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Differential expression of dentin matrix protein 1, type I collagen and osteocalcin genes in rat developing mandibular bone.
Histochem J 2001 Sep-Oct; 33(9-10):545-52HJ

Abstract

The expression of dentin matrix protein 1 (Dmp1) mRNA has been compared with that of type I collagen and osteocalcin mRNAs during bone formation in the rat mandible, using in situ hybridization. At embryonic day 15 (E15), type I collagen and osteocalcin mRNAs were expressed by the majority of newly-differentiated osteoblasts attached to unmineralized bone matrices, whereas Dmp1 mRNA expression was confined to only a few osteoblasts. Expression of these genes increased as the number of osteoblasts increased in specimens from E16 to E18. At E20, expression of Dmp1, type I collagen and osteocalcin was also observed in osteocytes. Dmp1 expression continued in osteocytes as they matured up to the 90-day-old specimens, whereas type I collagen and osteocalcin expression in osteocytes almost disappeared at 30 days of postnatal life. In contrast, osteoblasts continued to express type I collagen and osteocalcin in 90-day-old rats, but transiently expressed Dmp1 mRNA, which was seen in the minority of osteoblasts at 14 days of postnatal life. These data show that the developmental expression patterns of Dmp1 in osteogenic differentiation differ from those of type I collagen and osteocalcin, and Dmp1 appears to be expressed by osteocytes throughout ossification in the skeleton. These observations indicate that Dmp1 may serve unique biological functions in osteocyte and bone metabolism.

Authors+Show Affiliations

Department of Anatomy, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12005026

Citation

Kamiya, N, and M Takagi. "Differential Expression of Dentin Matrix Protein 1, Type I Collagen and Osteocalcin Genes in Rat Developing Mandibular Bone." The Histochemical Journal, vol. 33, no. 9-10, 2001, pp. 545-52.
Kamiya N, Takagi M. Differential expression of dentin matrix protein 1, type I collagen and osteocalcin genes in rat developing mandibular bone. Histochem J. 2001;33(9-10):545-52.
Kamiya, N., & Takagi, M. (2001). Differential expression of dentin matrix protein 1, type I collagen and osteocalcin genes in rat developing mandibular bone. The Histochemical Journal, 33(9-10), pp. 545-52.
Kamiya N, Takagi M. Differential Expression of Dentin Matrix Protein 1, Type I Collagen and Osteocalcin Genes in Rat Developing Mandibular Bone. Histochem J. 2001;33(9-10):545-52. PubMed PMID: 12005026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of dentin matrix protein 1, type I collagen and osteocalcin genes in rat developing mandibular bone. AU - Kamiya,N, AU - Takagi,M, PY - 2002/5/15/pubmed PY - 2002/7/31/medline PY - 2002/5/15/entrez SP - 545 EP - 52 JF - The Histochemical journal JO - Histochem. J. VL - 33 IS - 9-10 N2 - The expression of dentin matrix protein 1 (Dmp1) mRNA has been compared with that of type I collagen and osteocalcin mRNAs during bone formation in the rat mandible, using in situ hybridization. At embryonic day 15 (E15), type I collagen and osteocalcin mRNAs were expressed by the majority of newly-differentiated osteoblasts attached to unmineralized bone matrices, whereas Dmp1 mRNA expression was confined to only a few osteoblasts. Expression of these genes increased as the number of osteoblasts increased in specimens from E16 to E18. At E20, expression of Dmp1, type I collagen and osteocalcin was also observed in osteocytes. Dmp1 expression continued in osteocytes as they matured up to the 90-day-old specimens, whereas type I collagen and osteocalcin expression in osteocytes almost disappeared at 30 days of postnatal life. In contrast, osteoblasts continued to express type I collagen and osteocalcin in 90-day-old rats, but transiently expressed Dmp1 mRNA, which was seen in the minority of osteoblasts at 14 days of postnatal life. These data show that the developmental expression patterns of Dmp1 in osteogenic differentiation differ from those of type I collagen and osteocalcin, and Dmp1 appears to be expressed by osteocytes throughout ossification in the skeleton. These observations indicate that Dmp1 may serve unique biological functions in osteocyte and bone metabolism. SN - 0018-2214 UR - https://www.unboundmedicine.com/medline/citation/12005026/Differential_expression_of_dentin_matrix_protein_1_type_I_collagen_and_osteocalcin_genes_in_rat_developing_mandibular_bone_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=12005026.ui DB - PRIME DP - Unbound Medicine ER -