - 2002/5/7/entrez SP - 431 EP - 5 JF - Nature structural biology JO - Nat Struct Biol VL - 9 IS - 6 N2 - U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing. SN - 1072-8368 UR - https://www.unboundmedicine.com/medline/citation/11992125/Metal_binding_and_base_ionization_in_the_U6_RNA_intramolecular_stem_loop_structure_ L2 - https://facultyopinions.com/pubmed/11992125 DB - PRIME DP - Unbound Medicine ER -