Tags

Type your tag names separated by a space and hit enter

Endothelin-1 in citric acid aerosol inhalation-induced airway constriction of guinea pigs.
Chin J Physiol. 2002 Mar 31; 45(1):25-31.CJ

Abstract

Endotheline-1 (ET-1) has been shown to enhance tachykinin-induced airway constriction. This study was designed to test whether ET-1 is involved in citric acid-induced bronchoconstriction. Forty-eight anesthetized-paralyzed guinea pigs were divided into six groups of 8 animals each: saline control; citric acid; ET-1; ET-1 + citric acid; BQ123 + ET-1 + citric acid; and BQ788 + ET-1 + citric acid. BQ123 and BQ788 are specific ETA and ETB receptor antagonists, respectively. Each animal in the saline control group received 50 breaths of 4 ml saline aerosol and in all citric acid-treated groups was given 50 breaths of 4 ml aerosol generated from 0.6 M citric acid. In all ET-1-treated groups, each animal was exposed to aerosol generated from 10(-8) M ET-1. The animal in the ET-1 + citric acid group was exposed to ET-1 5 min prior to the citric acid. For the last two groups, each animal was first exposed to aerosol generated from either 10(-5) M BQ123 or 10(-5) M BQ788. Five min later, the animal was exposed to ET-1; and then 5 min later was followed by citric acid. Dynamic respiratory compliance (Crs), forced expiratory volume in 0.1 sec (FEV(0.1)), and maximal expiratory flow at 30% total lung capacity (Vmax 30) were obtained before and 3-15 min after citric acid. Either citric acid or ET-1 inhalation caused significant decreases in Crs, FEV(0.1), and Vmax 30, indicating airway constriction. Citric acid-induced airway constriction, for most cases, was not significantly augmented by ET-1. However, either BQ123 or BQ 788 significantly attenuated the airway constriction induced by the combination of ET-1 and citric acid. Also, in an additional study, either BQ123 or BQ788 significantly attenuated citric acid-induced airway constriction. These data suggest that endogenous ET-1 plays an important role in citric acid aerosol-induced airway constriction in guinea pigs.

Authors+Show Affiliations

Department of Physiology, College of Medicine, National Taiwan University, Taipei, ROC.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12005349

Citation

Lee, S C., and Y L. Lai. "Endothelin-1 in Citric Acid Aerosol Inhalation-induced Airway Constriction of Guinea Pigs." The Chinese Journal of Physiology, vol. 45, no. 1, 2002, pp. 25-31.
Lee SC, Lai YL. Endothelin-1 in citric acid aerosol inhalation-induced airway constriction of guinea pigs. Chin J Physiol. 2002;45(1):25-31.
Lee, S. C., & Lai, Y. L. (2002). Endothelin-1 in citric acid aerosol inhalation-induced airway constriction of guinea pigs. The Chinese Journal of Physiology, 45(1), 25-31.
Lee SC, Lai YL. Endothelin-1 in Citric Acid Aerosol Inhalation-induced Airway Constriction of Guinea Pigs. Chin J Physiol. 2002 Mar 31;45(1):25-31. PubMed PMID: 12005349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelin-1 in citric acid aerosol inhalation-induced airway constriction of guinea pigs. AU - Lee,S C, AU - Lai,Y L, PY - 2002/5/15/pubmed PY - 2002/10/31/medline PY - 2002/5/15/entrez SP - 25 EP - 31 JF - The Chinese journal of physiology JO - Chin J Physiol VL - 45 IS - 1 N2 - Endotheline-1 (ET-1) has been shown to enhance tachykinin-induced airway constriction. This study was designed to test whether ET-1 is involved in citric acid-induced bronchoconstriction. Forty-eight anesthetized-paralyzed guinea pigs were divided into six groups of 8 animals each: saline control; citric acid; ET-1; ET-1 + citric acid; BQ123 + ET-1 + citric acid; and BQ788 + ET-1 + citric acid. BQ123 and BQ788 are specific ETA and ETB receptor antagonists, respectively. Each animal in the saline control group received 50 breaths of 4 ml saline aerosol and in all citric acid-treated groups was given 50 breaths of 4 ml aerosol generated from 0.6 M citric acid. In all ET-1-treated groups, each animal was exposed to aerosol generated from 10(-8) M ET-1. The animal in the ET-1 + citric acid group was exposed to ET-1 5 min prior to the citric acid. For the last two groups, each animal was first exposed to aerosol generated from either 10(-5) M BQ123 or 10(-5) M BQ788. Five min later, the animal was exposed to ET-1; and then 5 min later was followed by citric acid. Dynamic respiratory compliance (Crs), forced expiratory volume in 0.1 sec (FEV(0.1)), and maximal expiratory flow at 30% total lung capacity (Vmax 30) were obtained before and 3-15 min after citric acid. Either citric acid or ET-1 inhalation caused significant decreases in Crs, FEV(0.1), and Vmax 30, indicating airway constriction. Citric acid-induced airway constriction, for most cases, was not significantly augmented by ET-1. However, either BQ123 or BQ 788 significantly attenuated the airway constriction induced by the combination of ET-1 and citric acid. Also, in an additional study, either BQ123 or BQ788 significantly attenuated citric acid-induced airway constriction. These data suggest that endogenous ET-1 plays an important role in citric acid aerosol-induced airway constriction in guinea pigs. SN - 0304-4920 UR - https://www.unboundmedicine.com/medline/citation/12005349/Endothelin_1_in_citric_acid_aerosol_inhalation_induced_airway_constriction_of_guinea_pigs_ DB - PRIME DP - Unbound Medicine ER -