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Isoprenoid biosynthesis in higher plants and in Escherichia coli: on the branching in the methylerythritol phosphate pathway and the independent biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate.
Biochem J. 2002 Sep 01; 366(Pt 2):573-83.BJ

Abstract

In the bacterium Escherichia coli, the mevalonic-acid (MVA)-independent 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is characterized by two branches leading separately to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The signature of this branching is the retention of deuterium in DMAPP and the deuterium loss in IPP after incorporation of 1-[4-(2)H]deoxy-d-xylulose ([4-(2)H]DX). Feeding tobacco BY-2 cell-suspension cultures with [4-(2)H]DX resulted in deuterium retention in the isoprene units derived from DMAPP, as well as from IPP in the plastidial isoprenoids, phytoene and plastoquinone, synthesized via the MEP pathway. This labelling pattern represents direct evidence for the presence of the DMAPP branch of the MEP pathway in a higher plant, and shows that IPP can be synthesized from DMAPP in plant plastids, most probably via a plastidial IPP isomerase.

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Authors+Show Affiliations

Hoeffler JF
Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg cedex, France.
Hemmerlin A
No affiliation info available
Grosdemange-Billiard C
No affiliation info available
Bach TJ
No affiliation info available
Rohmer M
No affiliation info available

MeSH

Cell LineEscherichia coliHemiterpenesIndicators and ReagentsOrganophosphorus CompoundsPlantsPolyisoprenyl PhosphatesTobaccoTritium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12010124

Citation

Hoeffler, Jean-François, et al. "Isoprenoid Biosynthesis in Higher Plants and in Escherichia Coli: On the Branching in the Methylerythritol Phosphate Pathway and the Independent Biosynthesis of Isopentenyl Diphosphate and Dimethylallyl Diphosphate." The Biochemical Journal, vol. 366, no. Pt 2, 2002, pp. 573-83.
Hoeffler JF, Hemmerlin A, Grosdemange-Billiard C, et al. Isoprenoid biosynthesis in higher plants and in Escherichia coli: on the branching in the methylerythritol phosphate pathway and the independent biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate. Biochem J. 2002;366(Pt 2):573-83.
Hoeffler, J. F., Hemmerlin, A., Grosdemange-Billiard, C., Bach, T. J., & Rohmer, M. (2002). Isoprenoid biosynthesis in higher plants and in Escherichia coli: on the branching in the methylerythritol phosphate pathway and the independent biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate. The Biochemical Journal, 366(Pt 2), 573-83.
Hoeffler JF, et al. Isoprenoid Biosynthesis in Higher Plants and in Escherichia Coli: On the Branching in the Methylerythritol Phosphate Pathway and the Independent Biosynthesis of Isopentenyl Diphosphate and Dimethylallyl Diphosphate. Biochem J. 2002 Sep 1;366(Pt 2):573-83. PubMed PMID: 12010124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoprenoid biosynthesis in higher plants and in Escherichia coli: on the branching in the methylerythritol phosphate pathway and the independent biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate. AU - Hoeffler,Jean-François, AU - Hemmerlin,Andréa, AU - Grosdemange-Billiard,Catherine, AU - Bach,Thomas J, AU - Rohmer,Michel, PY - 2002/05/15/accepted PY - 2002/04/26/revised PY - 2002/02/27/received PY - 2002/5/16/pubmed PY - 2002/10/18/medline PY - 2002/5/16/entrez SP - 573 EP - 83 JF - The Biochemical journal JO - Biochem J VL - 366 IS - Pt 2 N2 - In the bacterium Escherichia coli, the mevalonic-acid (MVA)-independent 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is characterized by two branches leading separately to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The signature of this branching is the retention of deuterium in DMAPP and the deuterium loss in IPP after incorporation of 1-[4-(2)H]deoxy-d-xylulose ([4-(2)H]DX). Feeding tobacco BY-2 cell-suspension cultures with [4-(2)H]DX resulted in deuterium retention in the isoprene units derived from DMAPP, as well as from IPP in the plastidial isoprenoids, phytoene and plastoquinone, synthesized via the MEP pathway. This labelling pattern represents direct evidence for the presence of the DMAPP branch of the MEP pathway in a higher plant, and shows that IPP can be synthesized from DMAPP in plant plastids, most probably via a plastidial IPP isomerase. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/12010124/Isoprenoid_biosynthesis_in_higher_plants_and_in_Escherichia_coli:_on_the_branching_in_the_methylerythritol_phosphate_pathway_and_the_independent_biosynthesis_of_isopentenyl_diphosphate_and_dimethylallyl_diphosphate_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BJ20020337 DB - PRIME DP - Unbound Medicine ER -