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Delta(9)-tetrahydrocannabinol increases endogenous extracellular glutamate levels in primary cultures of rat cerebral cortex neurons: involvement of CB(1) receptors.

Abstract

The effects of the principal psychoactive component of marijuana, Delta(9)-tetrahydrocannabinol (Delta(9)-THC), on endogenous extracellular glutamate levels in primary cultures of rat cerebral cortex neurons were investigated. Locally applied Delta(9)-THC (0.03, 3, 300, and 1,000 nM) concentration-dependently increased basal extracellular glutamate levels (+18% +/- 11%, +54% +/- 10%, +90% +/- 14%, +149% +/- 33% vs. basal). The facilitatory effects of Delta(9)-THC (3 and 300 nM) on cortical glutamate were fully counteracted in the presence of the selective CB(1) receptor antagonist SR141716A (10 nM) and by replacement of the normal Krebs-Ringer bicarbonate buffer with a low-Ca(2+) (0.2 mM) medium. Delta(9)-THC application also induced an enhancement in K(+)-evoked glutamate levels. These findings suggest that an increase in cortical glutamatergic transmission mediated by local CB(1) receptor activation may underlie some of the psychoactive and behavioral effects of acute marijuana consumption.

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  • Authors+Show Affiliations

    ,

    Department of Clinical and Experimental Medicine, Pharmacology Section, University of Ferrara, Ferrara, Italy.

    , , , , ,

    Source

    Journal of neuroscience research 68:4 2002 May 15 pg 449-53

    MeSH

    Animals
    Cell Culture Techniques
    Cerebral Cortex
    Dronabinol
    Extracellular Space
    Glutamic Acid
    Piperidines
    Pyrazoles
    Rats
    Rats, Sprague-Dawley
    Receptors, Cannabinoid
    Receptors, Drug
    Rimonabant

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    11992471

    Citation

    Tomasini, Maria Cristina, et al. "Delta(9)-tetrahydrocannabinol Increases Endogenous Extracellular Glutamate Levels in Primary Cultures of Rat Cerebral Cortex Neurons: Involvement of CB(1) Receptors." Journal of Neuroscience Research, vol. 68, no. 4, 2002, pp. 449-53.
    Tomasini MC, Ferraro L, Bebe BW, et al. Delta(9)-tetrahydrocannabinol increases endogenous extracellular glutamate levels in primary cultures of rat cerebral cortex neurons: involvement of CB(1) receptors. J Neurosci Res. 2002;68(4):449-53.
    Tomasini, M. C., Ferraro, L., Bebe, B. W., Tanganelli, S., Cassano, T., Cuomo, V., & Antonelli, T. (2002). Delta(9)-tetrahydrocannabinol increases endogenous extracellular glutamate levels in primary cultures of rat cerebral cortex neurons: involvement of CB(1) receptors. Journal of Neuroscience Research, 68(4), pp. 449-53.
    Tomasini MC, et al. Delta(9)-tetrahydrocannabinol Increases Endogenous Extracellular Glutamate Levels in Primary Cultures of Rat Cerebral Cortex Neurons: Involvement of CB(1) Receptors. J Neurosci Res. 2002 May 15;68(4):449-53. PubMed PMID: 11992471.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Delta(9)-tetrahydrocannabinol increases endogenous extracellular glutamate levels in primary cultures of rat cerebral cortex neurons: involvement of CB(1) receptors. AU - Tomasini,Maria Cristina, AU - Ferraro,Luca, AU - Bebe,Berta Wonjie, AU - Tanganelli,Sergio, AU - Cassano,Tommaso, AU - Cuomo,Vincenzo, AU - Antonelli,Tiziana, PY - 2002/5/7/pubmed PY - 2002/7/19/medline PY - 2002/5/7/entrez SP - 449 EP - 53 JF - Journal of neuroscience research JO - J. Neurosci. Res. VL - 68 IS - 4 N2 - The effects of the principal psychoactive component of marijuana, Delta(9)-tetrahydrocannabinol (Delta(9)-THC), on endogenous extracellular glutamate levels in primary cultures of rat cerebral cortex neurons were investigated. Locally applied Delta(9)-THC (0.03, 3, 300, and 1,000 nM) concentration-dependently increased basal extracellular glutamate levels (+18% +/- 11%, +54% +/- 10%, +90% +/- 14%, +149% +/- 33% vs. basal). The facilitatory effects of Delta(9)-THC (3 and 300 nM) on cortical glutamate were fully counteracted in the presence of the selective CB(1) receptor antagonist SR141716A (10 nM) and by replacement of the normal Krebs-Ringer bicarbonate buffer with a low-Ca(2+) (0.2 mM) medium. Delta(9)-THC application also induced an enhancement in K(+)-evoked glutamate levels. These findings suggest that an increase in cortical glutamatergic transmission mediated by local CB(1) receptor activation may underlie some of the psychoactive and behavioral effects of acute marijuana consumption. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/11992471/Delta_9__tetrahydrocannabinol_increases_endogenous_extracellular_glutamate_levels_in_primary_cultures_of_rat_cerebral_cortex_neurons:_involvement_of_CB_1__receptors_ L2 - https://doi.org/10.1002/jnr.10242 DB - PRIME DP - Unbound Medicine ER -