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The effect of proteolytic enzymes on the vitamin B12-binding proteins of human gastric juice and saliva.
Scand J Gastroenterol. 1979; 14(7):833-8.SJ

Abstract

Pepsin had no effect on the vitamin B12 binder in human saliva (R-binder), while trypsin was found to reduce the apparent molecular weight of the R-binder and to release vitamin B12 from the R-B12complex of human saliva and human gastric juice (HGJ). Trypsin had no effect on the molecular weight and biological activity of intrinsic factor (IF) in HGJ, as demonstrated by gel filtration on Sephadex G-150 and the uptake of IF-B12 by guinea pig intestinal brush borders. An extract of purified guinea pig intestinal lysosomes was also without effect on the molecular weight and the biological activity of IF but was found to release vitamin B12 from the R-B12 complex. The results support the observation that the external pancreatic secretion corrects malabsorption of vitamin B12 by an effect on the non-IF protein in the intestinal juice. Moreover, the results indicate that lysosomal enzymes are not involved in the intestinal absorption of vitamin B12.

Authors

Andersen KJ
No affiliation info available
von der Lippe G
No affiliation info available

MeSH

AnimalsChromatography, GelGastric JuiceGuinea PigsHumansIleumIntestinal AbsorptionIntrinsic FactorLysosomesMolecular WeightPepsin AProtein BindingSalivary Proteins and PeptidesTrypsinVitamin B 12

Pub Type(s)

Journal Article

Language

eng

PubMed ID

120000

Citation

Andersen, K J., and G von der Lippe. "The Effect of Proteolytic Enzymes On the Vitamin B12-binding Proteins of Human Gastric Juice and Saliva." Scandinavian Journal of Gastroenterology, vol. 14, no. 7, 1979, pp. 833-8.
Andersen KJ, von der Lippe G. The effect of proteolytic enzymes on the vitamin B12-binding proteins of human gastric juice and saliva. Scand J Gastroenterol. 1979;14(7):833-8.
Andersen, K. J., & von der Lippe, G. (1979). The effect of proteolytic enzymes on the vitamin B12-binding proteins of human gastric juice and saliva. Scandinavian Journal of Gastroenterology, 14(7), 833-8.
Andersen KJ, von der Lippe G. The Effect of Proteolytic Enzymes On the Vitamin B12-binding Proteins of Human Gastric Juice and Saliva. Scand J Gastroenterol. 1979;14(7):833-8. PubMed PMID: 120000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of proteolytic enzymes on the vitamin B12-binding proteins of human gastric juice and saliva. AU - Andersen,K J, AU - von der Lippe,G, PY - 1979/1/1/pubmed PY - 1979/1/1/medline PY - 1979/1/1/entrez SP - 833 EP - 8 JF - Scandinavian journal of gastroenterology JO - Scand J Gastroenterol VL - 14 IS - 7 N2 - Pepsin had no effect on the vitamin B12 binder in human saliva (R-binder), while trypsin was found to reduce the apparent molecular weight of the R-binder and to release vitamin B12 from the R-B12complex of human saliva and human gastric juice (HGJ). Trypsin had no effect on the molecular weight and biological activity of intrinsic factor (IF) in HGJ, as demonstrated by gel filtration on Sephadex G-150 and the uptake of IF-B12 by guinea pig intestinal brush borders. An extract of purified guinea pig intestinal lysosomes was also without effect on the molecular weight and the biological activity of IF but was found to release vitamin B12 from the R-B12 complex. The results support the observation that the external pancreatic secretion corrects malabsorption of vitamin B12 by an effect on the non-IF protein in the intestinal juice. Moreover, the results indicate that lysosomal enzymes are not involved in the intestinal absorption of vitamin B12. SN - 0036-5521 UR - https://www.unboundmedicine.com/medline/citation/120000/The_effect_of_proteolytic_enzymes_on_the_vitamin_B12_binding_proteins_of_human_gastric_juice_and_saliva_ DB - PRIME DP - Unbound Medicine ER -